Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa
Spermatozoa from river and swamp water buffalo (Bubalus bubalis), goat, and pig were stained using a silver nitrate procedure and examined under bright field optics. The silver nitrate differentiated many detailed morphologic features of the head, midpiece, and tail of spermatozoa between the specie...
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| Format: | Article |
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Informa UK Limited
1983
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116930/ |
| _version_ | 1848867122428510208 |
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| author | Bongso, T.A. |
| author_facet | Bongso, T.A. |
| author_sort | Bongso, T.A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Spermatozoa from river and swamp water buffalo (Bubalus bubalis), goat, and pig were stained using a silver nitrate procedure and examined under bright field optics. The silver nitrate differentiated many detailed morphologic features of the head, midpiece, and tail of spermatozoa between the species studied. Acrosomal integrity due to sperm injury or aging and various sperm abnormalities were also clearly identified by silver nitrate. Silver staining patterns revealed species-specific and strain-specific differences, particularly of the sperm head. The biochemical basis of silver staining has been attributed to the presence of sulphydryl and disulphide-rich proteins. The technique is relatively inexpensive, rapid, and repeatable and may be useful for biological research and evaluation of semen for artificial insemination. |
| first_indexed | 2025-11-15T14:31:29Z |
| format | Article |
| id | upm-116930 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:31:29Z |
| publishDate | 1983 |
| publisher | Informa UK Limited |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1169302025-04-18T03:30:31Z http://psasir.upm.edu.my/id/eprint/116930/ Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa Bongso, T.A. Spermatozoa from river and swamp water buffalo (Bubalus bubalis), goat, and pig were stained using a silver nitrate procedure and examined under bright field optics. The silver nitrate differentiated many detailed morphologic features of the head, midpiece, and tail of spermatozoa between the species studied. Acrosomal integrity due to sperm injury or aging and various sperm abnormalities were also clearly identified by silver nitrate. Silver staining patterns revealed species-specific and strain-specific differences, particularly of the sperm head. The biochemical basis of silver staining has been attributed to the presence of sulphydryl and disulphide-rich proteins. The technique is relatively inexpensive, rapid, and repeatable and may be useful for biological research and evaluation of semen for artificial insemination. Informa UK Limited 1983-03 Article PeerReviewed Bongso, T.A. (1983) Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa. Archives of Andrology, 11 (1). pp. 13-17. ISSN 0148-5016; eISSN: 0148-5016 https://www.tandfonline.com/doi/full/10.3109/01485018308987453 10.3109/01485018308987453 |
| spellingShingle | Bongso, T.A. Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title | Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title_full | Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title_fullStr | Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title_full_unstemmed | Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title_short | Comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| title_sort | comparative silver staining patterns of water buffalo, goat, and pig spermatozoa |
| url | http://psasir.upm.edu.my/id/eprint/116930/ http://psasir.upm.edu.my/id/eprint/116930/ http://psasir.upm.edu.my/id/eprint/116930/ |