Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats
Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance, inte...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia (UKM Press)
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117227/ http://psasir.upm.edu.my/id/eprint/117227/1/117227.pdf |
| _version_ | 1848867194139574272 |
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| author | Shaharudin, Muhammad Firdhaus Shafie, Siti Raihanah Abdullah, Maizaton Atmadini Abd Ghani, Fauzah Sabran, Mohd Redzwan |
| author_facet | Shaharudin, Muhammad Firdhaus Shafie, Siti Raihanah Abdullah, Maizaton Atmadini Abd Ghani, Fauzah Sabran, Mohd Redzwan |
| author_sort | Shaharudin, Muhammad Firdhaus |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance, intestinal health, and excretion of faecal AFB1 metabolite of AFB1-exposed rats. Thirty two male Sprague Dawley rats were divided into control, AFB1, AFB1+LcS and LcS groups. AFB1 was given at a complete dosage of 25 µg AFB1/kg body weight, while LcS supplementation at 2×10^9 CFU/mL per day for four weeks. The average body weight of the AFB1 group showed no significant increase from week 2 to 4, while other groups had an increment throughout the study. The food intake of the AFB1 and AFB1+LcS groups had significantly reduced throughout the treatment period. AFB1 exposure caused several changes in the histomorphometry parameters but was normalised with LcS supplementation. The AFB1 group showed mild to moderate inflammation in all intestinal parts, whereas only mild inflammation was observed in the jejunum and ileum of the AFB1+LcS group. Faecal Bifidobacterium spp. counts showed an increment in three groups, while the AFB1 group showed a significant reduction. The faecal AFB1 in the AFB1 group was significantly lower than in the AFB1+LcS group. In conclusion, AFB1 affected growth performance and intestinal health, and wherein the effects were alleviated by LcS supplementation. Further investigation on intestinal permeability and serum and urinary AFB1 level is suggested to understand the mechanism of probiotic-AFB1 interaction in alleviating AFB1 toxicity. |
| first_indexed | 2025-11-15T14:32:37Z |
| format | Article |
| id | upm-117227 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:32:37Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia (UKM Press) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1172272025-05-02T08:07:41Z http://psasir.upm.edu.my/id/eprint/117227/ Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats Shaharudin, Muhammad Firdhaus Shafie, Siti Raihanah Abdullah, Maizaton Atmadini Abd Ghani, Fauzah Sabran, Mohd Redzwan Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance, intestinal health, and excretion of faecal AFB1 metabolite of AFB1-exposed rats. Thirty two male Sprague Dawley rats were divided into control, AFB1, AFB1+LcS and LcS groups. AFB1 was given at a complete dosage of 25 µg AFB1/kg body weight, while LcS supplementation at 2×10^9 CFU/mL per day for four weeks. The average body weight of the AFB1 group showed no significant increase from week 2 to 4, while other groups had an increment throughout the study. The food intake of the AFB1 and AFB1+LcS groups had significantly reduced throughout the treatment period. AFB1 exposure caused several changes in the histomorphometry parameters but was normalised with LcS supplementation. The AFB1 group showed mild to moderate inflammation in all intestinal parts, whereas only mild inflammation was observed in the jejunum and ileum of the AFB1+LcS group. Faecal Bifidobacterium spp. counts showed an increment in three groups, while the AFB1 group showed a significant reduction. The faecal AFB1 in the AFB1 group was significantly lower than in the AFB1+LcS group. In conclusion, AFB1 affected growth performance and intestinal health, and wherein the effects were alleviated by LcS supplementation. Further investigation on intestinal permeability and serum and urinary AFB1 level is suggested to understand the mechanism of probiotic-AFB1 interaction in alleviating AFB1 toxicity. Penerbit Universiti Kebangsaan Malaysia (UKM Press) 2024 Article PeerReviewed text en cc_by_nc_sa_4 http://psasir.upm.edu.my/id/eprint/117227/1/117227.pdf Shaharudin, Muhammad Firdhaus and Shafie, Siti Raihanah and Abdullah, Maizaton Atmadini and Abd Ghani, Fauzah and Sabran, Mohd Redzwan (2024) Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats. Jurnal Sains Kesihatan Malaysia, 22 (2). pp. 67-82. ISSN 1675-8161; eISSN: 2289-4535 https://ejournal.ukm.my/jskm/article/view/65470 10.17576/jskm-2024-2202-05 |
| spellingShingle | Shaharudin, Muhammad Firdhaus Shafie, Siti Raihanah Abdullah, Maizaton Atmadini Abd Ghani, Fauzah Sabran, Mohd Redzwan Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title | Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title_full | Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title_fullStr | Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title_full_unstemmed | Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title_short | Effects of Lacticaseibacillus paracasei Shirota supplementation on growth performance, intestinal health, and fecal AFB1 metabolite in AFB1-exposed rats |
| title_sort | effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal afb1 metabolite in afb1-exposed rats |
| url | http://psasir.upm.edu.my/id/eprint/117227/ http://psasir.upm.edu.my/id/eprint/117227/ http://psasir.upm.edu.my/id/eprint/117227/ http://psasir.upm.edu.my/id/eprint/117227/1/117227.pdf |