The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line

Background: Ardisia crenata (A. crenata) and Peliosanthes teta Andrew (P. teta) are popular medicinal plants in Malaysia that are claimed to have several biological effects. Objective: To assess the anticancer and radical-scavenging properties of A. crenata and P. teta methanolic extracts. Methods:...

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Main Authors: Al-Zubaidi, Yassir Mohammed Ali, Abdul Kadir, Arifah, Zakaria, Zainul Amiruddin
Format: Article
Language:English
Published: Al-Rafidain University College 2024
Online Access:http://psasir.upm.edu.my/id/eprint/116935/
http://psasir.upm.edu.my/id/eprint/116935/1/116935.pdf
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author Al-Zubaidi, Yassir Mohammed Ali
Abdul Kadir, Arifah
Zakaria, Zainul Amiruddin
author_facet Al-Zubaidi, Yassir Mohammed Ali
Abdul Kadir, Arifah
Zakaria, Zainul Amiruddin
author_sort Al-Zubaidi, Yassir Mohammed Ali
building UPM Institutional Repository
collection Online Access
description Background: Ardisia crenata (A. crenata) and Peliosanthes teta Andrew (P. teta) are popular medicinal plants in Malaysia that are claimed to have several biological effects. Objective: To assess the anticancer and radical-scavenging properties of A. crenata and P. teta methanolic extracts. Methods: TPC was quantified using the Folin-Ciocalteu test; free radical scavenging was evaluated using the 2,2'-azino-bis3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays; and cytotoxic activity was assessed using the MTT assay. Results: In both ABTS and DPPH systems, A. crenata exhibits much higher radical scavenging activity than ascorbic acid. P. teta showed less significant antioxidant activity. Furthermore, A. crenata methanolic extracts demonstrated free radical scavenging properties in a concentration-dependent manner, quenching ABTS and DPPH radicals with no lag phase observed in the experiments. Furthermore, A. crenata had a strong cytotoxic effect on the breast cancer 4T1 cell line but had no suppression of cell viability in the 3T3 normal fibroblast cell line. Interestingly, the TPC of A. crenata was significantly higher than that of P. teta methanolic extracts. Conclusions: A. crenata showed antioxidant and anticancer action against breast cancer 4T1 cells, indicating that it could be a valuable source for future pharmacological and medical research.
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spelling upm-1169352025-04-18T03:43:01Z http://psasir.upm.edu.my/id/eprint/116935/ The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line Al-Zubaidi, Yassir Mohammed Ali Abdul Kadir, Arifah Zakaria, Zainul Amiruddin Background: Ardisia crenata (A. crenata) and Peliosanthes teta Andrew (P. teta) are popular medicinal plants in Malaysia that are claimed to have several biological effects. Objective: To assess the anticancer and radical-scavenging properties of A. crenata and P. teta methanolic extracts. Methods: TPC was quantified using the Folin-Ciocalteu test; free radical scavenging was evaluated using the 2,2'-azino-bis3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays; and cytotoxic activity was assessed using the MTT assay. Results: In both ABTS and DPPH systems, A. crenata exhibits much higher radical scavenging activity than ascorbic acid. P. teta showed less significant antioxidant activity. Furthermore, A. crenata methanolic extracts demonstrated free radical scavenging properties in a concentration-dependent manner, quenching ABTS and DPPH radicals with no lag phase observed in the experiments. Furthermore, A. crenata had a strong cytotoxic effect on the breast cancer 4T1 cell line but had no suppression of cell viability in the 3T3 normal fibroblast cell line. Interestingly, the TPC of A. crenata was significantly higher than that of P. teta methanolic extracts. Conclusions: A. crenata showed antioxidant and anticancer action against breast cancer 4T1 cells, indicating that it could be a valuable source for future pharmacological and medical research. Al-Rafidain University College 2024-10 Article PeerReviewed text en cc_by_nc_sa_4 http://psasir.upm.edu.my/id/eprint/116935/1/116935.pdf Al-Zubaidi, Yassir Mohammed Ali and Abdul Kadir, Arifah and Zakaria, Zainul Amiruddin (2024) The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line. Al-Rafidain Journal of Medical Sciences, 7 (2). pp. 101-108. ISSN 2789-3219; eISSN: 2789-3219 https://ajms.iq/index.php/ALRAFIDAIN/article/view/1373 10.54133/ajms.v7i2.1373
spellingShingle Al-Zubaidi, Yassir Mohammed Ali
Abdul Kadir, Arifah
Zakaria, Zainul Amiruddin
The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title_full The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title_fullStr The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title_full_unstemmed The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title_short The free radical scavenging and cytotoxic properties of Ardisia crenata and Peliosanthes teta in the 4T1 breast cancer cell line
title_sort free radical scavenging and cytotoxic properties of ardisia crenata and peliosanthes teta in the 4t1 breast cancer cell line
url http://psasir.upm.edu.my/id/eprint/116935/
http://psasir.upm.edu.my/id/eprint/116935/
http://psasir.upm.edu.my/id/eprint/116935/
http://psasir.upm.edu.my/id/eprint/116935/1/116935.pdf