Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library
The capsular polysaccharide Vi antigen (ViCPS) is an essential virulence factor and also a protective antigen of Salmonella enterica serovar Typhi. A random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific m...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116734/ http://psasir.upm.edu.my/id/eprint/116734/1/116734.pdf |
| _version_ | 1848867077651169280 |
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| author | Tang, Swee-Seong Tan, Wen-Siang Devi, Shamala Wang, Lin-Fa Pang, Tikki Thong, Kwai-Lin |
| author_facet | Tang, Swee-Seong Tan, Wen-Siang Devi, Shamala Wang, Lin-Fa Pang, Tikki Thong, Kwai-Lin |
| author_sort | Tang, Swee-Seong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The capsular polysaccharide Vi antigen (ViCPS) is an essential virulence factor and also a protective antigen of Salmonella enterica serovar Typhi. A random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific monoclonal antibody (MAb) ATVi. Approximately 75% of the phage clones selected in the fourth round carried the peptide sequence TSHHDSHGLHRV, and the rest of the clones harbored ENHSPVNIAHKL and other related sequences. These two sequences were also obtained in a similar panning process by using pooled sera from patients with a confirmed diagnosis of typhoid fever, suggesting they mimic immunodominant epitopes of ViCPS antigens. Binding of MAb ATVi to the mimotopes was specifically blocked by ViCPS, indicating that they interact with the same binding site (paratope) of the MAb. Data and reagents generated in this study have important implications for the development of peptide-base diagnostic tests and peptide vaccines and may also provide a better understanding of the pathogenesis of typhoid fever. |
| first_indexed | 2025-11-15T14:30:46Z |
| format | Article |
| id | upm-116734 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:30:46Z |
| publishDate | 2003 |
| publisher | American Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1167342025-04-15T06:03:48Z http://psasir.upm.edu.my/id/eprint/116734/ Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library Tang, Swee-Seong Tan, Wen-Siang Devi, Shamala Wang, Lin-Fa Pang, Tikki Thong, Kwai-Lin The capsular polysaccharide Vi antigen (ViCPS) is an essential virulence factor and also a protective antigen of Salmonella enterica serovar Typhi. A random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific monoclonal antibody (MAb) ATVi. Approximately 75% of the phage clones selected in the fourth round carried the peptide sequence TSHHDSHGLHRV, and the rest of the clones harbored ENHSPVNIAHKL and other related sequences. These two sequences were also obtained in a similar panning process by using pooled sera from patients with a confirmed diagnosis of typhoid fever, suggesting they mimic immunodominant epitopes of ViCPS antigens. Binding of MAb ATVi to the mimotopes was specifically blocked by ViCPS, indicating that they interact with the same binding site (paratope) of the MAb. Data and reagents generated in this study have important implications for the development of peptide-base diagnostic tests and peptide vaccines and may also provide a better understanding of the pathogenesis of typhoid fever. American Society for Microbiology 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116734/1/116734.pdf Tang, Swee-Seong and Tan, Wen-Siang and Devi, Shamala and Wang, Lin-Fa and Pang, Tikki and Thong, Kwai-Lin (2003) Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library. Clinical and Diagnostic Laboratory Immunology, 10 (6). pp. 1078-1084. ISSN 1071-412X https://journals.asm.org/doi/10.1128/CDLI.10.6.1078-1084.2003 10.1128/CDLI.10.6.1078-1084.2003 |
| spellingShingle | Tang, Swee-Seong Tan, Wen-Siang Devi, Shamala Wang, Lin-Fa Pang, Tikki Thong, Kwai-Lin Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title | Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title_full | Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title_fullStr | Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title_full_unstemmed | Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title_short | Mimotopes of the Vi antigen of Salmonella enterica serovar Typhi identified from phage display peptide library |
| title_sort | mimotopes of the vi antigen of salmonella enterica serovar typhi identified from phage display peptide library |
| url | http://psasir.upm.edu.my/id/eprint/116734/ http://psasir.upm.edu.my/id/eprint/116734/ http://psasir.upm.edu.my/id/eprint/116734/ http://psasir.upm.edu.my/id/eprint/116734/1/116734.pdf |