Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer
Dipterocarpus, commonly known as 'keruing,' is an important source of dammarane and contributes to a highly valuable economic plant in Southeast Asia. A preliminary study revealed that this plant is rich in phenolic compounds and potential antimicrobial properties. Thus, the stem bark of t...
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| Format: | Article |
| Language: | English |
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Malaysian Institute of Chemistry
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116956/ http://psasir.upm.edu.my/id/eprint/116956/1/116956.pdf |
| _version_ | 1848867130146029568 |
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| author | Mohd Zain, Wan Zuraida Wan Yusoff, Nor Asma Husna Rukayadi, Yaya Aziman, Nurain Windayani, Neneng |
| author_facet | Mohd Zain, Wan Zuraida Wan Yusoff, Nor Asma Husna Rukayadi, Yaya Aziman, Nurain Windayani, Neneng |
| author_sort | Mohd Zain, Wan Zuraida Wan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dipterocarpus, commonly known as 'keruing,' is an important source of dammarane and contributes to a highly valuable economic plant in Southeast Asia. A preliminary study revealed that this plant is rich in phenolic compounds and potential antimicrobial properties. Thus, the stem bark of three Dipterocarpus species (Dipterocarpus verrucosus, Dipterocarpus cornutus, and Dipterocarpus crinitus) has been extensively studied chemically and biologically. The methanol extract was isolated using multiple chromatography techniques, and the structural elucidation of the compounds was characterized using UV, IR, NMR (1d &2D), HRESI-MS, and comparison with literature. The anticandidal activity of the methanolic crude extracts and compounds was determined using the disc diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and time-kill assay against pathogenic strains, namely Candida glabrata. In this study, three crude extracts and 12 phytochemicals of consisting of nine stilbenoids (viniferin, ampelopsin A, α-viniferin, davidiol A, stenophyllol B, ampelopsin E, vaticanol B, diptoindonesin E, Hemsleyanol D, two phenolic compounds (bergenin, scopoletin) and one compound from terpene (β-sitosterol glucoside) were tested for their anticandidal activity. The disc diffusion method result showed that ε-viniferin was more susceptible to C. glabrata than other compounds; thus, this compound was selected for time-kill assay. The MIC and MFC ranged from 62.5 to 500 ppm. Time-kill curves demonstrated that ε-viniferin could inhibit C. glabrata strains at 500 ppm, after 120 min of treatment with a significant reduction of more than 3 log10 reduction. Results revealed the potential of viniferin isolated from Dipterocarpus to be developed as an anticandidal agent against C. glabrata. |
| first_indexed | 2025-11-15T14:31:36Z |
| format | Article |
| id | upm-116956 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:31:36Z |
| publishDate | 2024 |
| publisher | Malaysian Institute of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1169562025-04-21T01:08:27Z http://psasir.upm.edu.my/id/eprint/116956/ Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer Mohd Zain, Wan Zuraida Wan Yusoff, Nor Asma Husna Rukayadi, Yaya Aziman, Nurain Windayani, Neneng Dipterocarpus, commonly known as 'keruing,' is an important source of dammarane and contributes to a highly valuable economic plant in Southeast Asia. A preliminary study revealed that this plant is rich in phenolic compounds and potential antimicrobial properties. Thus, the stem bark of three Dipterocarpus species (Dipterocarpus verrucosus, Dipterocarpus cornutus, and Dipterocarpus crinitus) has been extensively studied chemically and biologically. The methanol extract was isolated using multiple chromatography techniques, and the structural elucidation of the compounds was characterized using UV, IR, NMR (1d &2D), HRESI-MS, and comparison with literature. The anticandidal activity of the methanolic crude extracts and compounds was determined using the disc diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and time-kill assay against pathogenic strains, namely Candida glabrata. In this study, three crude extracts and 12 phytochemicals of consisting of nine stilbenoids (viniferin, ampelopsin A, α-viniferin, davidiol A, stenophyllol B, ampelopsin E, vaticanol B, diptoindonesin E, Hemsleyanol D, two phenolic compounds (bergenin, scopoletin) and one compound from terpene (β-sitosterol glucoside) were tested for their anticandidal activity. The disc diffusion method result showed that ε-viniferin was more susceptible to C. glabrata than other compounds; thus, this compound was selected for time-kill assay. The MIC and MFC ranged from 62.5 to 500 ppm. Time-kill curves demonstrated that ε-viniferin could inhibit C. glabrata strains at 500 ppm, after 120 min of treatment with a significant reduction of more than 3 log10 reduction. Results revealed the potential of viniferin isolated from Dipterocarpus to be developed as an anticandidal agent against C. glabrata. Malaysian Institute of Chemistry 2024-02-26 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116956/1/116956.pdf Mohd Zain, Wan Zuraida Wan and Yusoff, Nor Asma Husna and Rukayadi, Yaya and Aziman, Nurain and Windayani, Neneng (2024) Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer. Malaysian Journal of Chemistry, 26 (1). pp. 302-313. ISSN 1511-2292; eISSN: 2550-1658 https://ikm.org.my/publications/malaysian-journal-of-chemistry/view-abstract.php?abs=J0048-3b52d1f 10.55373/mjchem.v26i1.302 |
| spellingShingle | Mohd Zain, Wan Zuraida Wan Yusoff, Nor Asma Husna Rukayadi, Yaya Aziman, Nurain Windayani, Neneng Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title | Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title_full | Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title_fullStr | Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title_full_unstemmed | Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title_short | Anti-candidal activity of crude extracts and compounds from Dipterocarpus verrucosus Foxw. Ex Sloot, Dipterocarpus cornutus Dyer and Dipterocarpus crinitus Dyer |
| title_sort | anti-candidal activity of crude extracts and compounds from dipterocarpus verrucosus foxw. ex sloot, dipterocarpus cornutus dyer and dipterocarpus crinitus dyer |
| url | http://psasir.upm.edu.my/id/eprint/116956/ http://psasir.upm.edu.my/id/eprint/116956/ http://psasir.upm.edu.my/id/eprint/116956/ http://psasir.upm.edu.my/id/eprint/116956/1/116956.pdf |