Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics
Colorectal cancer's escalating prevalence in Malaysia prompts the exploration of innovative anticancer agents; amidst this backdrop, tricyclohexyltin p-methoxycinnamate emerges as a synthesized organotin complex with unique bioactive properties. Notably, the novelty of this research lies in its...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118697/ http://psasir.upm.edu.my/id/eprint/118697/1/118697.pdf |
| _version_ | 1848867576481841152 |
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| author | Md Akim, Abdah Chaudhry, Gul-e-Saba Zeenia Ken, Tan Weay Sung, Yeong Yik Tengku Muhammad, Tengku Sifzizul |
| author_facet | Md Akim, Abdah Chaudhry, Gul-e-Saba Zeenia Ken, Tan Weay Sung, Yeong Yik Tengku Muhammad, Tengku Sifzizul |
| author_sort | Md Akim, Abdah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Colorectal cancer's escalating prevalence in Malaysia prompts the exploration of innovative anticancer agents; amidst this backdrop, tricyclohexyltin p-methoxycinnamate emerges as a synthesized organotin complex with unique bioactive properties. Notably, the novelty of this research lies in its groundbreaking investigation into the hitherto unexplored anticancer potential and mode of cell death induced by tricyclohexyltin p-methoxycinnamate on colon cancer (human colorectal adenocarcinoma cell line (HT-29)) cell lines. This study pioneers the assessment of tricyclohexyltin p-methoxycinnamate's cytotoxic effects through the '(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay,' revealing a dose- and time-dependent cytotoxicity with IC 50 values of 1.2 × 10-6M for 24 h, 1.0 × 10-6M for 48 h, and 5.0 × 10-7M for 72 h. The mode of cell death through 'AO/PI' staining alongside cell cycle analysis, highlighting apoptosis as the predominant mode of cell death in the HT-29 cell line, accompanied by substantial cell cycle arrest at the sub-'G0' phase. The tricyclohexyltin p-methoxycinnamate's shown potential antiproliferative properties, cell cycle arrest, and apoptosis in HT-29 cancer cells. This novel insight into the compound's mode of action positions it as a promising candidate for future anticancer therapeutics. The study underscores the urgency of investigating innovative approaches amidst the rising colorectal cancer rates, emphasizing the compound's potential through further in-depth studies and preclinical trials. |
| first_indexed | 2025-11-15T14:38:42Z |
| format | Article |
| id | upm-118697 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:38:42Z |
| publishDate | 2025 |
| publisher | Wolters Kluwer Medknow Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1186972025-07-22T04:02:05Z http://psasir.upm.edu.my/id/eprint/118697/ Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics Md Akim, Abdah Chaudhry, Gul-e-Saba Zeenia Ken, Tan Weay Sung, Yeong Yik Tengku Muhammad, Tengku Sifzizul Colorectal cancer's escalating prevalence in Malaysia prompts the exploration of innovative anticancer agents; amidst this backdrop, tricyclohexyltin p-methoxycinnamate emerges as a synthesized organotin complex with unique bioactive properties. Notably, the novelty of this research lies in its groundbreaking investigation into the hitherto unexplored anticancer potential and mode of cell death induced by tricyclohexyltin p-methoxycinnamate on colon cancer (human colorectal adenocarcinoma cell line (HT-29)) cell lines. This study pioneers the assessment of tricyclohexyltin p-methoxycinnamate's cytotoxic effects through the '(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay,' revealing a dose- and time-dependent cytotoxicity with IC 50 values of 1.2 × 10-6M for 24 h, 1.0 × 10-6M for 48 h, and 5.0 × 10-7M for 72 h. The mode of cell death through 'AO/PI' staining alongside cell cycle analysis, highlighting apoptosis as the predominant mode of cell death in the HT-29 cell line, accompanied by substantial cell cycle arrest at the sub-'G0' phase. The tricyclohexyltin p-methoxycinnamate's shown potential antiproliferative properties, cell cycle arrest, and apoptosis in HT-29 cancer cells. This novel insight into the compound's mode of action positions it as a promising candidate for future anticancer therapeutics. The study underscores the urgency of investigating innovative approaches amidst the rising colorectal cancer rates, emphasizing the compound's potential through further in-depth studies and preclinical trials. Wolters Kluwer Medknow Publications 2025 Article PeerReviewed text en cc_by_nc_sa_4 http://psasir.upm.edu.my/id/eprint/118697/1/118697.pdf Md Akim, Abdah and Chaudhry, Gul-e-Saba and Zeenia and Ken, Tan Weay and Sung, Yeong Yik and Tengku Muhammad, Tengku Sifzizul (2025) Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics. Journal of Advanced Pharmaceutical Technology and Research, 16 (1). pp. 1-5. ISSN 2231-4040; eISSN: 0976-2094 https://journals.lww.com/japtr/fulltext/2025/01000/cytotoxic_effect_and_apoptotic_induction_of.1.aspx 10.4103/JAPTR.JAPTR_427_23 |
| spellingShingle | Md Akim, Abdah Chaudhry, Gul-e-Saba Zeenia Ken, Tan Weay Sung, Yeong Yik Tengku Muhammad, Tengku Sifzizul Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title | Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title_full | Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title_fullStr | Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title_full_unstemmed | Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title_short | Cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on HT-29 colorectal cancer cells: Implications for anticancer therapeutics |
| title_sort | cytotoxic effect and apoptotic induction of tricyclohexyltin p-methoxycinnamate on ht-29 colorectal cancer cells: implications for anticancer therapeutics |
| url | http://psasir.upm.edu.my/id/eprint/118697/ http://psasir.upm.edu.my/id/eprint/118697/ http://psasir.upm.edu.my/id/eprint/118697/ http://psasir.upm.edu.my/id/eprint/118697/1/118697.pdf |