Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control
Aims: The present work aimed to distinguish the indigenous Aspergillus flavus isolates obtained from the first (pioneer) grain corn farms in Terengganu, Malaysia, into aflatoxigenic and non-aflatoxigenic by molecular and aflatoxigenicity analyses, and determine the antagonistic capability of the non...
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| Format: | Article |
| Language: | English |
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Oxford University Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119194/ http://psasir.upm.edu.my/id/eprint/119194/1/119194.pdf |
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| author | Yazid, Siti Nur Ezzati Selamat, Jinap Ismail, Siti Izera Sanny, Maimunah Samsudin, Nik Iskandar Putra |
| author_facet | Yazid, Siti Nur Ezzati Selamat, Jinap Ismail, Siti Izera Sanny, Maimunah Samsudin, Nik Iskandar Putra |
| author_sort | Yazid, Siti Nur Ezzati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aims: The present work aimed to distinguish the indigenous Aspergillus flavus isolates obtained from the first (pioneer) grain corn farms in Terengganu, Malaysia, into aflatoxigenic and non-aflatoxigenic by molecular and aflatoxigenicity analyses, and determine the antagonistic capability of the non-aflatoxigenic isolates against aflatoxigenic counterparts and their aflatoxin production in vitro. Methods and results: Seven A. flavus isolates previously obtained from the farms were characterized molecularly and chemically. All isolates were examined for the presence of seven aflatoxin biosynthesis genes, and their aflatoxigenicity was confirmed using high performance liquid chromatography with fluorescence detector. Phylogenetic relationships of all isolates were tested using ITS and β-tubulin genes. Of the seven isolates, two were non-aflatoxigenic, while the remaining were aflatoxigenic based on the presence of all aflatoxin biosynthesis genes tested and the productions of aflatoxins B1 and B2. All isolates were also confirmed as A. flavus following phylogenetic analysis. The indigenous non-aflatoxigenic isolates were further examined for their antagonistic potential against aflatoxigenic isolates on 3% grain corn agar. Both nonaflatoxigenic isolates significantly reduced AFB1 production of the aflatoxigenic isolates. Conclusion: The indigenous non-aflatoxigenic A. flavus strains identified in the present work were effective in controlling the aflatoxin production by the aflatoxigenic A. flavus isolates in vitro and can be utilized for in situ testing. |
| first_indexed | 2025-11-15T14:43:50Z |
| format | Article |
| id | upm-119194 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:43:50Z |
| publishDate | 2024 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1191942025-08-07T08:28:10Z http://psasir.upm.edu.my/id/eprint/119194/ Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control Yazid, Siti Nur Ezzati Selamat, Jinap Ismail, Siti Izera Sanny, Maimunah Samsudin, Nik Iskandar Putra Aims: The present work aimed to distinguish the indigenous Aspergillus flavus isolates obtained from the first (pioneer) grain corn farms in Terengganu, Malaysia, into aflatoxigenic and non-aflatoxigenic by molecular and aflatoxigenicity analyses, and determine the antagonistic capability of the non-aflatoxigenic isolates against aflatoxigenic counterparts and their aflatoxin production in vitro. Methods and results: Seven A. flavus isolates previously obtained from the farms were characterized molecularly and chemically. All isolates were examined for the presence of seven aflatoxin biosynthesis genes, and their aflatoxigenicity was confirmed using high performance liquid chromatography with fluorescence detector. Phylogenetic relationships of all isolates were tested using ITS and β-tubulin genes. Of the seven isolates, two were non-aflatoxigenic, while the remaining were aflatoxigenic based on the presence of all aflatoxin biosynthesis genes tested and the productions of aflatoxins B1 and B2. All isolates were also confirmed as A. flavus following phylogenetic analysis. The indigenous non-aflatoxigenic isolates were further examined for their antagonistic potential against aflatoxigenic isolates on 3% grain corn agar. Both nonaflatoxigenic isolates significantly reduced AFB1 production of the aflatoxigenic isolates. Conclusion: The indigenous non-aflatoxigenic A. flavus strains identified in the present work were effective in controlling the aflatoxin production by the aflatoxigenic A. flavus isolates in vitro and can be utilized for in situ testing. Oxford University Press 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/119194/1/119194.pdf Yazid, Siti Nur Ezzati and Selamat, Jinap and Ismail, Siti Izera and Sanny, Maimunah and Samsudin, Nik Iskandar Putra (2024) Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control. Journal of Applied Microbiology, 135 (6). art. no. lxae145. pp. 1-11. ISSN 1364-5072; eISSN: 1365-2672 https://academic.oup.com/jambio/article/doi/10.1093/jambio/lxae145/7693740 10.1093/jambio/lxae145 |
| spellingShingle | Yazid, Siti Nur Ezzati Selamat, Jinap Ismail, Siti Izera Sanny, Maimunah Samsudin, Nik Iskandar Putra Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title | Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title_full | Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title_fullStr | Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title_full_unstemmed | Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title_short | Molecular and aflatoxigenicity analyses of Aspergillus flavus isolates indigenous to grain corn in Malaysia; potentials for biological control |
| title_sort | molecular and aflatoxigenicity analyses of aspergillus flavus isolates indigenous to grain corn in malaysia; potentials for biological control |
| url | http://psasir.upm.edu.my/id/eprint/119194/ http://psasir.upm.edu.my/id/eprint/119194/ http://psasir.upm.edu.my/id/eprint/119194/ http://psasir.upm.edu.my/id/eprint/119194/1/119194.pdf |