Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping
Current uric acid detection methodologies lack the requisite sensitivity and selectivity for point-of-care applications. Plasmonic sensors, while promising, demand refinement for improved performance. This work introduces a biofunctionalized sensor predicated on surface plasmon resonance to quantify...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117150/ http://psasir.upm.edu.my/id/eprint/117150/1/117150.pdf |
| _version_ | 1848867176348385280 |
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| author | Onifade, Olabisi Abdullahi Yusairie, Fatin Adriena Abu Bakar, Muhammad Hafiz Alresheedi, Mohammed Thamer Khoon Ng, Eng Mahdi, Mohd Adzir Muhammad Noor, Ahmad Shukri |
| author_facet | Onifade, Olabisi Abdullahi Yusairie, Fatin Adriena Abu Bakar, Muhammad Hafiz Alresheedi, Mohammed Thamer Khoon Ng, Eng Mahdi, Mohd Adzir Muhammad Noor, Ahmad Shukri |
| author_sort | Onifade, Olabisi Abdullahi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Current uric acid detection methodologies lack the requisite sensitivity and selectivity for point-of-care applications. Plasmonic sensors, while promising, demand refinement for improved performance. This work introduces a biofunctionalized sensor predicated on surface plasmon resonance to quantify uric acid within physiologically relevant concentration ranges. The sensor employs the covalent immobilization of uricase enzyme using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) crosslinking agents, ensuring the durable adherence of the enzyme onto the sensor probe. Characterization through atomic force microscopy and Fourier transform infrared spectroscopy validate surface alterations. The Langmuir adsorption isotherm model elucidates binding kinetics, revealing a sensor binding affinity of 298.83 (mg/dL)−1, and a maximum adsorption capacity of approximately 1.0751°. The biofunctionalized sensor exhibits a sensitivity of 0.0755°/(mg/dL), a linear correlation coefficient of 0.8313, and a limit of detection of 0.095 mg/dL. Selectivity tests against potentially competing interferents like glucose, ascorbic acid, urea, D-cystine, and creatinine showcase a significant resonance angle shift of 1.1135° for uric acid compared to 0.1853° for interferents at the same concentration. Significantly, at a low uric acid concentration of 0.5 mg/dL, a distinct shift of 0.3706° was observed, setting it apart from the lower values noticed at higher concentrations for all typical interferent samples. The uricase enzyme significantly enhances plasmonic sensors for uric acid detection, showcasing a seamless integration of optical principles and biological recognition elements. These sensors hold promise as vital tools in clinical and point-of-care settings, offering transformative potential in biosensing technologies and the potential to revolutionize healthcare outcomes in biomedicine. |
| first_indexed | 2025-11-15T14:32:20Z |
| format | Article |
| id | upm-117150 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:32:20Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1171502025-07-07T09:34:29Z http://psasir.upm.edu.my/id/eprint/117150/ Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping Onifade, Olabisi Abdullahi Yusairie, Fatin Adriena Abu Bakar, Muhammad Hafiz Alresheedi, Mohammed Thamer Khoon Ng, Eng Mahdi, Mohd Adzir Muhammad Noor, Ahmad Shukri Current uric acid detection methodologies lack the requisite sensitivity and selectivity for point-of-care applications. Plasmonic sensors, while promising, demand refinement for improved performance. This work introduces a biofunctionalized sensor predicated on surface plasmon resonance to quantify uric acid within physiologically relevant concentration ranges. The sensor employs the covalent immobilization of uricase enzyme using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) crosslinking agents, ensuring the durable adherence of the enzyme onto the sensor probe. Characterization through atomic force microscopy and Fourier transform infrared spectroscopy validate surface alterations. The Langmuir adsorption isotherm model elucidates binding kinetics, revealing a sensor binding affinity of 298.83 (mg/dL)−1, and a maximum adsorption capacity of approximately 1.0751°. The biofunctionalized sensor exhibits a sensitivity of 0.0755°/(mg/dL), a linear correlation coefficient of 0.8313, and a limit of detection of 0.095 mg/dL. Selectivity tests against potentially competing interferents like glucose, ascorbic acid, urea, D-cystine, and creatinine showcase a significant resonance angle shift of 1.1135° for uric acid compared to 0.1853° for interferents at the same concentration. Significantly, at a low uric acid concentration of 0.5 mg/dL, a distinct shift of 0.3706° was observed, setting it apart from the lower values noticed at higher concentrations for all typical interferent samples. The uricase enzyme significantly enhances plasmonic sensors for uric acid detection, showcasing a seamless integration of optical principles and biological recognition elements. These sensors hold promise as vital tools in clinical and point-of-care settings, offering transformative potential in biosensing technologies and the potential to revolutionize healthcare outcomes in biomedicine. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/117150/1/117150.pdf Onifade, Olabisi Abdullahi and Yusairie, Fatin Adriena and Abu Bakar, Muhammad Hafiz and Alresheedi, Mohammed Thamer and Khoon Ng, Eng and Mahdi, Mohd Adzir and Muhammad Noor, Ahmad Shukri (2024) Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping. Biosensors and Bioelectronics, 261. pp. 1-11. ISSN 0956-5663; eISSN: 1873-4235 https://www.sciencedirect.com/science/article/pii/S0956566324004913 10.1016/j.bios.2024.116486 |
| spellingShingle | Onifade, Olabisi Abdullahi Yusairie, Fatin Adriena Abu Bakar, Muhammad Hafiz Alresheedi, Mohammed Thamer Khoon Ng, Eng Mahdi, Mohd Adzir Muhammad Noor, Ahmad Shukri Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title | Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title_full | Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title_fullStr | Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title_full_unstemmed | Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title_short | Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping |
| title_sort | uricase biofunctionalized plasmonic sensor for uric acid detection with aptes-modified gold nanotopping |
| url | http://psasir.upm.edu.my/id/eprint/117150/ http://psasir.upm.edu.my/id/eprint/117150/ http://psasir.upm.edu.my/id/eprint/117150/ http://psasir.upm.edu.my/id/eprint/117150/1/117150.pdf |