Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review
In vitro cell culture models are crucial tools in drug discovery for understanding pathophysiology and cellular physiology. Traditionally, two-dimensional (2D) cultures have significantly advanced cell-based research. However, their translational potential is limited by their inability to replicate...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sabah
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117718/ http://psasir.upm.edu.my/id/eprint/117718/1/117718.pdf |
| _version_ | 1848867322426556416 |
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| author | Mohd Esa, Norhaizan Kamarudin, Ammar Akram Zulhilmi Cheng, Mohamad Zulhafiz Shafiq |
| author_facet | Mohd Esa, Norhaizan Kamarudin, Ammar Akram Zulhilmi Cheng, Mohamad Zulhafiz Shafiq |
| author_sort | Mohd Esa, Norhaizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In vitro cell culture models are crucial tools in drug discovery for understanding pathophysiology and cellular physiology. Traditionally, two-dimensional (2D) cultures have significantly advanced cell-based research. However, their translational potential is limited by their inability to replicate the natural cellular environment. The emergence of three-dimensional (3D) culture models offers more physiological relevance platforms to mimic a natural tissue architecture. Scaffold-based techniques that utilize extracellular matrix (ECM) components have become increasingly prominent, with collagen emerging as a leading candidate due to its structural and biological properties. Collagen, the key player of structural protein in connective tissue, may act as biomimetic scaffolds that facilitate cell aggregation, proliferation, and migration. This mini-review explores the unique features of collagen as a hydrogel scaffold, including its biocompatibility, natural abundance, and the capacity to replicate tissue-specific architectures. |
| first_indexed | 2025-11-15T14:34:39Z |
| format | Article |
| id | upm-117718 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:34:39Z |
| publishDate | 2024 |
| publisher | Universiti Malaysia Sabah |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1177182025-06-11T02:05:47Z http://psasir.upm.edu.my/id/eprint/117718/ Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review Mohd Esa, Norhaizan Kamarudin, Ammar Akram Zulhilmi Cheng, Mohamad Zulhafiz Shafiq In vitro cell culture models are crucial tools in drug discovery for understanding pathophysiology and cellular physiology. Traditionally, two-dimensional (2D) cultures have significantly advanced cell-based research. However, their translational potential is limited by their inability to replicate the natural cellular environment. The emergence of three-dimensional (3D) culture models offers more physiological relevance platforms to mimic a natural tissue architecture. Scaffold-based techniques that utilize extracellular matrix (ECM) components have become increasingly prominent, with collagen emerging as a leading candidate due to its structural and biological properties. Collagen, the key player of structural protein in connective tissue, may act as biomimetic scaffolds that facilitate cell aggregation, proliferation, and migration. This mini-review explores the unique features of collagen as a hydrogel scaffold, including its biocompatibility, natural abundance, and the capacity to replicate tissue-specific architectures. Universiti Malaysia Sabah 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117718/1/117718.pdf Mohd Esa, Norhaizan and Kamarudin, Ammar Akram and Zulhilmi Cheng, Mohamad Zulhafiz Shafiq (2024) Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review. Transactions on Science and Technology, 11 (4-2). pp. 1-10. ISSN 2289-8786 https://tost.unise.org/pdfs/vol11/no4-2/vol11n4-2.html |
| spellingShingle | Mohd Esa, Norhaizan Kamarudin, Ammar Akram Zulhilmi Cheng, Mohamad Zulhafiz Shafiq Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title | Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title_full | Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title_fullStr | Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title_full_unstemmed | Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title_short | Collagen as extracellular matrix (ECM) for three-dimensional (3D) cell culture models: A mini-review |
| title_sort | collagen as extracellular matrix (ecm) for three-dimensional (3d) cell culture models: a mini-review |
| url | http://psasir.upm.edu.my/id/eprint/117718/ http://psasir.upm.edu.my/id/eprint/117718/ http://psasir.upm.edu.my/id/eprint/117718/1/117718.pdf |