Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2
A study to determine the immunoglobulin and cellular responses in the respiratory tract of goats following intranasal exposures to formalin-killed Pasteurella haemolytica A2 was carried out. Forty-two goats were divided into two groups. Goats in Group 1 were subjected to double intranasal exposures...
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| Format: | Article |
| Language: | English |
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Elsevier Science B.V.
1999
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116752/ http://psasir.upm.edu.my/id/eprint/116752/1/116752.pdf |
| _version_ | 1848867082885660672 |
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| author | Zamri-Saad, M. Effendy, A.W.M. Israf, D.A. Azmi, M.L. |
| author_facet | Zamri-Saad, M. Effendy, A.W.M. Israf, D.A. Azmi, M.L. |
| author_sort | Zamri-Saad, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A study to determine the immunoglobulin and cellular responses in the respiratory tract of goats following intranasal exposures to formalin-killed Pasteurella haemolytica A2 was carried out. Forty-two goats were divided into two groups. Goats in Group 1 were subjected to double intranasal exposures to formalin-killed P. haemolytica A2 while goats in Group 2 were the unexposed control. Prior to and at weekly intervals post-exposure, three goats from each group were killed, serum samples were collected while the lungs were flushed with 50ml normal saline before the right apical lobes were fixed in 10% buffered formalin. Both serum and lung lavage fluid were subjected to enzyme-linked immunosorbent assay (ELISA) to determine the levels of IgA, IgM and IgG while the formalin-fixed tissues were examined histologically. IgA levels in the lung lavage fluid increased rapidly to reach a significantly (p<0.05) high level as early as Week 2 post-exposure and remained significantly (p<0.05) high throughout the study period. The IgM levels increased at an intermediate rate to reach a significantly (p<0.05) high level at Week 3 post-exposure before they decreased to an insignificant (p>0.05) level the following week and the weeks thereafter. IgG levels increased gradually and only reached a significantly (p<0.01) high level at Weeks 5 and 6 of the study. The size of the bronchus-associated lymphoid tissue (BALT) and the number of lymphocytes in BALT increased significantly from Week 2 and remained high thereafter. However, differences in the numbers of BALT were insignificant (p>0.05) initially before becoming significantly (p<0.05) high at Weeks 5 and 6. The BALT responses were parallel to those of immunoglobulins in the lung lavage fluid. |
| first_indexed | 2025-11-15T14:30:51Z |
| format | Article |
| id | upm-116752 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:30:51Z |
| publishDate | 1999 |
| publisher | Elsevier Science B.V. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1167522025-07-04T08:31:59Z http://psasir.upm.edu.my/id/eprint/116752/ Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 Zamri-Saad, M. Effendy, A.W.M. Israf, D.A. Azmi, M.L. A study to determine the immunoglobulin and cellular responses in the respiratory tract of goats following intranasal exposures to formalin-killed Pasteurella haemolytica A2 was carried out. Forty-two goats were divided into two groups. Goats in Group 1 were subjected to double intranasal exposures to formalin-killed P. haemolytica A2 while goats in Group 2 were the unexposed control. Prior to and at weekly intervals post-exposure, three goats from each group were killed, serum samples were collected while the lungs were flushed with 50ml normal saline before the right apical lobes were fixed in 10% buffered formalin. Both serum and lung lavage fluid were subjected to enzyme-linked immunosorbent assay (ELISA) to determine the levels of IgA, IgM and IgG while the formalin-fixed tissues were examined histologically. IgA levels in the lung lavage fluid increased rapidly to reach a significantly (p<0.05) high level as early as Week 2 post-exposure and remained significantly (p<0.05) high throughout the study period. The IgM levels increased at an intermediate rate to reach a significantly (p<0.05) high level at Week 3 post-exposure before they decreased to an insignificant (p>0.05) level the following week and the weeks thereafter. IgG levels increased gradually and only reached a significantly (p<0.01) high level at Weeks 5 and 6 of the study. The size of the bronchus-associated lymphoid tissue (BALT) and the number of lymphocytes in BALT increased significantly from Week 2 and remained high thereafter. However, differences in the numbers of BALT were insignificant (p>0.05) initially before becoming significantly (p<0.05) high at Weeks 5 and 6. The BALT responses were parallel to those of immunoglobulins in the lung lavage fluid. Elsevier Science B.V. 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116752/1/116752.pdf Zamri-Saad, M. and Effendy, A.W.M. and Israf, D.A. and Azmi, M.L. (1999) Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2. Veterinary Microbiology, 65 (3). pp. 233-240. ISSN 0378-1135 https://linkinghub.elsevier.com/retrieve/pii/S0378113598002983 10.1016/S0378-1135(98)00298-3 |
| spellingShingle | Zamri-Saad, M. Effendy, A.W.M. Israf, D.A. Azmi, M.L. Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title | Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title_full | Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title_fullStr | Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title_full_unstemmed | Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title_short | Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2 |
| title_sort | cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed pasteurella haemolytica a2 |
| url | http://psasir.upm.edu.my/id/eprint/116752/ http://psasir.upm.edu.my/id/eprint/116752/ http://psasir.upm.edu.my/id/eprint/116752/ http://psasir.upm.edu.my/id/eprint/116752/1/116752.pdf |