Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters
The bioconversion of dicoumarol by Aspergillus fumigatus NRRL 163 using methanolic extract of Gliricidia sepium leaves was thoroughly investigated, special emphasis on the influence of medium composition and fermentation conditions. The ideal conditions were determined by utilizing a defined medium...
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Elsevier
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118506/ http://psasir.upm.edu.my/id/eprint/118506/1/118506.pdf |
| _version_ | 1848867531038654464 |
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| author | Yaacob, Siti Zalina Abdullah, Luqman Chuah Lee, Chuan Li Abdullah, Nor Hafizah Chin, Kit Ling |
| author_facet | Yaacob, Siti Zalina Abdullah, Luqman Chuah Lee, Chuan Li Abdullah, Nor Hafizah Chin, Kit Ling |
| author_sort | Yaacob, Siti Zalina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The bioconversion of dicoumarol by Aspergillus fumigatus NRRL 163 using methanolic extract of Gliricidia sepium leaves was thoroughly investigated, special emphasis on the influence of medium composition and fermentation conditions. The ideal conditions were determined by utilizing a defined medium containing o-coumaric acid extracted from G.sepium leaves. This resulted in the highest dicoumarol productivity (153.81 U mL h-1), cell efficiency (0.027 g L cell-1), and overall dicoumarol productivity (4.15 U mL⋅h-1). The Monod kinetic model effectively described the relationship between growth rate, substrate concentration, and biomass production. With over 95 % confidence, the model showed no significant difference between calculated and observed values, confirming its accuracy in predicting microbial growth, substrate utilization, and dicoumarol biosynthesis. This approach enhanced dicoumarol productivity (167.92 U mL⁻¹ h⁻¹) and cell efficiency (37.13 g mL⁻¹ cells⁻¹). Furthermore, an extensive quantitative analysis utilizing HPLC, FTIR, LCMS/MS and vitro rodenticides (aPTT and PT test) was conducted, revealing substantial anticoagulant activity of dicoumarol, notably in inhibiting intrinsic pathways of coagulation. The successful identification of dicoumarol through LCMS/MS and the confirmation of its structure (C19H12O6) via FT-IR spectra highlighted the robustness of the approach. Moreover, the dicoumarol fraction's ability to double coagulation time in the aPTT test underscored its potent anticoagulant properties. These findings offer novel insights into dicoumarol bioconversion and its pharmacological significance in rodenticide anticoagulant activity. |
| first_indexed | 2025-11-15T14:37:58Z |
| format | Article |
| id | upm-118506 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:37:58Z |
| publishDate | 2025 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1185062025-07-17T03:46:33Z http://psasir.upm.edu.my/id/eprint/118506/ Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters Yaacob, Siti Zalina Abdullah, Luqman Chuah Lee, Chuan Li Abdullah, Nor Hafizah Chin, Kit Ling The bioconversion of dicoumarol by Aspergillus fumigatus NRRL 163 using methanolic extract of Gliricidia sepium leaves was thoroughly investigated, special emphasis on the influence of medium composition and fermentation conditions. The ideal conditions were determined by utilizing a defined medium containing o-coumaric acid extracted from G.sepium leaves. This resulted in the highest dicoumarol productivity (153.81 U mL h-1), cell efficiency (0.027 g L cell-1), and overall dicoumarol productivity (4.15 U mL⋅h-1). The Monod kinetic model effectively described the relationship between growth rate, substrate concentration, and biomass production. With over 95 % confidence, the model showed no significant difference between calculated and observed values, confirming its accuracy in predicting microbial growth, substrate utilization, and dicoumarol biosynthesis. This approach enhanced dicoumarol productivity (167.92 U mL⁻¹ h⁻¹) and cell efficiency (37.13 g mL⁻¹ cells⁻¹). Furthermore, an extensive quantitative analysis utilizing HPLC, FTIR, LCMS/MS and vitro rodenticides (aPTT and PT test) was conducted, revealing substantial anticoagulant activity of dicoumarol, notably in inhibiting intrinsic pathways of coagulation. The successful identification of dicoumarol through LCMS/MS and the confirmation of its structure (C19H12O6) via FT-IR spectra highlighted the robustness of the approach. Moreover, the dicoumarol fraction's ability to double coagulation time in the aPTT test underscored its potent anticoagulant properties. These findings offer novel insights into dicoumarol bioconversion and its pharmacological significance in rodenticide anticoagulant activity. Elsevier 2025-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118506/1/118506.pdf Yaacob, Siti Zalina and Abdullah, Luqman Chuah and Lee, Chuan Li and Abdullah, Nor Hafizah and Chin, Kit Ling (2025) Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters. Results in Engineering, 26. art. no. 105008. pp. 1-11. ISSN 2590-1230; https://www.sciencedirect.com/science/article/pii/S2590123025010837 Chemical engineering - Research Biochemical engineering Natural products - Synthesis 10.1016/j.rineng.2025.105008 |
| spellingShingle | Chemical engineering - Research Biochemical engineering Natural products - Synthesis Yaacob, Siti Zalina Abdullah, Luqman Chuah Lee, Chuan Li Abdullah, Nor Hafizah Chin, Kit Ling Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title | Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title_full | Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title_fullStr | Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title_full_unstemmed | Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title_short | Enhancement of dicoumarol production by Aspergillus fumigatus using methanolic extract of Gliricidia sepium leaves: Insights into fermentation kinetics parameters |
| title_sort | enhancement of dicoumarol production by aspergillus fumigatus using methanolic extract of gliricidia sepium leaves: insights into fermentation kinetics parameters |
| topic | Chemical engineering - Research Biochemical engineering Natural products - Synthesis |
| url | http://psasir.upm.edu.my/id/eprint/118506/ http://psasir.upm.edu.my/id/eprint/118506/ http://psasir.upm.edu.my/id/eprint/118506/ http://psasir.upm.edu.my/id/eprint/118506/1/118506.pdf |