Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans
Galactosylkojic acid, in which the hydroxymethyl group of the kojic acid moiety is β-galactosylated, was synthesized by using a transgalactosidation reaction with an immobilized β-galactosidase from Bacillus circulans. With 5% kojic acid and 20% lactose as the substrates, the yield with respect to t...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group LLC
1995
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116991/ http://psasir.upm.edu.my/id/eprint/116991/1/116991.pdf |
| _version_ | 1848867138015592448 |
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| author | Ali Hassan, Mohd. Ismail, Fadhilah Yamamoto, Shuichi Yamada, Hidenori Nakanishi, Kazuhiro |
| author_facet | Ali Hassan, Mohd. Ismail, Fadhilah Yamamoto, Shuichi Yamada, Hidenori Nakanishi, Kazuhiro |
| author_sort | Ali Hassan, Mohd. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Galactosylkojic acid, in which the hydroxymethyl group of the kojic acid moiety is β-galactosylated, was synthesized by using a transgalactosidation reaction with an immobilized β-galactosidase from Bacillus circulans. With 5% kojic acid and 20% lactose as the substrates, the yield with respect to the initial amount of kojic acid was around 24% as a maximum. The inhibitory effect of galactosylkojic acid towards the oxidation reaction of L-β-(3,4-dihydroxyphenyl)alanine (L-DOPA) with tyrosinase was similar to that of kojic acid. |
| first_indexed | 2025-11-15T14:31:43Z |
| format | Article |
| id | upm-116991 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:31:43Z |
| publishDate | 1995 |
| publisher | Taylor & Francis Group LLC |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1169912025-04-22T06:36:37Z http://psasir.upm.edu.my/id/eprint/116991/ Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans Ali Hassan, Mohd. Ismail, Fadhilah Yamamoto, Shuichi Yamada, Hidenori Nakanishi, Kazuhiro Galactosylkojic acid, in which the hydroxymethyl group of the kojic acid moiety is β-galactosylated, was synthesized by using a transgalactosidation reaction with an immobilized β-galactosidase from Bacillus circulans. With 5% kojic acid and 20% lactose as the substrates, the yield with respect to the initial amount of kojic acid was around 24% as a maximum. The inhibitory effect of galactosylkojic acid towards the oxidation reaction of L-β-(3,4-dihydroxyphenyl)alanine (L-DOPA) with tyrosinase was similar to that of kojic acid. Taylor & Francis Group LLC 1995 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116991/1/116991.pdf Ali Hassan, Mohd. and Ismail, Fadhilah and Yamamoto, Shuichi and Yamada, Hidenori and Nakanishi, Kazuhiro (1995) Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans. Bioscience, Biotechnology and Biochemistry, 59 (3). pp. 543-545. ISSN 0916-8451; eISSN: 1347-6947 https://academic.oup.com/bbb/article/59/3/543-545/5951325 10.1271/bbb.59.543 |
| spellingShingle | Ali Hassan, Mohd. Ismail, Fadhilah Yamamoto, Shuichi Yamada, Hidenori Nakanishi, Kazuhiro Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title | Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title_full | Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title_fullStr | Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title_full_unstemmed | Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title_short | Enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| title_sort | enzymatic synthesis of galactosylkojic acid with immobilized β-galactosidase from bacillus circulans |
| url | http://psasir.upm.edu.my/id/eprint/116991/ http://psasir.upm.edu.my/id/eprint/116991/ http://psasir.upm.edu.my/id/eprint/116991/ http://psasir.upm.edu.my/id/eprint/116991/1/116991.pdf |