Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption
The study focuses on enhancing the production and purification of 1,3-Propanediol (1,3-PDO), a versatile compound used across various industries. Conventionally, 1,3-propanediol (1,3-PDO) has been derived from petroleum-based feedstocks through chemical processes, which pose significant cost and env...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Institution of Chemical Engineers
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118858/ http://psasir.upm.edu.my/id/eprint/118858/1/118858.pdf |
| _version_ | 1848867806489083904 |
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| author | Ismail, Amer Aref Said Agustin, Yuana Elly Yeow, Teck Ann Baishan, Fang Jamali, Nur Syakina Yeap, Swee Keong Silvamany, Hemavathi Chee, Tan Peng Ping, Tan Jian |
| author_facet | Ismail, Amer Aref Said Agustin, Yuana Elly Yeow, Teck Ann Baishan, Fang Jamali, Nur Syakina Yeap, Swee Keong Silvamany, Hemavathi Chee, Tan Peng Ping, Tan Jian |
| author_sort | Ismail, Amer Aref Said |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The study focuses on enhancing the production and purification of 1,3-Propanediol (1,3-PDO), a versatile compound used across various industries. Conventionally, 1,3-propanediol (1,3-PDO) has been derived from petroleum-based feedstocks through chemical processes, which pose significant cost and environmental concerns. This has prompted the exploration of sustainable biological production methods using microorganisms to ferment glycerol, the main by-product of palm oil transesterification in biodiesel production. However, downstream purification remains complex and inefficient. To address this, our study introduces a novel and economically viable purification strategy for 1,3-PDO derived from a semi-industrial fermentation broth. The purification process involves pre-treatment steps: centrifugation, flocculation with chitosan, decolorization with activated charcoal, and broth concentration via rotary evaporation. These steps resulted in a transparent broth with a minimal 6 % loss of 1,3-PDO. Various resin types, including Sulfonated Styrene Divinylbenzene (S-SDVB) and Styrene Divinylbenzene (SDVB) ion exchange resins in different ionic forms in addition to silica gel resin, were evaluated for their effectiveness in separating 1,3-PDO in terms of recovery and purity. A key aspect of our study is the detailed investigation of the equilibrium adsorption characteristics of SDVB and S-SDVB resins, each with different ion forms. This analysis provides insights into how resin modifications affect the separation process, aiming for higher yields and purities of 1,3-PDO. The findings revealed that the Langmuir adsorption isotherm fits experimental data. Column chromatography experiments showed SDVB resins' deficiencies in separating 1,3-PDO from other components, while silica gel resin achieved a recovery rate exceeding 96 % purity and approximately 80 % overall recovery. Notably, the Ca2+ form of S-SDVB resin achieved high recovery (95–100 %) and purity (91–93 %) in both synthetic and real fermentation broths, addressing biodiesel challenges and proposing a solution for large-scale microbial 1,3-PDO production. |
| first_indexed | 2025-11-15T14:42:21Z |
| format | Article |
| id | upm-118858 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:42:21Z |
| publishDate | 2025 |
| publisher | Institution of Chemical Engineers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1188582025-07-28T03:00:35Z http://psasir.upm.edu.my/id/eprint/118858/ Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption Ismail, Amer Aref Said Agustin, Yuana Elly Yeow, Teck Ann Baishan, Fang Jamali, Nur Syakina Yeap, Swee Keong Silvamany, Hemavathi Chee, Tan Peng Ping, Tan Jian The study focuses on enhancing the production and purification of 1,3-Propanediol (1,3-PDO), a versatile compound used across various industries. Conventionally, 1,3-propanediol (1,3-PDO) has been derived from petroleum-based feedstocks through chemical processes, which pose significant cost and environmental concerns. This has prompted the exploration of sustainable biological production methods using microorganisms to ferment glycerol, the main by-product of palm oil transesterification in biodiesel production. However, downstream purification remains complex and inefficient. To address this, our study introduces a novel and economically viable purification strategy for 1,3-PDO derived from a semi-industrial fermentation broth. The purification process involves pre-treatment steps: centrifugation, flocculation with chitosan, decolorization with activated charcoal, and broth concentration via rotary evaporation. These steps resulted in a transparent broth with a minimal 6 % loss of 1,3-PDO. Various resin types, including Sulfonated Styrene Divinylbenzene (S-SDVB) and Styrene Divinylbenzene (SDVB) ion exchange resins in different ionic forms in addition to silica gel resin, were evaluated for their effectiveness in separating 1,3-PDO in terms of recovery and purity. A key aspect of our study is the detailed investigation of the equilibrium adsorption characteristics of SDVB and S-SDVB resins, each with different ion forms. This analysis provides insights into how resin modifications affect the separation process, aiming for higher yields and purities of 1,3-PDO. The findings revealed that the Langmuir adsorption isotherm fits experimental data. Column chromatography experiments showed SDVB resins' deficiencies in separating 1,3-PDO from other components, while silica gel resin achieved a recovery rate exceeding 96 % purity and approximately 80 % overall recovery. Notably, the Ca2+ form of S-SDVB resin achieved high recovery (95–100 %) and purity (91–93 %) in both synthetic and real fermentation broths, addressing biodiesel challenges and proposing a solution for large-scale microbial 1,3-PDO production. Institution of Chemical Engineers 2025-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118858/1/118858.pdf Ismail, Amer Aref Said and Agustin, Yuana Elly and Yeow, Teck Ann and Baishan, Fang and Jamali, Nur Syakina and Yeap, Swee Keong and Silvamany, Hemavathi and Chee, Tan Peng and Ping, Tan Jian (2025) Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption. Food and Bioproducts Processing, 149. pp. 100-111. ISSN 0960-3085; eISSN: 0960-3085 https://linkinghub.elsevier.com/retrieve/pii/S0960308524002268 10.1016/j.fbp.2024.11.004 |
| spellingShingle | Ismail, Amer Aref Said Agustin, Yuana Elly Yeow, Teck Ann Baishan, Fang Jamali, Nur Syakina Yeap, Swee Keong Silvamany, Hemavathi Chee, Tan Peng Ping, Tan Jian Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title | Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title_full | Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title_fullStr | Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title_full_unstemmed | Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title_short | Sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| title_sort | sustainable biological production and innovative purification of the 1,3-propanediol from glycerol fermentation broth via resin adsorption |
| url | http://psasir.upm.edu.my/id/eprint/118858/ http://psasir.upm.edu.my/id/eprint/118858/ http://psasir.upm.edu.my/id/eprint/118858/ http://psasir.upm.edu.my/id/eprint/118858/1/118858.pdf |