Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues
African swine fever was first reported in February 2021 and shares similar pathological signs with classical swine fever, which is endemic in Malaysia. This study aimed to detect the viral antigen in formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemistry and to characteris...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Veterinary Medical Association
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/118153/ http://psasir.upm.edu.my/id/eprint/118153/1/118153.pdf |
| _version_ | 1848867445662547968 |
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| author | Uda Zahli, Nurul Izzati Che' Amat, Azlan Fong, Michelle Wai Cheng Ooi, Peck Toung Phan, Y.W. |
| author_facet | Uda Zahli, Nurul Izzati Che' Amat, Azlan Fong, Michelle Wai Cheng Ooi, Peck Toung Phan, Y.W. |
| author_sort | Uda Zahli, Nurul Izzati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | African swine fever was first reported in February 2021 and shares similar pathological signs with classical swine fever, which is endemic in Malaysia. This study aimed to detect the viral antigen in formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemistry and to characterise the histopathological lesions. The FFPE tissues of spleen, tonsil and lymph nodes of 17 domestic pigs and three wild boars were collected from 2020 to 2022 from the histopathology laboratory archive. Immunohistochemistry revealed ASFV antigen-positive cells in 1/20 (5%) samples and CSFV antigen-positive cells in 4/20 (20%) samples. All CSF-positive samples show lymphoid depletion in the tonsil, lymph node and spleen tissues and 3/4 (75%) show haemorrhages in lymph node and spleen tissues. This study illustrates the need to strengthen biosecurity to prevent transmission of ASF and CSF into the farm. |
| first_indexed | 2025-11-15T14:36:37Z |
| format | Article |
| id | upm-118153 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:36:37Z |
| publishDate | 2024 |
| publisher | Malaysian Veterinary Medical Association |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1181532025-07-10T03:19:53Z http://psasir.upm.edu.my/id/eprint/118153/ Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues Uda Zahli, Nurul Izzati Che' Amat, Azlan Fong, Michelle Wai Cheng Ooi, Peck Toung Phan, Y.W. African swine fever was first reported in February 2021 and shares similar pathological signs with classical swine fever, which is endemic in Malaysia. This study aimed to detect the viral antigen in formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemistry and to characterise the histopathological lesions. The FFPE tissues of spleen, tonsil and lymph nodes of 17 domestic pigs and three wild boars were collected from 2020 to 2022 from the histopathology laboratory archive. Immunohistochemistry revealed ASFV antigen-positive cells in 1/20 (5%) samples and CSFV antigen-positive cells in 4/20 (20%) samples. All CSF-positive samples show lymphoid depletion in the tonsil, lymph node and spleen tissues and 3/4 (75%) show haemorrhages in lymph node and spleen tissues. This study illustrates the need to strengthen biosecurity to prevent transmission of ASF and CSF into the farm. Malaysian Veterinary Medical Association 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118153/1/118153.pdf Uda Zahli, Nurul Izzati and Che' Amat, Azlan and Fong, Michelle Wai Cheng and Ooi, Peck Toung and Phan, Y.W. (2024) Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues. Jurnal Veterinar Malaysia, 36 (1). pp. 25-29. ISSN 9128-2506 https://www.mavma.org.my/jvm/2024-volume-36-issue-no-1-dec |
| spellingShingle | Uda Zahli, Nurul Izzati Che' Amat, Azlan Fong, Michelle Wai Cheng Ooi, Peck Toung Phan, Y.W. Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title | Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title_full | Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title_fullStr | Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title_full_unstemmed | Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title_short | Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| title_sort | immunohistochemical detection of african swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues |
| url | http://psasir.upm.edu.my/id/eprint/118153/ http://psasir.upm.edu.my/id/eprint/118153/ http://psasir.upm.edu.my/id/eprint/118153/1/118153.pdf |