Hairy root culture: A reliable bioreactor from transgenic plants
Plants can produce diverse arrays of specialized metabolites with different biological activities, which are used as raw materials in cosmetics, flavors, fragrances, pigments, and pharmaceutical industries. However, their production is quite low due to the limited availability of tissue and the comp...
| Main Authors: | , , , , , , , |
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| Format: | Book Section |
| Language: | English |
| Published: |
Elsevier
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116668/ http://psasir.upm.edu.my/id/eprint/116668/1/116668.pdf |
| _version_ | 1848867064072110080 |
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| author | Shaharuddin, Noor Azmi Atabaki, Narges Nulit, Rosimah Ahmad, Siti Aqlima Malik, Sonia Vahedi, Maryam Kalhori, Nahid Abiri, Rambod |
| author_facet | Shaharuddin, Noor Azmi Atabaki, Narges Nulit, Rosimah Ahmad, Siti Aqlima Malik, Sonia Vahedi, Maryam Kalhori, Nahid Abiri, Rambod |
| author_sort | Shaharuddin, Noor Azmi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Plants can produce diverse arrays of specialized metabolites with different biological activities, which are used as raw materials in cosmetics, flavors, fragrances, pigments, and pharmaceutical industries. However, their production is quite low due to the limited availability of tissue and the complex pathways involved. Hairy root culture (HRC) is an alternative technique to produce these compounds of interest under in vitro conditions. Hairy roots, also known as transformed roots, are a fast-growing system with genetic and biochemical stability. They have also been applied as an economical technique in phytoremediation to remove environmental pollutants. They utilize multienzyme biosynthetic pathways and display highly efficient micropropagation and genetic engineering procedures. The present review summarizes the different biotechnological tools to harness the potential of the HRC system for various applications, especially the production of specialized metabolites and phytoremediation. Studies using omics, multigene engineering, elicitation, and nanoparticles for efficient utilization of HRC systems, keeping in view the susceptible and recalcitrant plants for hairy root induction, are highlighted. Finally, bottlenecks for upscaling hairy roots to foster the production of specialized metabolites and future prospectives are outlined. |
| first_indexed | 2025-11-15T14:30:33Z |
| format | Book Section |
| id | upm-116668 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:30:33Z |
| publishDate | 2024 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1166682025-07-04T03:20:02Z http://psasir.upm.edu.my/id/eprint/116668/ Hairy root culture: A reliable bioreactor from transgenic plants Shaharuddin, Noor Azmi Atabaki, Narges Nulit, Rosimah Ahmad, Siti Aqlima Malik, Sonia Vahedi, Maryam Kalhori, Nahid Abiri, Rambod Plants can produce diverse arrays of specialized metabolites with different biological activities, which are used as raw materials in cosmetics, flavors, fragrances, pigments, and pharmaceutical industries. However, their production is quite low due to the limited availability of tissue and the complex pathways involved. Hairy root culture (HRC) is an alternative technique to produce these compounds of interest under in vitro conditions. Hairy roots, also known as transformed roots, are a fast-growing system with genetic and biochemical stability. They have also been applied as an economical technique in phytoremediation to remove environmental pollutants. They utilize multienzyme biosynthetic pathways and display highly efficient micropropagation and genetic engineering procedures. The present review summarizes the different biotechnological tools to harness the potential of the HRC system for various applications, especially the production of specialized metabolites and phytoremediation. Studies using omics, multigene engineering, elicitation, and nanoparticles for efficient utilization of HRC systems, keeping in view the susceptible and recalcitrant plants for hairy root induction, are highlighted. Finally, bottlenecks for upscaling hairy roots to foster the production of specialized metabolites and future prospectives are outlined. Elsevier 2024 Book Section PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116668/1/116668.pdf Shaharuddin, Noor Azmi and Atabaki, Narges and Nulit, Rosimah and Ahmad, Siti Aqlima and Malik, Sonia and Vahedi, Maryam and Kalhori, Nahid and Abiri, Rambod (2024) Hairy root culture: A reliable bioreactor from transgenic plants. In: Peptide and Protein Drug Delivery Using Polysaccharides. Elsevier, U.S.A, pp. 25-50. ISBN 9780443189258 https://www.sciencedirect.com/science/article/abs/pii/B9780443189258000131 10.1016/B978-0-443-18925-8.00013-1 |
| spellingShingle | Shaharuddin, Noor Azmi Atabaki, Narges Nulit, Rosimah Ahmad, Siti Aqlima Malik, Sonia Vahedi, Maryam Kalhori, Nahid Abiri, Rambod Hairy root culture: A reliable bioreactor from transgenic plants |
| title | Hairy root culture: A reliable bioreactor from transgenic plants |
| title_full | Hairy root culture: A reliable bioreactor from transgenic plants |
| title_fullStr | Hairy root culture: A reliable bioreactor from transgenic plants |
| title_full_unstemmed | Hairy root culture: A reliable bioreactor from transgenic plants |
| title_short | Hairy root culture: A reliable bioreactor from transgenic plants |
| title_sort | hairy root culture: a reliable bioreactor from transgenic plants |
| url | http://psasir.upm.edu.my/id/eprint/116668/ http://psasir.upm.edu.my/id/eprint/116668/ http://psasir.upm.edu.my/id/eprint/116668/ http://psasir.upm.edu.my/id/eprint/116668/1/116668.pdf |