Effects of green silver nanoparticles on bacterial-infected in vitro Eucalyptus pellita F.Muell plant
Nanotechnology has emerged as a vibrant field of research in several modern scientific and engineering fields. The application and development of silver nanoparticles (AgNPs) have remarkably increased due to their distinctive features, including size (1-100 nm), shape, and antimicrobial propertie...
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| Format: | Thesis |
| Language: | English |
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2023
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| Online Access: | http://psasir.upm.edu.my/id/eprint/117033/ http://psasir.upm.edu.my/id/eprint/117033/1/117033.pdf |
| Summary: | Nanotechnology has emerged as a vibrant field of research in several modern scientific
and engineering fields. The application and development of silver nanoparticles (AgNPs)
have remarkably increased due to their distinctive features, including size (1-100 nm),
shape, and antimicrobial properties. Diverse biomolecules and biological entities
(prokaryotes and eukaryotes) are widely used as bio-stocks to create nanostructured
materials. Green AgNPs provide non-toxic and sustainable materials to be used as
antimicrobial agents. The growing demand for Eucalyptus species for industrial
applications requires an efficient propagation strategy for rapidly and genetically
producing improved trees. However, various microbial pathogens affecting the growth
of Eucalyptus, which are considered the main concerns since they can cause long-term
damage to Eucalyptus plantation. This study examined the effect of green AgNPs on the
pathogen-infected in vitro Eucalyptus pellita (E. pellita) plants. Shoot regenerated in
vitro from cotyledonary leaf explants of E. pellita were induced through direct
organogenesis using (Murashige and Skoog) MS media supplemented with different
concentrations (0.4, 1.3, 2.2, 3.1, 3.9 μM) of thidiazuron (TDZ), 6-benzylaminopurine
(BAP) and kinetin (KIN). The elongated shoots were rooted in vitro using ½ MS media
supplemented with different concentrations (0.5, 1, 1.5, 2, 2.5 μM) of indole-3-butyric
acid (IBA) and 1-naphthaleneacetic acid (1-NAA), respectively.The highest shoot
formation from cotyledonary leaves was observed in the MS media supplemented with
2.2 μM BAP, followed by 3.1 μM BAP, giving a mean of 7.4 and 6 shoots per explant,
respectively. The highest root formation was observed in the ½ MS media supplemented
with 1.5 μM IBA averaging 17.47 roots per explant. The regenerated plantlets were
infected with three bacterial strains (Bacillus sp. EU_UPM1, Pntoea dispersa
EU_UPM2 and Pntoea dispersa EU_UPM3). The results showed that the antibacterial
effect of green AgNPs on infected plants was evaluated in vitro by the direct inoculation
of 100 ppm of green AgNPs to the bacterial infected E. pellita plantlets. This research
will create awareness of new aspirations to mass-produce the free pathogen-infected E.
pellita plants. |
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