Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study
Cockle shells of the Anadara granosa species consist of 95-99% aragonite form of calcium carbonate (CaCO3) crystals. Aragonites are known to be denser which allows it to be incorporated, resolved and replaced by bone tissues over time when used in bone tissue grafting. An injectable biomaterial-base...
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| Format: | Article |
| Language: | English |
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Biome Scientia
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119351/ http://psasir.upm.edu.my/id/eprint/119351/1/119351.pdf |
| _version_ | 1848867941602295808 |
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| author | George, Penny Abdul Hamid, Zariyantey Abu Bakar, Md Zuki Bharatham, B. Hemabarathy |
| author_facet | George, Penny Abdul Hamid, Zariyantey Abu Bakar, Md Zuki Bharatham, B. Hemabarathy |
| author_sort | George, Penny |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cockle shells of the Anadara granosa species consist of 95-99% aragonite form of calcium carbonate (CaCO3) crystals. Aragonites are known to be denser which allows it to be incorporated, resolved and replaced by bone tissues over time when used in bone tissue grafting. An injectable biomaterial-based bone filler with appropriate injectability and biological properties was formulated using powdered cockle shells in nanoscale (nCSP) and sodium alginate (Alg). Initial attempts in formulating the bone filler using alginate and citric acid as a setting agent in the composition of nCSP: Alg of 60: 40, 70: 30 and 80: 20 wt.% produced variable results in the bone filler characterization. Characterization study of nCSP-Alg bone filler with 70:30 wt.% compositions showed excellent injectability (p<0.05), viscosity (p<0.05) and anti-washout ability that was further evaluated through physicochemical analysis, morphology and biocompatibility studies. Scanning electron micrographs revealed plate-like nanocrystal deposits with micropores ranging between 1.5 – 7.4 μm. XRD and FTIR evaluation indicated the presences of peaks associated with aragonite form of CaCO3. Biocompatibility studies with MG63 osteoblast showed osteoconductivity of the bone fillers with excellent cell adherence, growth and subsequent mineralization of the matrices. In conclusion, nCSP-Alg biomaterial-based bone filler with 70:30 wt.% compositions shows promising use as a cost effective bone grafting material for clinical application in the field of bone tissue engineering. |
| first_indexed | 2025-11-15T14:44:30Z |
| format | Article |
| id | upm-119351 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:44:30Z |
| publishDate | 2024 |
| publisher | Biome Scientia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1193512025-08-18T00:16:42Z http://psasir.upm.edu.my/id/eprint/119351/ Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study George, Penny Abdul Hamid, Zariyantey Abu Bakar, Md Zuki Bharatham, B. Hemabarathy Cockle shells of the Anadara granosa species consist of 95-99% aragonite form of calcium carbonate (CaCO3) crystals. Aragonites are known to be denser which allows it to be incorporated, resolved and replaced by bone tissues over time when used in bone tissue grafting. An injectable biomaterial-based bone filler with appropriate injectability and biological properties was formulated using powdered cockle shells in nanoscale (nCSP) and sodium alginate (Alg). Initial attempts in formulating the bone filler using alginate and citric acid as a setting agent in the composition of nCSP: Alg of 60: 40, 70: 30 and 80: 20 wt.% produced variable results in the bone filler characterization. Characterization study of nCSP-Alg bone filler with 70:30 wt.% compositions showed excellent injectability (p<0.05), viscosity (p<0.05) and anti-washout ability that was further evaluated through physicochemical analysis, morphology and biocompatibility studies. Scanning electron micrographs revealed plate-like nanocrystal deposits with micropores ranging between 1.5 – 7.4 μm. XRD and FTIR evaluation indicated the presences of peaks associated with aragonite form of CaCO3. Biocompatibility studies with MG63 osteoblast showed osteoconductivity of the bone fillers with excellent cell adherence, growth and subsequent mineralization of the matrices. In conclusion, nCSP-Alg biomaterial-based bone filler with 70:30 wt.% compositions shows promising use as a cost effective bone grafting material for clinical application in the field of bone tissue engineering. Biome Scientia 2024-05-31 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/119351/1/119351.pdf George, Penny and Abdul Hamid, Zariyantey and Abu Bakar, Md Zuki and Bharatham, B. Hemabarathy (2024) Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study. Life Sciences, Medicine and Biomedicine, 8 (1). art. no. 138. ISSN 2600-7207 https://biomescientia.com/index.php/lsmb/article/view/138 10.28916/lsmb.8.1.2024.138 |
| spellingShingle | George, Penny Abdul Hamid, Zariyantey Abu Bakar, Md Zuki Bharatham, B. Hemabarathy Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title | Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title_full | Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title_fullStr | Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title_full_unstemmed | Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title_short | Nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| title_sort | nano-cockle shell powder and alginate as novel injectable bone filler: a preliminary formulation and characterization study |
| url | http://psasir.upm.edu.my/id/eprint/119351/ http://psasir.upm.edu.my/id/eprint/119351/ http://psasir.upm.edu.my/id/eprint/119351/ http://psasir.upm.edu.my/id/eprint/119351/1/119351.pdf |