Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia
In late 2017, Malaysia reported repeated outbreaks of low-pathogenic avian influenza virus (LPAI) H9N2 infections in commercial poultry flocks. Two H9N2 viruses, A/chicken/Malaysia/Negeri Sembilan/UPM994/2018 and A/chicken/Malaysia/Johore/UPM2033/2019, which were isolated from breeder and layer floc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119348/ http://psasir.upm.edu.my/id/eprint/119348/1/119348.pdf |
| _version_ | 1848867940769726464 |
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| author | Gunasekara, Erandi Hair-Bejo, M. Aini, I. Omar, A. R. |
| author_facet | Gunasekara, Erandi Hair-Bejo, M. Aini, I. Omar, A. R. |
| author_sort | Gunasekara, Erandi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In late 2017, Malaysia reported repeated outbreaks of low-pathogenic avian influenza virus (LPAI) H9N2 infections in commercial poultry flocks. Two H9N2 viruses, A/chicken/Malaysia/Negeri Sembilan/UPM994/2018 and A/chicken/Malaysia/Johore/UPM2033/2019, which were isolated from breeder and layer flocks in Peninsular Malaysia, were characterised in this study. Phylogenetic analysis revealed that both viruses were multiple-genotype reassortant strains with genes originating from Y280-like (HA gene), F/98-like (NS, NP and PA), G1-like (M and PB2), and Korean-like (PB1) lineages, indicating that they belong to a novel genotype that is divergent from the G57 lineage of Chinese origin. Both isolates were predicted to have a dibasic cleavage site (333-PSRSSRGLF-341) in the HA gene cleavage locations. Thus, the novel Malaysian H9N2 strain is a Y280-like virus resembling H9N2 isolates from Indonesia, Taiwan, Japan, and Cambodia. This virus is of the G57 lineage but has a novel genotype of the PB1 gene originating from a Korean-lineage H9N2 virus, which has not been detected before in the region. |
| first_indexed | 2025-11-15T14:44:29Z |
| format | Article |
| id | upm-119348 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:44:29Z |
| publishDate | 2024 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1193482025-08-15T03:48:31Z http://psasir.upm.edu.my/id/eprint/119348/ Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia Gunasekara, Erandi Hair-Bejo, M. Aini, I. Omar, A. R. In late 2017, Malaysia reported repeated outbreaks of low-pathogenic avian influenza virus (LPAI) H9N2 infections in commercial poultry flocks. Two H9N2 viruses, A/chicken/Malaysia/Negeri Sembilan/UPM994/2018 and A/chicken/Malaysia/Johore/UPM2033/2019, which were isolated from breeder and layer flocks in Peninsular Malaysia, were characterised in this study. Phylogenetic analysis revealed that both viruses were multiple-genotype reassortant strains with genes originating from Y280-like (HA gene), F/98-like (NS, NP and PA), G1-like (M and PB2), and Korean-like (PB1) lineages, indicating that they belong to a novel genotype that is divergent from the G57 lineage of Chinese origin. Both isolates were predicted to have a dibasic cleavage site (333-PSRSSRGLF-341) in the HA gene cleavage locations. Thus, the novel Malaysian H9N2 strain is a Y280-like virus resembling H9N2 isolates from Indonesia, Taiwan, Japan, and Cambodia. This virus is of the G57 lineage but has a novel genotype of the PB1 gene originating from a Korean-lineage H9N2 virus, which has not been detected before in the region. Springer 2024-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/119348/1/119348.pdf Gunasekara, Erandi and Hair-Bejo, M. and Aini, I. and Omar, A. R. (2024) Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia. Archives of Virology, 170. art. no. 3. pp. 1-6. ISSN 0304-8608; eISSN: 1432-8798 https://link.springer.com/article/10.1007/s00705-024-06159-4?error=cookies_not_supported&code=d5e833cc-95af-49d3-b12e-9ecaf548da44 10.1007/s00705-024-06159-4 |
| spellingShingle | Gunasekara, Erandi Hair-Bejo, M. Aini, I. Omar, A. R. Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title | Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title_full | Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title_fullStr | Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title_full_unstemmed | Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title_short | Molecular characterisation of novel reassortants of the G57 genotype of low-pathogenic avian influenza H9N2 virus isolated from poultry farms in Malaysia |
| title_sort | molecular characterisation of novel reassortants of the g57 genotype of low-pathogenic avian influenza h9n2 virus isolated from poultry farms in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/119348/ http://psasir.upm.edu.my/id/eprint/119348/ http://psasir.upm.edu.my/id/eprint/119348/ http://psasir.upm.edu.my/id/eprint/119348/1/119348.pdf |