The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings
An electrospun chitosan-gelatin (CS-GL) nanofiber encapsulated with the curcumin (CUR)-chrysin (CHR)-loaded copper (Cu) metal-organic framework (MOF) was successfully synthesized in this study. A high percentage of encapsulation efficiency (99.2%) of CUR and CHR in Cu-MOF-CUR-CHR was yielded. Other...
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| Format: | Article |
| Language: | English |
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American Chemical Society
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118695/ http://psasir.upm.edu.my/id/eprint/118695/1/118695.pdf |
| _version_ | 1848867575977476096 |
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| author | Soo, Ghee Yeoh Yun, Khoon Liew Wei, Meng Lim Abdul Rahman, Norizah Yoon, Yee Then |
| author_facet | Soo, Ghee Yeoh Yun, Khoon Liew Wei, Meng Lim Abdul Rahman, Norizah Yoon, Yee Then |
| author_sort | Soo, Ghee Yeoh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An electrospun chitosan-gelatin (CS-GL) nanofiber encapsulated with the curcumin (CUR)-chrysin (CHR)-loaded copper (Cu) metal-organic framework (MOF) was successfully synthesized in this study. A high percentage of encapsulation efficiency (99.2%) of CUR and CHR in Cu-MOF-CUR-CHR was yielded. Other than the confirmation of the successful fusion of MOFs with CUR-CHR in CS-GL/Cu-MOF-CUR-CHR nanofibers using Fourier-transform infrared (FTIR) spectroscopy, the scanning electron microscopy (SEM) images depicted an average diameter in the range of 302-380 nm. The percentage of cumulative drug release of CS-GL/20% Cu-MOF-CUR-CHR nanofibers in phosphate-buffered solution at pH 7.4 after 48 h was 98.9%, which was higher than CS-GL/CUR-CHR nanofibers without Cu-MOFs. In the cytotoxicity test on Human epidermal keratinocytes cell line cells, it was noticed that 1% and 5% CS-GL/Cu-MOF-CUR-CHR nanofibers exhibited high cell survival (>90%). Through the colony counting method, the logarithm reduction values (LRVs) of CS-GL/5% Cu-MOF-CUR-CHR nanofibers were 2.69 against Staphylococcus aureus and 1.87 against Pseudomonas aeruginosa. It was noteworthy that the CS-GL/Cu-MOF-CUR-CHR nanofiber exhibited a higher antibacterial activity against Gram-positive than Gram-negative bacteria. Overall, the biocompatible CS-GL/Cu-MOF-CUR-CHR nanofibers with an effective antibacterial effect have the potential to be used as an alternative to antibiotics in antibacterial wound dressing applications. |
| first_indexed | 2025-11-15T14:38:41Z |
| format | Article |
| id | upm-118695 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:38:41Z |
| publishDate | 2025 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1186952025-07-22T03:49:11Z http://psasir.upm.edu.my/id/eprint/118695/ The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings Soo, Ghee Yeoh Yun, Khoon Liew Wei, Meng Lim Abdul Rahman, Norizah Yoon, Yee Then An electrospun chitosan-gelatin (CS-GL) nanofiber encapsulated with the curcumin (CUR)-chrysin (CHR)-loaded copper (Cu) metal-organic framework (MOF) was successfully synthesized in this study. A high percentage of encapsulation efficiency (99.2%) of CUR and CHR in Cu-MOF-CUR-CHR was yielded. Other than the confirmation of the successful fusion of MOFs with CUR-CHR in CS-GL/Cu-MOF-CUR-CHR nanofibers using Fourier-transform infrared (FTIR) spectroscopy, the scanning electron microscopy (SEM) images depicted an average diameter in the range of 302-380 nm. The percentage of cumulative drug release of CS-GL/20% Cu-MOF-CUR-CHR nanofibers in phosphate-buffered solution at pH 7.4 after 48 h was 98.9%, which was higher than CS-GL/CUR-CHR nanofibers without Cu-MOFs. In the cytotoxicity test on Human epidermal keratinocytes cell line cells, it was noticed that 1% and 5% CS-GL/Cu-MOF-CUR-CHR nanofibers exhibited high cell survival (>90%). Through the colony counting method, the logarithm reduction values (LRVs) of CS-GL/5% Cu-MOF-CUR-CHR nanofibers were 2.69 against Staphylococcus aureus and 1.87 against Pseudomonas aeruginosa. It was noteworthy that the CS-GL/Cu-MOF-CUR-CHR nanofiber exhibited a higher antibacterial activity against Gram-positive than Gram-negative bacteria. Overall, the biocompatible CS-GL/Cu-MOF-CUR-CHR nanofibers with an effective antibacterial effect have the potential to be used as an alternative to antibiotics in antibacterial wound dressing applications. American Chemical Society 2025 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/118695/1/118695.pdf Soo, Ghee Yeoh and Yun, Khoon Liew and Wei, Meng Lim and Abdul Rahman, Norizah and Yoon, Yee Then (2025) The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings. ACS Omega, 10 (21). pp. 21065-21076. ISSN 2470-1343 https://pubs.acs.org/doi/10.1021/acsomega.4c08100 10.1021/acsomega.4c08100 |
| spellingShingle | Soo, Ghee Yeoh Yun, Khoon Liew Wei, Meng Lim Abdul Rahman, Norizah Yoon, Yee Then The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title | The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title_full | The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title_fullStr | The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title_full_unstemmed | The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title_short | The preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| title_sort | preparation and characterization of electrospun chitosan-gelatin nanofibers containing copper-metal-organic frameworks loaded with curcumin and chrysin as antibacterial wound dressings |
| url | http://psasir.upm.edu.my/id/eprint/118695/ http://psasir.upm.edu.my/id/eprint/118695/ http://psasir.upm.edu.my/id/eprint/118695/ http://psasir.upm.edu.my/id/eprint/118695/1/118695.pdf |