Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement
The CRISPR/Cas9 genome editing system has been in the spotlight compared to programmable nucleases such as ZFNs and TALENs due to its simplicity, versatility, and high efficiency. CRISPR/Cas9 has revolutionized plant genetic engineering and is broadly used to edit various plants' genomes, inclu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118564/ http://psasir.upm.edu.my/id/eprint/118564/1/118564.pdf |
| _version_ | 1848867765160509440 |
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| author | Shaharuddin, Noor Azmi Mat Yunus, Abdul Masani Norfaezah, Jamaludin Bohari, Bahariah Md Piji, Mohd Al-Akmarul Fizree Wan Sulaiman, Wan Nur Syuhada Abdul Rasid, Omar Ghulam Kadir, Ahmad Parveez |
| author_facet | Shaharuddin, Noor Azmi Mat Yunus, Abdul Masani Norfaezah, Jamaludin Bohari, Bahariah Md Piji, Mohd Al-Akmarul Fizree Wan Sulaiman, Wan Nur Syuhada Abdul Rasid, Omar Ghulam Kadir, Ahmad Parveez |
| author_sort | Shaharuddin, Noor Azmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The CRISPR/Cas9 genome editing system has been in the spotlight compared to programmable nucleases such as ZFNs and TALENs due to its simplicity, versatility, and high efficiency. CRISPR/Cas9 has revolutionized plant genetic engineering and is broadly used to edit various plants' genomes, including those transformation-recalcitrant species such as oil palm. This review will comprehensively present the CRISPR-Cas9 system's brief history and underlying mechanisms. We then highlighted the establishment of the CRISPR/Cas9 system in plants with an emphasis on the strategies of highly efficient guide RNA design, the establishment of various CRISPR/Cas9 vector systems, approaches of multiplex editing, methods of transformation for stable and transient techniques, available methods for detecting and analyzing mutations, which have been applied and could be adopted for CRISPR/Cas9 genome editing in oil palm. In addition, we also provide insight into the strategy of DNA-free genome editing and its potential application in oil palm. |
| first_indexed | 2025-11-15T14:41:42Z |
| format | Article |
| id | upm-118564 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:41:42Z |
| publishDate | 2024 |
| publisher | SpringerOpen |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1185642025-08-05T04:52:22Z http://psasir.upm.edu.my/id/eprint/118564/ Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement Shaharuddin, Noor Azmi Mat Yunus, Abdul Masani Norfaezah, Jamaludin Bohari, Bahariah Md Piji, Mohd Al-Akmarul Fizree Wan Sulaiman, Wan Nur Syuhada Abdul Rasid, Omar Ghulam Kadir, Ahmad Parveez The CRISPR/Cas9 genome editing system has been in the spotlight compared to programmable nucleases such as ZFNs and TALENs due to its simplicity, versatility, and high efficiency. CRISPR/Cas9 has revolutionized plant genetic engineering and is broadly used to edit various plants' genomes, including those transformation-recalcitrant species such as oil palm. This review will comprehensively present the CRISPR-Cas9 system's brief history and underlying mechanisms. We then highlighted the establishment of the CRISPR/Cas9 system in plants with an emphasis on the strategies of highly efficient guide RNA design, the establishment of various CRISPR/Cas9 vector systems, approaches of multiplex editing, methods of transformation for stable and transient techniques, available methods for detecting and analyzing mutations, which have been applied and could be adopted for CRISPR/Cas9 genome editing in oil palm. In addition, we also provide insight into the strategy of DNA-free genome editing and its potential application in oil palm. SpringerOpen 2024-05-28 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118564/1/118564.pdf Shaharuddin, Noor Azmi and Mat Yunus, Abdul Masani and Norfaezah, Jamaludin and Bohari, Bahariah and Md Piji, Mohd Al-Akmarul Fizree and Wan Sulaiman, Wan Nur Syuhada and Abdul Rasid, Omar and Ghulam Kadir, Ahmad Parveez (2024) Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement. 3 Biotech, 14 (6). pp. 1-20. ISSN 2190-5738; eISSN: 2190-572X https://link.springer.com/article/10.1007/s13205-024-04010-w 10.1007/s13205-024-04010-w |
| spellingShingle | Shaharuddin, Noor Azmi Mat Yunus, Abdul Masani Norfaezah, Jamaludin Bohari, Bahariah Md Piji, Mohd Al-Akmarul Fizree Wan Sulaiman, Wan Nur Syuhada Abdul Rasid, Omar Ghulam Kadir, Ahmad Parveez Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title | Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title_full | Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title_fullStr | Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title_full_unstemmed | Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title_short | Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement |
| title_sort | towards dna‑free crispr/cas9 genome editing for sustainable oil palm improvement |
| url | http://psasir.upm.edu.my/id/eprint/118564/ http://psasir.upm.edu.my/id/eprint/118564/ http://psasir.upm.edu.my/id/eprint/118564/ http://psasir.upm.edu.my/id/eprint/118564/1/118564.pdf |