Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations

The white root rot (WRR) disease poses a formidable economic challenge to rubber plantations globally, with Malaysia particularly hard-hit. This disease is attributed to Rigidoporus microporus. This glasshouse experiment investigated the effects of a stored, peat moss-based formulation containing si...

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Main Authors: Shabbir, Imran, Wong, Mui-Yun, Sulaiman, Zulkefly, Shahbaz, Muhammad, Khan, Iqra, Rizwana, Humaira, Elshikh, Mohamed Soliman, Adil, Muhammad Faheem
Format: Article
Language:English
Published: Springer Science and Business Media LLC 2024
Online Access:http://psasir.upm.edu.my/id/eprint/118001/
http://psasir.upm.edu.my/id/eprint/118001/1/118001.pdf
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author Shabbir, Imran
Wong, Mui-Yun
Sulaiman, Zulkefly
Shahbaz, Muhammad
Khan, Iqra
Rizwana, Humaira
Elshikh, Mohamed Soliman
Adil, Muhammad Faheem
author_facet Shabbir, Imran
Wong, Mui-Yun
Sulaiman, Zulkefly
Shahbaz, Muhammad
Khan, Iqra
Rizwana, Humaira
Elshikh, Mohamed Soliman
Adil, Muhammad Faheem
author_sort Shabbir, Imran
building UPM Institutional Repository
collection Online Access
description The white root rot (WRR) disease poses a formidable economic challenge to rubber plantations globally, with Malaysia particularly hard-hit. This disease is attributed to Rigidoporus microporus. This glasshouse experiment investigated the effects of a stored, peat moss-based formulation containing silicon (Si), Glomus mosseae, and Enterobacter sp. UPMSSB7 on combatting WRR and promoting the growth of rubber plants. Compared to the positive control, the experimental bioformulation significantly reduced disease incidence (P < 0.0001), with efficacy comparable to the propiconazole fungicide. Furthermore, the bioformulation and fungicide treatments demonstrated superior disease mitigation compared to the positive control 24 weeks after R. microporus-inoculation. The bioformulation treatment not only reduced disease incidence and mitigated foliar and root rot symptoms, but it also resulted in a lower disease progressive curve and reduced R. microporus colonisation. Additionally, bioformulation significantly increased (P < 0.001) plant growth parameters 24 weeks after R. microporus inoculation. These parameters included stem height, girth size, chlorophyll content, leaf area, root and shoot dry weight, root volume, total root length, and root surface area. These effects surpassed those observed in fungicide and control treatments. The Si content in shoot and root and leaf N, P, and K nutrient contents were also significantly (P < 0.001) increased in the R. microporus-inoculated plants with the tested bioformulation than the fungicide and control. In the case of R. microporus-inoculated plants of bioformulation treatment, there was a significant (P < 0.001) increase in the population density of Enterobacter sp. (1.5 × 108 cfu g− 1 soil), surpassing the levels observed in non-inoculated plants of bioformulation and inoculants with Si, with or without R. microporus-inoculation. Moreover, bioformulation treatments improved (P < 0.001) root colonisation as well as spore density of G. mosseae after R. microporus-inoculation than control and fungicide. This study suggests that a peat-based bioformulation containing G. mosseae, Enterobacter sp., and Si could be an effective strategy for both enhancing plant growth and mitigating WRR in rubber plants.
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spelling upm-1180012025-06-20T02:51:49Z http://psasir.upm.edu.my/id/eprint/118001/ Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations Shabbir, Imran Wong, Mui-Yun Sulaiman, Zulkefly Shahbaz, Muhammad Khan, Iqra Rizwana, Humaira Elshikh, Mohamed Soliman Adil, Muhammad Faheem The white root rot (WRR) disease poses a formidable economic challenge to rubber plantations globally, with Malaysia particularly hard-hit. This disease is attributed to Rigidoporus microporus. This glasshouse experiment investigated the effects of a stored, peat moss-based formulation containing silicon (Si), Glomus mosseae, and Enterobacter sp. UPMSSB7 on combatting WRR and promoting the growth of rubber plants. Compared to the positive control, the experimental bioformulation significantly reduced disease incidence (P < 0.0001), with efficacy comparable to the propiconazole fungicide. Furthermore, the bioformulation and fungicide treatments demonstrated superior disease mitigation compared to the positive control 24 weeks after R. microporus-inoculation. The bioformulation treatment not only reduced disease incidence and mitigated foliar and root rot symptoms, but it also resulted in a lower disease progressive curve and reduced R. microporus colonisation. Additionally, bioformulation significantly increased (P < 0.001) plant growth parameters 24 weeks after R. microporus inoculation. These parameters included stem height, girth size, chlorophyll content, leaf area, root and shoot dry weight, root volume, total root length, and root surface area. These effects surpassed those observed in fungicide and control treatments. The Si content in shoot and root and leaf N, P, and K nutrient contents were also significantly (P < 0.001) increased in the R. microporus-inoculated plants with the tested bioformulation than the fungicide and control. In the case of R. microporus-inoculated plants of bioformulation treatment, there was a significant (P < 0.001) increase in the population density of Enterobacter sp. (1.5 × 108 cfu g− 1 soil), surpassing the levels observed in non-inoculated plants of bioformulation and inoculants with Si, with or without R. microporus-inoculation. Moreover, bioformulation treatments improved (P < 0.001) root colonisation as well as spore density of G. mosseae after R. microporus-inoculation than control and fungicide. This study suggests that a peat-based bioformulation containing G. mosseae, Enterobacter sp., and Si could be an effective strategy for both enhancing plant growth and mitigating WRR in rubber plants. Springer Science and Business Media LLC 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/118001/1/118001.pdf Shabbir, Imran and Wong, Mui-Yun and Sulaiman, Zulkefly and Shahbaz, Muhammad and Khan, Iqra and Rizwana, Humaira and Elshikh, Mohamed Soliman and Adil, Muhammad Faheem (2024) Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations. Journal of Rubber Research, 27 (5). pp. 691-702. ISSN 1511-1768; eISSN: 2524-3993 https://link.springer.com/article/10.1007/s42464-024-00289-9?error=cookies_not_supported&code=06a394bd-4053-4832-b195-59cddfc2b7b5 10.1007/s42464-024-00289-9
spellingShingle Shabbir, Imran
Wong, Mui-Yun
Sulaiman, Zulkefly
Shahbaz, Muhammad
Khan, Iqra
Rizwana, Humaira
Elshikh, Mohamed Soliman
Adil, Muhammad Faheem
Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title_full Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title_fullStr Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title_full_unstemmed Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title_short Beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
title_sort beyond fungicides: embracing bioformulation innovation in mitigating white root rot disease impact on rubber plantations
url http://psasir.upm.edu.my/id/eprint/118001/
http://psasir.upm.edu.my/id/eprint/118001/
http://psasir.upm.edu.my/id/eprint/118001/
http://psasir.upm.edu.my/id/eprint/118001/1/118001.pdf