Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin

Streptozotocin (STZ) is widely used to increase blood glucose levels and generate diabetic animal models. However, the dose of STZ is important as it may lead to inadequate induction of diabetes, metabolic complications, and influence the behavior of animals. Therefore, this study aimed to determine...

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Main Authors: Titisari, Nurina, Fatimatuzzahra, Izza Nuruzzakiyah, Rahmawati, Nidya Putri, Adila, Nirmala Sekar, Fauzi, Ahmad, Razak, Intan Shameha Abdul, Samsulrizal, Nurdiana, Ahmad, Hafandi
Format: Article
Published: Research Journal of Pharmacy and Technology 2024
Online Access:http://psasir.upm.edu.my/id/eprint/118933/
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author Titisari, Nurina
Fatimatuzzahra, Izza Nuruzzakiyah
Rahmawati, Nidya Putri
Adila, Nirmala Sekar
Fauzi, Ahmad
Razak, Intan Shameha Abdul
Samsulrizal, Nurdiana
Ahmad, Hafandi
Ahmad, Hafandi
author_facet Titisari, Nurina
Fatimatuzzahra, Izza Nuruzzakiyah
Rahmawati, Nidya Putri
Adila, Nirmala Sekar
Fauzi, Ahmad
Razak, Intan Shameha Abdul
Samsulrizal, Nurdiana
Ahmad, Hafandi
Ahmad, Hafandi
author_sort Titisari, Nurina
building UPM Institutional Repository
collection Online Access
description Streptozotocin (STZ) is widely used to increase blood glucose levels and generate diabetic animal models. However, the dose of STZ is important as it may lead to inadequate induction of diabetes, metabolic complications, and influence the behavior of animals. Therefore, this study aimed to determine the various impacts of different STZ dosages on the brain cognitive performance associated with hyperglycemia and organ complications of diabetic rats. Animals were divided into three groups: (1) rats received a single dose of STZ (SSTZ; 55mg/kg), (2) rats received multiple doses of STZ (MSTZ; 40mg/kg) and (3) control rats received citrate buffer (CON; 0.2mL/rat) for three consecutive days intraperitoneally. Brain cognitive performance was assessed using the Y-maze test, and blood glucose level was performed weekly. The histopathological study was conducted on the pancreas, liver, kidney, and brain tissues. Results showed that animals with single and multiple doses of STZ decreased the number of entries and time spent in the novel arm of the Y-maze task. Multiple doses of STZ caused severe degenerative changes in the pancreatic islet, brain neuron apoptosis, inflammation in the liver, and tubular cell injuries. Thus, these results indicate that both single and multiple dosages of STZ influenced brain cognitive performance, which was associated with hyperglycemia and tissue degeneration in diabetic animals. © RJPT All right reserved.
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spelling upm-1189332025-07-30T01:46:43Z http://psasir.upm.edu.my/id/eprint/118933/ Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin Titisari, Nurina Fatimatuzzahra, Izza Nuruzzakiyah Rahmawati, Nidya Putri Adila, Nirmala Sekar Fauzi, Ahmad Razak, Intan Shameha Abdul Samsulrizal, Nurdiana Ahmad, Hafandi Ahmad, Hafandi Streptozotocin (STZ) is widely used to increase blood glucose levels and generate diabetic animal models. However, the dose of STZ is important as it may lead to inadequate induction of diabetes, metabolic complications, and influence the behavior of animals. Therefore, this study aimed to determine the various impacts of different STZ dosages on the brain cognitive performance associated with hyperglycemia and organ complications of diabetic rats. Animals were divided into three groups: (1) rats received a single dose of STZ (SSTZ; 55mg/kg), (2) rats received multiple doses of STZ (MSTZ; 40mg/kg) and (3) control rats received citrate buffer (CON; 0.2mL/rat) for three consecutive days intraperitoneally. Brain cognitive performance was assessed using the Y-maze test, and blood glucose level was performed weekly. The histopathological study was conducted on the pancreas, liver, kidney, and brain tissues. Results showed that animals with single and multiple doses of STZ decreased the number of entries and time spent in the novel arm of the Y-maze task. Multiple doses of STZ caused severe degenerative changes in the pancreatic islet, brain neuron apoptosis, inflammation in the liver, and tubular cell injuries. Thus, these results indicate that both single and multiple dosages of STZ influenced brain cognitive performance, which was associated with hyperglycemia and tissue degeneration in diabetic animals. © RJPT All right reserved. Research Journal of Pharmacy and Technology 2024 Article PeerReviewed Titisari, Nurina and Fatimatuzzahra, Izza Nuruzzakiyah and Rahmawati, Nidya Putri and Adila, Nirmala Sekar and Fauzi, Ahmad and Razak, Intan Shameha Abdul and Samsulrizal, Nurdiana and Ahmad, Hafandi and Ahmad, Hafandi (2024) Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin. Research Journal of Pharmacy and Technology, 17 (7). pp. 3381-3388. ISSN 0974-3618; eISSN: 0974-360X https://rjptonline.org/AbstractView.aspx?PID=2024-17-7-61 10.52711/0974-360x.2024.00528
spellingShingle Titisari, Nurina
Fatimatuzzahra, Izza Nuruzzakiyah
Rahmawati, Nidya Putri
Adila, Nirmala Sekar
Fauzi, Ahmad
Razak, Intan Shameha Abdul
Samsulrizal, Nurdiana
Ahmad, Hafandi
Ahmad, Hafandi
Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title_full Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title_fullStr Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title_full_unstemmed Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title_short Brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
title_sort brain cognitive performance and histopathological effects of diabetic rats induced by single and multiple dosages of streptozotocin
url http://psasir.upm.edu.my/id/eprint/118933/
http://psasir.upm.edu.my/id/eprint/118933/
http://psasir.upm.edu.my/id/eprint/118933/