Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights

2-(3’,4’-dihydroxyphenyl)-3,6,7,8-tetramethoxy-4H-chromen-4-one (3), a new flavonoid was isolated from the ethyl acetate fraction of Chrysanthemum morifolium Ramat. dried flowers, while 4′,5-dihydroxy-3,3′,7,8-tetramethoxy-flavone (1) and casticine (5) were isolated from this plant for the first tim...

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Main Authors: Tey, Zi Tong, Loh, Khye Er, Tan, Siow-Ping, Yuan, Chongjun, Tejo, Bimo Ario, Ismail, Intan Safinar, Nafiah, Mohd Azlan
Format: Article
Language:English
Published: Elsevier 2024
Online Access:http://psasir.upm.edu.my/id/eprint/117569/
http://psasir.upm.edu.my/id/eprint/117569/1/117569.pdf
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author Tey, Zi Tong
Loh, Khye Er
Tan, Siow-Ping
Yuan, Chongjun
Tejo, Bimo Ario
Ismail, Intan Safinar
Nafiah, Mohd Azlan
author_facet Tey, Zi Tong
Loh, Khye Er
Tan, Siow-Ping
Yuan, Chongjun
Tejo, Bimo Ario
Ismail, Intan Safinar
Nafiah, Mohd Azlan
author_sort Tey, Zi Tong
building UPM Institutional Repository
collection Online Access
description 2-(3’,4’-dihydroxyphenyl)-3,6,7,8-tetramethoxy-4H-chromen-4-one (3), a new flavonoid was isolated from the ethyl acetate fraction of Chrysanthemum morifolium Ramat. dried flowers, while 4′,5-dihydroxy-3,3′,7,8-tetramethoxy-flavone (1) and casticine (5) were isolated from this plant for the first time, as well as two known flavonoids, eriodictyol-7-O-glucoside (2) and luteolin (4). Their structures were investigated over a broad spatial scale using chromatographic and spectroscopic techniques (NMR, FTIR, UV and HR-ESI-MS). Compound 4 showed the highest XO inhibition and the most favorable interaction with XO in molecular docking analysis. Compound 2, 3 and 4 demonstrated stable interactions with catalytically important residues in xanthine oxidase via molecular dynamic analysis.
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institution Universiti Putra Malaysia
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publisher Elsevier
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spelling upm-1175692025-07-09T08:43:24Z http://psasir.upm.edu.my/id/eprint/117569/ Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights Tey, Zi Tong Loh, Khye Er Tan, Siow-Ping Yuan, Chongjun Tejo, Bimo Ario Ismail, Intan Safinar Nafiah, Mohd Azlan 2-(3’,4’-dihydroxyphenyl)-3,6,7,8-tetramethoxy-4H-chromen-4-one (3), a new flavonoid was isolated from the ethyl acetate fraction of Chrysanthemum morifolium Ramat. dried flowers, while 4′,5-dihydroxy-3,3′,7,8-tetramethoxy-flavone (1) and casticine (5) were isolated from this plant for the first time, as well as two known flavonoids, eriodictyol-7-O-glucoside (2) and luteolin (4). Their structures were investigated over a broad spatial scale using chromatographic and spectroscopic techniques (NMR, FTIR, UV and HR-ESI-MS). Compound 4 showed the highest XO inhibition and the most favorable interaction with XO in molecular docking analysis. Compound 2, 3 and 4 demonstrated stable interactions with catalytically important residues in xanthine oxidase via molecular dynamic analysis. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117569/1/117569.pdf Tey, Zi Tong and Loh, Khye Er and Tan, Siow-Ping and Yuan, Chongjun and Tejo, Bimo Ario and Ismail, Intan Safinar and Nafiah, Mohd Azlan (2024) Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights. Phytochemistry Letters, 64. pp. 68-78. ISSN 1874-3900 https://linkinghub.elsevier.com/retrieve/pii/S1874390024001393 10.1016/j.phytol.2024.10.002
spellingShingle Tey, Zi Tong
Loh, Khye Er
Tan, Siow-Ping
Yuan, Chongjun
Tejo, Bimo Ario
Ismail, Intan Safinar
Nafiah, Mohd Azlan
Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title_full Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title_fullStr Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title_full_unstemmed Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title_short Xanthine oxidase inhibitory activity by flavonoids from Chrysanthemum morifolium: in vitro and in silico insights
title_sort xanthine oxidase inhibitory activity by flavonoids from chrysanthemum morifolium: in vitro and in silico insights
url http://psasir.upm.edu.my/id/eprint/117569/
http://psasir.upm.edu.my/id/eprint/117569/
http://psasir.upm.edu.my/id/eprint/117569/
http://psasir.upm.edu.my/id/eprint/117569/1/117569.pdf