Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system
Plasticizer pollution of the water environment is one of the world's most serious environmental issues. Phthalate plasticizers can disrupt endocrine function in vertebrates. Therefore, this study analyzed thyroid-related, reproduction-related, and estrogenresponsive genes in Japanese medaka (Or...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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John Wiley and Sons
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118432/ http://psasir.upm.edu.my/id/eprint/118432/1/118432.pdf |
| _version_ | 1848867758818721792 |
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| author | Horie, Yoshifumi Ramaswamy, Babu Rajendran Ríos, Juan Manuel Chee, Kong Yap Okamura, Hideo |
| author_facet | Horie, Yoshifumi Ramaswamy, Babu Rajendran Ríos, Juan Manuel Chee, Kong Yap Okamura, Hideo |
| author_sort | Horie, Yoshifumi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Plasticizer pollution of the water environment is one of the world's most serious environmental issues. Phthalate plasticizers can disrupt endocrine function in vertebrates. Therefore, this study analyzed thyroid-related, reproduction-related, and estrogenresponsive genes in Japanese medaka (Oryzias latipes) to determine whether nonphthalate diisobutyl adipate (DIBA) plasticizer could affect endocrine hormone activity or not. Developmental toxicity during fish embryogenesis was also evaluated. At a concentration of 11.57 mg/l, embryonic exposure to DIBA increased the mortality rate. Although abnormal development, including body curvature, edema, and lack of swim bladder inflation, was observed at 3.54 and 11.57 mg/l DIBA, growth inhibition and reduced swimming performance were also observed. In addition, DIBA exposure increased the levels of thyroid-stimulating hormone beta-subunit (tshβ) and deiodinase 1(dio1) but decreased the levels of thyroid hormone receptor alpha (trα) and beta (trβ). These results suggest that DIBA has thyroid hormone-disrupting activities in fish. However, kisspeptin (kiss1 and kiss2), gonadotropin-releasing hormone (gnrh1), folliclestimulating hormone beta (fshβ), luteinizing hormone beta (lhβ), choriogenin H (chgH), and vitellogenin (vtg1) expression did not change dose-dependently in response to DIBA exposure, whereas gnrh2 and vtg2 expression was elevated. These results indicate that DIBA has low estrogenic activity and does not disrupt the endocrine reproduction system in fish. Overall, this is the first report indicating that non-phthalate DIBA plasticizer is embryotoxic and disrupt thyroid hormone activity in fish. |
| first_indexed | 2025-11-15T14:41:35Z |
| format | Article |
| id | upm-118432 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:41:35Z |
| publishDate | 2023 |
| publisher | John Wiley and Sons |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1184322025-08-05T08:39:35Z http://psasir.upm.edu.my/id/eprint/118432/ Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system Horie, Yoshifumi Ramaswamy, Babu Rajendran Ríos, Juan Manuel Chee, Kong Yap Okamura, Hideo Plasticizer pollution of the water environment is one of the world's most serious environmental issues. Phthalate plasticizers can disrupt endocrine function in vertebrates. Therefore, this study analyzed thyroid-related, reproduction-related, and estrogenresponsive genes in Japanese medaka (Oryzias latipes) to determine whether nonphthalate diisobutyl adipate (DIBA) plasticizer could affect endocrine hormone activity or not. Developmental toxicity during fish embryogenesis was also evaluated. At a concentration of 11.57 mg/l, embryonic exposure to DIBA increased the mortality rate. Although abnormal development, including body curvature, edema, and lack of swim bladder inflation, was observed at 3.54 and 11.57 mg/l DIBA, growth inhibition and reduced swimming performance were also observed. In addition, DIBA exposure increased the levels of thyroid-stimulating hormone beta-subunit (tshβ) and deiodinase 1(dio1) but decreased the levels of thyroid hormone receptor alpha (trα) and beta (trβ). These results suggest that DIBA has thyroid hormone-disrupting activities in fish. However, kisspeptin (kiss1 and kiss2), gonadotropin-releasing hormone (gnrh1), folliclestimulating hormone beta (fshβ), luteinizing hormone beta (lhβ), choriogenin H (chgH), and vitellogenin (vtg1) expression did not change dose-dependently in response to DIBA exposure, whereas gnrh2 and vtg2 expression was elevated. These results indicate that DIBA has low estrogenic activity and does not disrupt the endocrine reproduction system in fish. Overall, this is the first report indicating that non-phthalate DIBA plasticizer is embryotoxic and disrupt thyroid hormone activity in fish. John Wiley and Sons 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118432/1/118432.pdf Horie, Yoshifumi and Ramaswamy, Babu Rajendran and Ríos, Juan Manuel and Chee, Kong Yap and Okamura, Hideo (2023) Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system. Journal of Applied Toxicology, 43 (7). pp. 982-992. ISSN 0260-437X; eISSN: 1099-1263 https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jat.4437 10.1002/jat.4437 |
| spellingShingle | Horie, Yoshifumi Ramaswamy, Babu Rajendran Ríos, Juan Manuel Chee, Kong Yap Okamura, Hideo Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title | Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title_full | Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title_fullStr | Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title_full_unstemmed | Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title_short | Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system |
| title_sort | effects of plasticizer diisobutyl adipate on the japanese medaka (oryzias latipes) endocrine system |
| url | http://psasir.upm.edu.my/id/eprint/118432/ http://psasir.upm.edu.my/id/eprint/118432/ http://psasir.upm.edu.my/id/eprint/118432/ http://psasir.upm.edu.my/id/eprint/118432/1/118432.pdf |