Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction
Vitex trifolia var. purpurea leaves (Lemuni leaves) is considered as a traditional medicine due to their antioxidant property. The antioxidant properties of Lemuni leaves depends on the concentration of ethanol-water solvent. Therefore, this current study intended to identify the antioxidant activit...
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| Format: | Article |
| Language: | English |
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Talenta Publisher
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117582/ http://psasir.upm.edu.my/id/eprint/117582/1/117582.pdf |
| _version_ | 1848867288563843072 |
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| author | Goh, Wei Wen Sultana, Sharmin Azlan, Azrina |
| author_facet | Goh, Wei Wen Sultana, Sharmin Azlan, Azrina |
| author_sort | Goh, Wei Wen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vitex trifolia var. purpurea leaves (Lemuni leaves) is considered as a traditional medicine due to their antioxidant property. The antioxidant properties of Lemuni leaves depends on the concentration of ethanol-water solvent. Therefore, this current study intended to identify the antioxidant activity of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol-water solvent extraction. Four ethanol-water concentrations were prepared (water extract (0% ethanol), 25% ethanol extract, 50% ethanol extract, and 75% ethanol extract). The antioxidant properties of Lemuni leaves were evaluated by FolinCiocalteu assay, aluminium chloride calorimetric method while 2,2’-diphenyl-1picrylhydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were used to measure the antioxidant activities of the extracts. The experimental results showed that 75% ethanol extract had the highest (p<0.05) total phenolic content (94.69 ± 4.75mg GAE/g DW) and total flavonoid content (30.76 ± 2.85mg QE/g DW) significantly. The 75% ethanol extract also had the significantly lowest (p<0.05) IC50 value for DPPH (3.56 ± 0.11 mg/mL) and ABTS (2.01 ± 0.05 mg/mL) assays. Strong correlations were discovered between the total flavonoid content, total phenolic content with antioxidant properties of Lemuni leaves at different ethanol-water ratio by DPPH (r = -0.816; r = -0.824) and ABTS assays (r = -0.929; r = -0.920). The significantly elevated antioxidant properties of the 75% ethanol extract suggest promising applications in health supplements, pharmaceuticals, skincare products, biomedical research, and commercial ventures. |
| first_indexed | 2025-11-15T14:34:07Z |
| format | Article |
| id | upm-117582 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:34:07Z |
| publishDate | 2024 |
| publisher | Talenta Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1175822025-05-30T02:21:23Z http://psasir.upm.edu.my/id/eprint/117582/ Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction Goh, Wei Wen Sultana, Sharmin Azlan, Azrina Vitex trifolia var. purpurea leaves (Lemuni leaves) is considered as a traditional medicine due to their antioxidant property. The antioxidant properties of Lemuni leaves depends on the concentration of ethanol-water solvent. Therefore, this current study intended to identify the antioxidant activity of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol-water solvent extraction. Four ethanol-water concentrations were prepared (water extract (0% ethanol), 25% ethanol extract, 50% ethanol extract, and 75% ethanol extract). The antioxidant properties of Lemuni leaves were evaluated by FolinCiocalteu assay, aluminium chloride calorimetric method while 2,2’-diphenyl-1picrylhydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were used to measure the antioxidant activities of the extracts. The experimental results showed that 75% ethanol extract had the highest (p<0.05) total phenolic content (94.69 ± 4.75mg GAE/g DW) and total flavonoid content (30.76 ± 2.85mg QE/g DW) significantly. The 75% ethanol extract also had the significantly lowest (p<0.05) IC50 value for DPPH (3.56 ± 0.11 mg/mL) and ABTS (2.01 ± 0.05 mg/mL) assays. Strong correlations were discovered between the total flavonoid content, total phenolic content with antioxidant properties of Lemuni leaves at different ethanol-water ratio by DPPH (r = -0.816; r = -0.824) and ABTS assays (r = -0.929; r = -0.920). The significantly elevated antioxidant properties of the 75% ethanol extract suggest promising applications in health supplements, pharmaceuticals, skincare products, biomedical research, and commercial ventures. Talenta Publisher 2024 Article PeerReviewed text en cc_by_nc_sa_4 http://psasir.upm.edu.my/id/eprint/117582/1/117582.pdf Goh, Wei Wen and Sultana, Sharmin and Azlan, Azrina (2024) Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction. Indonesian Journal of Agricultural Research, 7 (2). pp. 106-118. ISSN 2622-7681; eISSN: 2615-5842 https://talenta.usu.ac.id/InJAR/article/view/13357 10.32734/injar.v7i2.13357 |
| spellingShingle | Goh, Wei Wen Sultana, Sharmin Azlan, Azrina Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title | Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title_full | Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title_fullStr | Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title_full_unstemmed | Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title_short | Antioxidant properties of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| title_sort | antioxidant properties of lemuni leaves (vitex trifolia var. purpurea) in different concentrations of ethanol- water solvent extraction |
| url | http://psasir.upm.edu.my/id/eprint/117582/ http://psasir.upm.edu.my/id/eprint/117582/ http://psasir.upm.edu.my/id/eprint/117582/ http://psasir.upm.edu.my/id/eprint/117582/1/117582.pdf |