In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae
In aquaculture, using probiotics is crucial for strengthening the immune system and encouraging the growth and survival of many aquatic organisms, including the Pangasius species. This approach is particularly significant given the impact of bacterial diseases on Pangasius survival. This study aimed...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/117704/ http://psasir.upm.edu.my/id/eprint/117704/1/117704.pdf |
| _version_ | 1848867318439870464 |
|---|---|
| author | Johar, Shafiq Puvanasundram, Puvaneswari de Cruz, Clement Roy Chong, Chou Min Md Yasin, Ina-Salwany Lim, Keng Chin Mat Noordin, Noordiyana Karim, Murni |
| author_facet | Johar, Shafiq Puvanasundram, Puvaneswari de Cruz, Clement Roy Chong, Chou Min Md Yasin, Ina-Salwany Lim, Keng Chin Mat Noordin, Noordiyana Karim, Murni |
| author_sort | Johar, Shafiq |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In aquaculture, using probiotics is crucial for strengthening the immune system and encouraging the growth and survival of many aquatic organisms, including the Pangasius species. This approach is particularly significant given the impact of bacterial diseases on Pangasius survival. This study aimed to assess the effectiveness of probiotics isolated from Pangasius nasutus as alternatives to antibiotics for combating infections caused by Aeromonas hydrophila and Streptococcus agalactiae. Potential bacteria were isolated from the intestine and stomach of healthy P. nasutus. Seventy probiotic strains were successfully isolated and further screened using A. hydrophila and S. agalactiae as pathogens in an in vitro disc diffusion assay. Preliminary screenings indicated that five probiotic strains inhibited the growth of A. hydrophila. Stomach-derived strain S1 and intestine-derived strain L1 suppressed A. hydrophila growth with inhibition zones of 10.5±1 mm and 8.5±1 mm, respectively. Likewise, strains L2, L8, and L12 from the intestine showed inhibitory zones of 6.0±1 mm, 6.5±1 mm, and 6.0±1 mm, respectively. Of these, only L12 inhibited the growth of S. agalactiae with a clear zone of 5.0±1 mm. In the elimination of pathogenic strains, potential strains S1 and L1 did not grow on the Aeromonas isolation medium. Co-culture assays demonstrated that both potential strains significantly inhibited Aeromonas hydrophila growth at concentrations of 106 and 108 CFU mL-1 over 48- and 96-hour periods, respectively. The potential bacterial strains were identified using 16s rRNA gene sequencing and were classified as follows: S1 - Lactococcus lactis, L1 - Weissella confusa, L2 - Cosenzaea myxofaciens, L8 - Lactococcus garvieae, and L12 - Plesiomonas shigelloides. Strain S1 L. lactis and strain L1 W. confusa are suggested for further evaluation and acquired additional research to fully elucidate their mechanisms and potential as probiotics. |
| first_indexed | 2025-11-15T14:34:35Z |
| format | Article |
| id | upm-117704 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:34:35Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1177042025-07-10T01:25:01Z http://psasir.upm.edu.my/id/eprint/117704/ In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae Johar, Shafiq Puvanasundram, Puvaneswari de Cruz, Clement Roy Chong, Chou Min Md Yasin, Ina-Salwany Lim, Keng Chin Mat Noordin, Noordiyana Karim, Murni In aquaculture, using probiotics is crucial for strengthening the immune system and encouraging the growth and survival of many aquatic organisms, including the Pangasius species. This approach is particularly significant given the impact of bacterial diseases on Pangasius survival. This study aimed to assess the effectiveness of probiotics isolated from Pangasius nasutus as alternatives to antibiotics for combating infections caused by Aeromonas hydrophila and Streptococcus agalactiae. Potential bacteria were isolated from the intestine and stomach of healthy P. nasutus. Seventy probiotic strains were successfully isolated and further screened using A. hydrophila and S. agalactiae as pathogens in an in vitro disc diffusion assay. Preliminary screenings indicated that five probiotic strains inhibited the growth of A. hydrophila. Stomach-derived strain S1 and intestine-derived strain L1 suppressed A. hydrophila growth with inhibition zones of 10.5±1 mm and 8.5±1 mm, respectively. Likewise, strains L2, L8, and L12 from the intestine showed inhibitory zones of 6.0±1 mm, 6.5±1 mm, and 6.0±1 mm, respectively. Of these, only L12 inhibited the growth of S. agalactiae with a clear zone of 5.0±1 mm. In the elimination of pathogenic strains, potential strains S1 and L1 did not grow on the Aeromonas isolation medium. Co-culture assays demonstrated that both potential strains significantly inhibited Aeromonas hydrophila growth at concentrations of 106 and 108 CFU mL-1 over 48- and 96-hour periods, respectively. The potential bacterial strains were identified using 16s rRNA gene sequencing and were classified as follows: S1 - Lactococcus lactis, L1 - Weissella confusa, L2 - Cosenzaea myxofaciens, L8 - Lactococcus garvieae, and L12 - Plesiomonas shigelloides. Strain S1 L. lactis and strain L1 W. confusa are suggested for further evaluation and acquired additional research to fully elucidate their mechanisms and potential as probiotics. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117704/1/117704.pdf Johar, Shafiq and Puvanasundram, Puvaneswari and de Cruz, Clement Roy and Chong, Chou Min and Md Yasin, Ina-Salwany and Lim, Keng Chin and Mat Noordin, Noordiyana and Karim, Murni (2024) In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae. Sains Malaysiana, 53 (1). pp. 99-110. ISSN 0126-6039; eISSN: 0126-6039 https://www.ukm.my/jsm/pdf_files/SM-PDF-53-1-2024/8.pdf 10.17576/jsm-2024-5301-08 |
| spellingShingle | Johar, Shafiq Puvanasundram, Puvaneswari de Cruz, Clement Roy Chong, Chou Min Md Yasin, Ina-Salwany Lim, Keng Chin Mat Noordin, Noordiyana Karim, Murni In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title | In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title_full | In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title_fullStr | In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title_full_unstemmed | In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title_short | In vitro inhibitory activities of potential probiotic isolated from Pangasius nasutus against Aeromonas hydrophila and Streptococcus agalactiae |
| title_sort | in vitro inhibitory activities of potential probiotic isolated from pangasius nasutus against aeromonas hydrophila and streptococcus agalactiae |
| url | http://psasir.upm.edu.my/id/eprint/117704/ http://psasir.upm.edu.my/id/eprint/117704/ http://psasir.upm.edu.my/id/eprint/117704/ http://psasir.upm.edu.my/id/eprint/117704/1/117704.pdf |