The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study
The present study examined the vascular effects of peppermint or mint (Mentha longifolia L.) using an abdominal aortic rings model. Concentration-response curves for mint oil were generated after precontracting isolated mouse aorta with phenylephrine. The effect of different receptor antagonists and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Taylor and Francis
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/119137/ |
| _version_ | 1848867884067979264 |
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| author | Alkharfy, Khalid M. Ahmad, Ajaz Almuaijel, Saleh Hashim, Abdullah Raish, Mohammad Jan, Basit L. Rehman, Najeeb Ur Anwar, Farooq Rehman, Md Tabish Alajmi, Mohamad F. |
| author_facet | Alkharfy, Khalid M. Ahmad, Ajaz Almuaijel, Saleh Hashim, Abdullah Raish, Mohammad Jan, Basit L. Rehman, Najeeb Ur Anwar, Farooq Rehman, Md Tabish Alajmi, Mohamad F. |
| author_sort | Alkharfy, Khalid M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study examined the vascular effects of peppermint or mint (Mentha longifolia L.) using an abdominal aortic rings model. Concentration-response curves for mint oil were generated after precontracting isolated mouse aorta with phenylephrine. The effect of different receptor antagonists and ion channel or enzyme inhibitors on the vasorelaxant potential of mint oil were studied. Molecular docking studies were conducted using computational techniques to investigate the potential interactions between the bioactive constituents of mint oil and key vascular targets. The tension of aortic rings, which had been contracted by phenylephrine, relaxed as a function of the concentration of mint oil (0.0002–2 mg/mL). Pretreatment of the rings with the nitric oxide synthase inhibitor (L-NAME), a nonselective β-blocker (propranolol), and a muscarinic receptor blocker (atropine) didn’t show significant resistance to the vasodilatory effects of the mint oil. The vasodilatory effects of mint oil were significantly diminished when the rings were pretreated with glibenclamide, an inhibitor of ATP-sensitive K+ channels. In addition, indomethacin, a cyclooxygenase (COX) inhibitor, did influence mint oil’s tension in the preparations precontracted with phenylephrine. The present findings imply that ATP-sensitive K+ channels activation, blocking of Ca2+ channels, and inhibition of COX play a role in mediating the mint oil-induced vasorelaxation. Molecular docking studies of mint oil constituents showed that β-Elemene and Aromadendrene can interact with K+ and Ca2+ channels through various hydrophobic interactions with key amino acid residues. Additional work is needed to confirm the possible beneficial application of mint oil or its constituents in regulating the vascular tone. |
| first_indexed | 2025-11-15T14:43:35Z |
| format | Article |
| id | upm-119137 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:43:35Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1191372025-09-22T00:18:47Z http://psasir.upm.edu.my/id/eprint/119137/ The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study Alkharfy, Khalid M. Ahmad, Ajaz Almuaijel, Saleh Hashim, Abdullah Raish, Mohammad Jan, Basit L. Rehman, Najeeb Ur Anwar, Farooq Rehman, Md Tabish Alajmi, Mohamad F. The present study examined the vascular effects of peppermint or mint (Mentha longifolia L.) using an abdominal aortic rings model. Concentration-response curves for mint oil were generated after precontracting isolated mouse aorta with phenylephrine. The effect of different receptor antagonists and ion channel or enzyme inhibitors on the vasorelaxant potential of mint oil were studied. Molecular docking studies were conducted using computational techniques to investigate the potential interactions between the bioactive constituents of mint oil and key vascular targets. The tension of aortic rings, which had been contracted by phenylephrine, relaxed as a function of the concentration of mint oil (0.0002–2 mg/mL). Pretreatment of the rings with the nitric oxide synthase inhibitor (L-NAME), a nonselective β-blocker (propranolol), and a muscarinic receptor blocker (atropine) didn’t show significant resistance to the vasodilatory effects of the mint oil. The vasodilatory effects of mint oil were significantly diminished when the rings were pretreated with glibenclamide, an inhibitor of ATP-sensitive K+ channels. In addition, indomethacin, a cyclooxygenase (COX) inhibitor, did influence mint oil’s tension in the preparations precontracted with phenylephrine. The present findings imply that ATP-sensitive K+ channels activation, blocking of Ca2+ channels, and inhibition of COX play a role in mediating the mint oil-induced vasorelaxation. Molecular docking studies of mint oil constituents showed that β-Elemene and Aromadendrene can interact with K+ and Ca2+ channels through various hydrophobic interactions with key amino acid residues. Additional work is needed to confirm the possible beneficial application of mint oil or its constituents in regulating the vascular tone. Taylor and Francis 2024 Article PeerReviewed Alkharfy, Khalid M. and Ahmad, Ajaz and Almuaijel, Saleh and Hashim, Abdullah and Raish, Mohammad and Jan, Basit L. and Rehman, Najeeb Ur and Anwar, Farooq and Rehman, Md Tabish and Alajmi, Mohamad F. (2024) The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study. Journal of Biomolecular Structure and Dynamics. ISSN 0739-1102; eISSN: 1538-0254 https://www.tandfonline.com/doi/full/10.1080/07391102.2024.2439616 10.1080/07391102.2024.2439616 |
| spellingShingle | Alkharfy, Khalid M. Ahmad, Ajaz Almuaijel, Saleh Hashim, Abdullah Raish, Mohammad Jan, Basit L. Rehman, Najeeb Ur Anwar, Farooq Rehman, Md Tabish Alajmi, Mohamad F. The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title | The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title_full | The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title_fullStr | The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title_full_unstemmed | The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title_short | The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: an ex-vivo and computational study |
| title_sort | vascular effects of peppermint (mentha longifolia. l) on aorta in a mouse model: an ex-vivo and computational study |
| url | http://psasir.upm.edu.my/id/eprint/119137/ http://psasir.upm.edu.my/id/eprint/119137/ http://psasir.upm.edu.my/id/eprint/119137/ |