Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes)
Water pollution due to plasticizers is one of the most severe environmental problems worldwide. Phthalate plasticizers can act as endocrine disruptors in vertebrates. In this study, we investigated whether the non-phthalate bis(2-ethylhexyl) sebacate (DEHS) plasticizer can act as an endocrine disrup...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117352/ http://psasir.upm.edu.my/id/eprint/117352/1/117352.pdf |
| _version_ | 1848867226039353344 |
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| author | Horie, Yoshifumi Nomura, Miho Ramaswamy, Babu Rajendran Harino, Hiroya Yap, Chee Kong Okamura, Hideo |
| author_facet | Horie, Yoshifumi Nomura, Miho Ramaswamy, Babu Rajendran Harino, Hiroya Yap, Chee Kong Okamura, Hideo |
| author_sort | Horie, Yoshifumi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Water pollution due to plasticizers is one of the most severe environmental problems worldwide. Phthalate plasticizers can act as endocrine disruptors in vertebrates. In this study, we investigated whether the non-phthalate bis(2-ethylhexyl) sebacate (DEHS) plasticizer can act as an endocrine disruptor by evaluating changes in the expression levels of thyroid hormone-related, reproduction-related, and estrogen-responsive genes of Japanese medaka (Oryzias latipes) exposed to the plasticizer. Following the exposure, the gene expression levels of thyroid-stimulating hormone subunit beta (tshβ), deiodinase 1 (dio1), and thyroid hormone receptor alpha (trα) did not change. Meanwhile, DEHS suppressed dio2 expression, did not induce swim bladder inflation, and eventually reduced the swimming performance of Japanese medaka. These findings indicate that DEHS can potentially disrupt the thyroid hormone-related gene expression and metabolism of these fish. However, exposure to DEHS did not induce changes in the gene expression levels of kisspeptin 1 (kiss1), gonadotropin-releasing hormone (gnrh), follicle-stimulating hormone beta (fshβ), luteinizing hormone beta (lhβ), choriogenin H (chgH), and vitellogenin (vtg) in a dose-dependent manner. This is the first report providing evidence that DEHS can disrupt thyroid hormone-related metabolism in fish. |
| first_indexed | 2025-11-15T14:33:07Z |
| format | Article |
| id | upm-117352 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:33:07Z |
| publishDate | 2023 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1173522025-07-08T01:57:09Z http://psasir.upm.edu.my/id/eprint/117352/ Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) Horie, Yoshifumi Nomura, Miho Ramaswamy, Babu Rajendran Harino, Hiroya Yap, Chee Kong Okamura, Hideo Water pollution due to plasticizers is one of the most severe environmental problems worldwide. Phthalate plasticizers can act as endocrine disruptors in vertebrates. In this study, we investigated whether the non-phthalate bis(2-ethylhexyl) sebacate (DEHS) plasticizer can act as an endocrine disruptor by evaluating changes in the expression levels of thyroid hormone-related, reproduction-related, and estrogen-responsive genes of Japanese medaka (Oryzias latipes) exposed to the plasticizer. Following the exposure, the gene expression levels of thyroid-stimulating hormone subunit beta (tshβ), deiodinase 1 (dio1), and thyroid hormone receptor alpha (trα) did not change. Meanwhile, DEHS suppressed dio2 expression, did not induce swim bladder inflation, and eventually reduced the swimming performance of Japanese medaka. These findings indicate that DEHS can potentially disrupt the thyroid hormone-related gene expression and metabolism of these fish. However, exposure to DEHS did not induce changes in the gene expression levels of kisspeptin 1 (kiss1), gonadotropin-releasing hormone (gnrh), follicle-stimulating hormone beta (fshβ), luteinizing hormone beta (lhβ), choriogenin H (chgH), and vitellogenin (vtg) in a dose-dependent manner. This is the first report providing evidence that DEHS can disrupt thyroid hormone-related metabolism in fish. Elsevier 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117352/1/117352.pdf Horie, Yoshifumi and Nomura, Miho and Ramaswamy, Babu Rajendran and Harino, Hiroya and Yap, Chee Kong and Okamura, Hideo (2023) Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes). Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 264. art. no. 109531. pp. 1-9. ISSN 1532-0456; eISSN: 1878-1659 https://linkinghub.elsevier.com/retrieve/pii/S1532045622002666 10.1016/j.cbpc.2022.109531 |
| spellingShingle | Horie, Yoshifumi Nomura, Miho Ramaswamy, Babu Rajendran Harino, Hiroya Yap, Chee Kong Okamura, Hideo Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title | Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title_full | Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title_fullStr | Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title_full_unstemmed | Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title_short | Effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (DEHS) on the endocrine system in Japanese medaka (Oryzias latipes) |
| title_sort | effects of non-phthalate plasticizer bis(2-ethylhexyl) sebacate (dehs) on the endocrine system in japanese medaka (oryzias latipes) |
| url | http://psasir.upm.edu.my/id/eprint/117352/ http://psasir.upm.edu.my/id/eprint/117352/ http://psasir.upm.edu.my/id/eprint/117352/ http://psasir.upm.edu.my/id/eprint/117352/1/117352.pdf |