Pineapple peel bromelain extract improves poultry in vitro protein digestibility
This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society of Animal Production
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118225/ http://psasir.upm.edu.my/id/eprint/118225/1/118225.pdf |
| _version_ | 1848867463901478912 |
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| author | Akit, Henny Loh, Teck Chwen Ab Aziz, Muhamad Faris Foo, Hooi Ling Zailani, N. S. |
| author_facet | Akit, Henny Loh, Teck Chwen Ab Aziz, Muhamad Faris Foo, Hooi Ling Zailani, N. S. |
| author_sort | Akit, Henny |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone and in combination with 0.02 M dithiothreitol (DTT), 0.02% sodium azide, and 0.02 M ethylenediaminetetraacetic acid (EDTA). Subsequently, the effects of bromelain extracts on protein solubilization and digestibility using a poultry gut simulation model were evaluated. The findings indicated that bromelain extractions using deionized water (82.22 U/mg) and a combination of deionized water + sodium azide + EDTA (87.84 U/mg) exhibited the highest proteolytic activities compared to other extractants (P<0.05). Furthermore, pineapple peel bromelain extracts resulted in up to 24% increase in protein solubility and up to 6% increase in protein digestibility (P<0.05, respectively). Notably, bromelain extracted using deionized water (60.69 U/mg) proved to be the most effective in digesting protein in vitro. |
| first_indexed | 2025-11-15T14:36:54Z |
| format | Article |
| id | upm-118225 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:36:54Z |
| publishDate | 2024 |
| publisher | Malaysian Society of Animal Production |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1182252025-07-01T06:54:07Z http://psasir.upm.edu.my/id/eprint/118225/ Pineapple peel bromelain extract improves poultry in vitro protein digestibility Akit, Henny Loh, Teck Chwen Ab Aziz, Muhamad Faris Foo, Hooi Ling Zailani, N. S. This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone and in combination with 0.02 M dithiothreitol (DTT), 0.02% sodium azide, and 0.02 M ethylenediaminetetraacetic acid (EDTA). Subsequently, the effects of bromelain extracts on protein solubilization and digestibility using a poultry gut simulation model were evaluated. The findings indicated that bromelain extractions using deionized water (82.22 U/mg) and a combination of deionized water + sodium azide + EDTA (87.84 U/mg) exhibited the highest proteolytic activities compared to other extractants (P<0.05). Furthermore, pineapple peel bromelain extracts resulted in up to 24% increase in protein solubility and up to 6% increase in protein digestibility (P<0.05, respectively). Notably, bromelain extracted using deionized water (60.69 U/mg) proved to be the most effective in digesting protein in vitro. Malaysian Society of Animal Production 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118225/1/118225.pdf Akit, Henny and Loh, Teck Chwen and Ab Aziz, Muhamad Faris and Foo, Hooi Ling and Zailani, N. S. (2024) Pineapple peel bromelain extract improves poultry in vitro protein digestibility. Malaysian Journal of Animal Science, 27 (2). pp. 53-64. ISSN 1394-3227; eISSN: 2550-2123 https://mjas.my/mjas-v2/rf/pages/viewpaper.php?id=364 |
| spellingShingle | Akit, Henny Loh, Teck Chwen Ab Aziz, Muhamad Faris Foo, Hooi Ling Zailani, N. S. Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title | Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title_full | Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title_fullStr | Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title_full_unstemmed | Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title_short | Pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| title_sort | pineapple peel bromelain extract improves poultry in vitro protein digestibility |
| url | http://psasir.upm.edu.my/id/eprint/118225/ http://psasir.upm.edu.my/id/eprint/118225/ http://psasir.upm.edu.my/id/eprint/118225/1/118225.pdf |