Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans

Many studies have been concerned with nanocellulose's potential to produce environmentally friendly nanomaterial fibers. Bacterial cellulose has shown superiority over plant cellulose, leading to increased research focus on bacterial cellulose production. Among bacterial species, Acetobacter, p...

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Main Authors: Muhd Noor, Noor Dina, Oslan, Siti Nurbaya, Mohd Razin, Siti Noorfathiah
Format: Article
Language:English
Published: Universiti Putra Malaysia, Universiti Pertanian Malaysia Press 2025
Online Access:http://psasir.upm.edu.my/id/eprint/118095/
http://psasir.upm.edu.my/id/eprint/118095/1/118095.crdownload
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author Muhd Noor, Noor Dina
Oslan, Siti Nurbaya
Mohd Razin, Siti Noorfathiah
author_facet Muhd Noor, Noor Dina
Oslan, Siti Nurbaya
Mohd Razin, Siti Noorfathiah
author_sort Muhd Noor, Noor Dina
building UPM Institutional Repository
collection Online Access
description Many studies have been concerned with nanocellulose's potential to produce environmentally friendly nanomaterial fibers. Bacterial cellulose has shown superiority over plant cellulose, leading to increased research focus on bacterial cellulose production. Among bacterial species, Acetobacter, particularly Komagataeibacter (formerly Gluconacetobacter), has captured interest due to its enhanced bacterial cellulose (BC) production and strain stability. Optimizing production processes becomes imperative with the growing demand for BC in various industries. This study explores the optimization of physical conditions for BC production using Komagataeibacter sucrofermentans. Five parameters—pH, temperature, aeration rate, shaking rate, and surface area, were examined using the One-factor-at-a-time (OFAT) method. This method was selected as it is useful in early-stage optimization to understand the effect of individual factors on BC production. The extracted BC was purified with 4.0 M NaOH solution at 80°C, and wet and dry weights were measured. Analysis via ANOVA determined the significance of each parameter in enhancing BC yield. Optimized conditions from this experiment —pH 5, temperature 20°C, 60% aeration rate, slow agitation (50 rpm), and large surface area fermentation (63.62 cm2) shown to give better BC production. These findings have substantial implications for enhancing BC production efficiency on an industrial scale.
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spelling upm-1180952025-06-24T08:00:08Z http://psasir.upm.edu.my/id/eprint/118095/ Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans Muhd Noor, Noor Dina Oslan, Siti Nurbaya Mohd Razin, Siti Noorfathiah Many studies have been concerned with nanocellulose's potential to produce environmentally friendly nanomaterial fibers. Bacterial cellulose has shown superiority over plant cellulose, leading to increased research focus on bacterial cellulose production. Among bacterial species, Acetobacter, particularly Komagataeibacter (formerly Gluconacetobacter), has captured interest due to its enhanced bacterial cellulose (BC) production and strain stability. Optimizing production processes becomes imperative with the growing demand for BC in various industries. This study explores the optimization of physical conditions for BC production using Komagataeibacter sucrofermentans. Five parameters—pH, temperature, aeration rate, shaking rate, and surface area, were examined using the One-factor-at-a-time (OFAT) method. This method was selected as it is useful in early-stage optimization to understand the effect of individual factors on BC production. The extracted BC was purified with 4.0 M NaOH solution at 80°C, and wet and dry weights were measured. Analysis via ANOVA determined the significance of each parameter in enhancing BC yield. Optimized conditions from this experiment —pH 5, temperature 20°C, 60% aeration rate, slow agitation (50 rpm), and large surface area fermentation (63.62 cm2) shown to give better BC production. These findings have substantial implications for enhancing BC production efficiency on an industrial scale. Universiti Putra Malaysia, Universiti Pertanian Malaysia Press 2025-03-07 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/118095/1/118095.crdownload Muhd Noor, Noor Dina and Oslan, Siti Nurbaya and Mohd Razin, Siti Noorfathiah (2025) Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans. Pertanika Journal of Science and Technology, 33 (2). pp. 893-912. ISSN 0128-7680; eISSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-5281-2024 10.47836/pjst.33.2.16
spellingShingle Muhd Noor, Noor Dina
Oslan, Siti Nurbaya
Mohd Razin, Siti Noorfathiah
Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title_full Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title_fullStr Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title_full_unstemmed Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title_short Physical parameters optimization of bacterial cellulose from Komagataeibacter sucrofermentans
title_sort physical parameters optimization of bacterial cellulose from komagataeibacter sucrofermentans
url http://psasir.upm.edu.my/id/eprint/118095/
http://psasir.upm.edu.my/id/eprint/118095/
http://psasir.upm.edu.my/id/eprint/118095/
http://psasir.upm.edu.my/id/eprint/118095/1/118095.crdownload