Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender
The widespread use of antibiotics contributes to the development of antibiotic resistance. Therefore, exploring suitable alternatives to antibiotics is crucial. This study aimed to determine the antibacterial effect of mulberry, roselle and aloe vera extracts on bacteria isolated from buck semen and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118312/ http://psasir.upm.edu.my/id/eprint/118312/1/118312.pdf |
| _version_ | 1848867486093541376 |
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| author | Shikh Maidin, Mashitah Mohammed, Nur Hafizah I., Siti Syamsiah M., Noor Izza M. I., Norzuraiti |
| author_facet | Shikh Maidin, Mashitah Mohammed, Nur Hafizah I., Siti Syamsiah M., Noor Izza M. I., Norzuraiti |
| author_sort | Shikh Maidin, Mashitah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The widespread use of antibiotics contributes to the development of antibiotic resistance. Therefore, exploring suitable alternatives to antibiotics is crucial. This study aimed to determine the antibacterial effect of mulberry, roselle and aloe vera extracts on bacteria isolated from buck semen and their effectiveness as an alternative to antibiotics for semen extender. The antibacterial activity of plant extract against identified bacteria was determined by using disc diffusion method. Semen was added to the extender with mulberry, roselle or aloe vera extract and incubated at 37 °C for 3 h and cryopreserved for 24 h before the assessment of bacterial load and semen quality were investigated. Results shows that mulberry extract was the only plant extract that significantly inhibited both gram-negative and gram-positive bacteria, with greater inhibitory zone on gram positive bacteria (22.33 ± 1.12 mm) compare to Roselle (8.1 ± 1.08 mm) and Aloe vera (9.6 ± 1.05 mm) resulting in the lowest bacterial load (763 ± 4.57 CFU/ml) and improved sperm quality during the 3-hour incubation period. Whereas, aloe vera and roselle extracts were highly effective for decreasing bacterial contamination and protecting the sperm quality of post-thawed semen. The study concluded that, mulberry extract was a suitable alternative to commercial antibiotics for fresh semen extenders, while aloe vera and roselle were more recommended for cryopreservation purposes. |
| first_indexed | 2025-11-15T14:37:15Z |
| format | Article |
| id | upm-118312 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:37:15Z |
| publishDate | 2024 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1183122025-07-08T08:49:46Z http://psasir.upm.edu.my/id/eprint/118312/ Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender Shikh Maidin, Mashitah Mohammed, Nur Hafizah I., Siti Syamsiah M., Noor Izza M. I., Norzuraiti The widespread use of antibiotics contributes to the development of antibiotic resistance. Therefore, exploring suitable alternatives to antibiotics is crucial. This study aimed to determine the antibacterial effect of mulberry, roselle and aloe vera extracts on bacteria isolated from buck semen and their effectiveness as an alternative to antibiotics for semen extender. The antibacterial activity of plant extract against identified bacteria was determined by using disc diffusion method. Semen was added to the extender with mulberry, roselle or aloe vera extract and incubated at 37 °C for 3 h and cryopreserved for 24 h before the assessment of bacterial load and semen quality were investigated. Results shows that mulberry extract was the only plant extract that significantly inhibited both gram-negative and gram-positive bacteria, with greater inhibitory zone on gram positive bacteria (22.33 ± 1.12 mm) compare to Roselle (8.1 ± 1.08 mm) and Aloe vera (9.6 ± 1.05 mm) resulting in the lowest bacterial load (763 ± 4.57 CFU/ml) and improved sperm quality during the 3-hour incubation period. Whereas, aloe vera and roselle extracts were highly effective for decreasing bacterial contamination and protecting the sperm quality of post-thawed semen. The study concluded that, mulberry extract was a suitable alternative to commercial antibiotics for fresh semen extenders, while aloe vera and roselle were more recommended for cryopreservation purposes. Malaysian Agricultural Research and Development Institute (MARDI) 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118312/1/118312.pdf Shikh Maidin, Mashitah and Mohammed, Nur Hafizah and I., Siti Syamsiah and M., Noor Izza and M. I., Norzuraiti (2024) Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender. Journal of Tropical Agriculture and Food Science, 52 (2). pp. 49-58. ISSN 1394-9829; eISSN: 2685‑3604 http://jtafs.mardi.gov.my/index.php/publication/jtafs-issues/issues/200-2024/vol-52-no-2/371-plant-extracts-as-an-alternative-for-antibiotics-in-saanen-goats-semen-extender |
| spellingShingle | Shikh Maidin, Mashitah Mohammed, Nur Hafizah I., Siti Syamsiah M., Noor Izza M. I., Norzuraiti Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title | Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title_full | Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title_fullStr | Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title_full_unstemmed | Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title_short | Plant extracts as an alternative for antibiotics in Saanen goats’ semen extender |
| title_sort | plant extracts as an alternative for antibiotics in saanen goats’ semen extender |
| url | http://psasir.upm.edu.my/id/eprint/118312/ http://psasir.upm.edu.my/id/eprint/118312/ http://psasir.upm.edu.my/id/eprint/118312/1/118312.pdf |