Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML)
Introduction: Non-APML, CD34 and HLA-DR negative (HLA-DRneg non-APML) differ from acute promyelocytic leukaemia (APML). Morphology and blast immunophenotype alone cannot differentiate between the two; molecular confirmation is necessary (1). Nucleophosmin 1 (NPM1) is mainly found in the earlier type...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118168/ http://psasir.upm.edu.my/id/eprint/118168/1/118168.pdf |
| _version_ | 1848867449448955904 |
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| author | Seman, Zainina Mohd Tohit, Eusni Rahayu Muthu Krishnan, Amy Amutha Amanda Ho, Caroline Siew Ling |
| author_facet | Seman, Zainina Mohd Tohit, Eusni Rahayu Muthu Krishnan, Amy Amutha Amanda Ho, Caroline Siew Ling |
| author_sort | Seman, Zainina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Non-APML, CD34 and HLA-DR negative (HLA-DRneg non-APML) differ from acute promyelocytic leukaemia (APML). Morphology and blast immunophenotype alone cannot differentiate between the two; molecular confirmation is necessary (1). Nucleophosmin 1 (NPM1) is mainly found in the earlier type, while the PML-RARA mutation is characteristic of APML. This study aimed to characterise HLA-DRneg non-APML and APML through im-munophenotyping, molecular findings and post-induction chemotherapy remission rate. Materials and methods: A cross-sectional study was conducted at HSAJB, involving all cases diagnosed as acute myeloid leukaemia (AML) for 3 years. The proportions of APML and HLA-DRneg non-APML and their immunophenotypes, molecular mutations and prognosis were compared. Results: A total of 182 AML cases were analysed. There was no statistically signifi-cant difference in immunophenotypes between the two types of AML. However, more APML cases were negative for CD11c and CD14 compared to HLA-DRneg non-APML cases. Only 69 samples had positive molecular mutations. Four cases of HLA-DRneg non-APML had NPM1 mutations; another four cases had NPM1 with FLT3 ITD mutations. HLA-DRneg non-APML cases with NPM1 mutations had a relatively higher post-induction haematological remission rate than HLA-DRneg non-APML NPM1 with FLT3 ITD mutations. Conclusion: A more extensive antibody panel in flow cytometry and a larger sample size may yield better results in distinguishing the two types of AML. |
| first_indexed | 2025-11-15T14:36:40Z |
| format | Article |
| id | upm-118168 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:36:40Z |
| publishDate | 2024 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1181682025-06-26T03:47:58Z http://psasir.upm.edu.my/id/eprint/118168/ Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) Seman, Zainina Mohd Tohit, Eusni Rahayu Muthu Krishnan, Amy Amutha Amanda Ho, Caroline Siew Ling Introduction: Non-APML, CD34 and HLA-DR negative (HLA-DRneg non-APML) differ from acute promyelocytic leukaemia (APML). Morphology and blast immunophenotype alone cannot differentiate between the two; molecular confirmation is necessary (1). Nucleophosmin 1 (NPM1) is mainly found in the earlier type, while the PML-RARA mutation is characteristic of APML. This study aimed to characterise HLA-DRneg non-APML and APML through im-munophenotyping, molecular findings and post-induction chemotherapy remission rate. Materials and methods: A cross-sectional study was conducted at HSAJB, involving all cases diagnosed as acute myeloid leukaemia (AML) for 3 years. The proportions of APML and HLA-DRneg non-APML and their immunophenotypes, molecular mutations and prognosis were compared. Results: A total of 182 AML cases were analysed. There was no statistically signifi-cant difference in immunophenotypes between the two types of AML. However, more APML cases were negative for CD11c and CD14 compared to HLA-DRneg non-APML cases. Only 69 samples had positive molecular mutations. Four cases of HLA-DRneg non-APML had NPM1 mutations; another four cases had NPM1 with FLT3 ITD mutations. HLA-DRneg non-APML cases with NPM1 mutations had a relatively higher post-induction haematological remission rate than HLA-DRneg non-APML NPM1 with FLT3 ITD mutations. Conclusion: A more extensive antibody panel in flow cytometry and a larger sample size may yield better results in distinguishing the two types of AML. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/118168/1/118168.pdf Seman, Zainina and Mohd Tohit, Eusni Rahayu and Muthu Krishnan, Amy Amutha Amanda and Ho, Caroline Siew Ling (2024) Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML). Malaysian Journal of Medicine and Health Sciences, 20 (suppl.11). pp. 10-15. ISSN 1675-8544; eISSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/202412301815243_MJMHS_0460.pdf |
| spellingShingle | Seman, Zainina Mohd Tohit, Eusni Rahayu Muthu Krishnan, Amy Amutha Amanda Ho, Caroline Siew Ling Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title | Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title_full | Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title_fullStr | Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title_full_unstemmed | Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title_short | Molecular and immunophenotyping characteristics of non-APML, CD34 and HLA-DR negative acute myeloid leukaemia (HLA-DRneg non-APML) |
| title_sort | molecular and immunophenotyping characteristics of non-apml, cd34 and hla-dr negative acute myeloid leukaemia (hla-drneg non-apml) |
| url | http://psasir.upm.edu.my/id/eprint/118168/ http://psasir.upm.edu.my/id/eprint/118168/ http://psasir.upm.edu.my/id/eprint/118168/1/118168.pdf |