Duplex detection of avian and chicken using common primer-PCR in processed meat products
Meat fraudulent practice has been proven to damage the quality of the food and consumer’s health. Being widely available at lower cost, chicken meat has been frequently used as meat substitute in meat-based food products. As a solution, a duplex polymerase chain reaction (PCR) assay employing a comm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA Pahang, Malaysia
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119231/ http://psasir.upm.edu.my/id/eprint/119231/1/119231.pdf |
| _version_ | 1848867910396674048 |
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| author | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mohd Desa, Mohd Nasir Hanapi, Ummi Kalthum |
| author_facet | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mohd Desa, Mohd Nasir Hanapi, Ummi Kalthum |
| author_sort | Mohamad, Nurhidayatul Asma |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Meat fraudulent practice has been proven to damage the quality of the food and consumer’s health. Being widely available at lower cost, chicken meat has been frequently used as meat substitute in meat-based food products. As a solution, a duplex polymerase chain reaction (PCR) assay employing a common primer coupled with a unique adapter sequence was developed for simultaneous chicken-specific and avian universal detection. The assay targeting 322 and 219 bp of melanocortin 1 receptor (MC1R) gene sequence respectively was optimized with high specificity against a range of avian and meat species, and high LOD of 0.01 ng. Across forty-two commercial meat products tested, chicken meat was detected in seven non-avian samples where only two of them declared the presence of chicken meat in the label. The practical duplex PCR assay can be potentially applied for routine practice for qualitative investigation of contamination or adulteration in processed meat products. |
| first_indexed | 2025-11-15T14:44:00Z |
| format | Article |
| id | upm-119231 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:44:00Z |
| publishDate | 2024 |
| publisher | Universiti Teknologi MARA Pahang, Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1192312025-08-11T05:04:14Z http://psasir.upm.edu.my/id/eprint/119231/ Duplex detection of avian and chicken using common primer-PCR in processed meat products Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mohd Desa, Mohd Nasir Hanapi, Ummi Kalthum Meat fraudulent practice has been proven to damage the quality of the food and consumer’s health. Being widely available at lower cost, chicken meat has been frequently used as meat substitute in meat-based food products. As a solution, a duplex polymerase chain reaction (PCR) assay employing a common primer coupled with a unique adapter sequence was developed for simultaneous chicken-specific and avian universal detection. The assay targeting 322 and 219 bp of melanocortin 1 receptor (MC1R) gene sequence respectively was optimized with high specificity against a range of avian and meat species, and high LOD of 0.01 ng. Across forty-two commercial meat products tested, chicken meat was detected in seven non-avian samples where only two of them declared the presence of chicken meat in the label. The practical duplex PCR assay can be potentially applied for routine practice for qualitative investigation of contamination or adulteration in processed meat products. Universiti Teknologi MARA Pahang, Malaysia 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/119231/1/119231.pdf Mohamad, Nurhidayatul Asma and Khairil Mokhtar, Nur Fadhilah and Mohd Desa, Mohd Nasir and Hanapi, Ummi Kalthum (2024) Duplex detection of avian and chicken using common primer-PCR in processed meat products. Gading Journal of Science and Technology, 7 (1). pp. 29-43. ISSN 2637-0018 https://myjms.mohe.gov.my/index.php/gjst/article/view/24394 |
| spellingShingle | Mohamad, Nurhidayatul Asma Khairil Mokhtar, Nur Fadhilah Mohd Desa, Mohd Nasir Hanapi, Ummi Kalthum Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title | Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title_full | Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title_fullStr | Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title_full_unstemmed | Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title_short | Duplex detection of avian and chicken using common primer-PCR in processed meat products |
| title_sort | duplex detection of avian and chicken using common primer-pcr in processed meat products |
| url | http://psasir.upm.edu.my/id/eprint/119231/ http://psasir.upm.edu.my/id/eprint/119231/ http://psasir.upm.edu.my/id/eprint/119231/1/119231.pdf |