Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX
Streptococcus outbreak caused mainly by Streptococcus agalactiaeleads to massive mortalities of freshwater aquatic organisms, especially cultured tilapias. This has caused a huge concern in the Malaysian aquaculture industry due to a lower aquaculture production affected by the outbreak. Thus, there...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher Enterprise
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116619/ http://psasir.upm.edu.my/id/eprint/116619/1/116619.pdf |
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| author | Gan, ZiXuen Tan, Pei Qin Halim, Murni Yasid, Nur Adeela Yusof, Mohd Termizi Hashim, Amalia Mohd Abdullah, Jaafar Rahman, Nik Mohd Afizan Nik Abd Yasin, Ina Salwany Md Hamzah, Firdaus Mohamad Shukor, Mohd Yunus Wasoh, Helmi |
| author_facet | Gan, ZiXuen Tan, Pei Qin Halim, Murni Yasid, Nur Adeela Yusof, Mohd Termizi Hashim, Amalia Mohd Abdullah, Jaafar Rahman, Nik Mohd Afizan Nik Abd Yasin, Ina Salwany Md Hamzah, Firdaus Mohamad Shukor, Mohd Yunus Wasoh, Helmi |
| author_sort | Gan, ZiXuen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Streptococcus outbreak caused mainly by Streptococcus agalactiaeleads to massive mortalities of freshwater aquatic organisms, especially cultured tilapias. This has caused a huge concern in the Malaysian aquaculture industry due to a lower aquaculture production affected by the outbreak. Thus, there is a need for a rapid and simple method to detect and monitor S. agalactiaein cultured fishponds. This study aims to investigate the characteristics of a local S. agalactiaestrain TP540K and its potential as a target bacterium to develop DNA aptamer through the whole-cell based ‘systematic evolution of ligands by exponential enrichment (SELEX)’ procedure. The morphology and biochemical characteristics of S. agalactiaestrain TP540K were studied. Furthermore, the preliminary stage of the SELEX process was carried out by incubating S. agalactiae strain TP540K with a single-stranded DNA library. In this study, S. agalactiae strain TP540K was confirmed to be spherical, gram-positive, catalase and oxidase-negative with beta-haemolytic characteristics. Additionally, the presence of bands with an estimated size of 80-90 base pairs on the agarose gel indicated the presence of DNA aptamer that could bind towards S. agalactiae strain TP540K during the SELEX process. The findings herein demonstrate that S. agalactiae strain TP540K has the potential to be utilised as a target for the production of DNA aptamers, and subsequent rounds of whole-cell based SELEX process can be performed to investigate the binding affinity and specificity for more accurate detection of S. agalactiae in a future application |
| first_indexed | 2025-11-15T14:30:20Z |
| format | Article |
| id | upm-116619 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:30:20Z |
| publishDate | 2024 |
| publisher | Hibiscus Publisher Enterprise |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1166192025-04-14T03:37:35Z http://psasir.upm.edu.my/id/eprint/116619/ Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX Gan, ZiXuen Tan, Pei Qin Halim, Murni Yasid, Nur Adeela Yusof, Mohd Termizi Hashim, Amalia Mohd Abdullah, Jaafar Rahman, Nik Mohd Afizan Nik Abd Yasin, Ina Salwany Md Hamzah, Firdaus Mohamad Shukor, Mohd Yunus Wasoh, Helmi Streptococcus outbreak caused mainly by Streptococcus agalactiaeleads to massive mortalities of freshwater aquatic organisms, especially cultured tilapias. This has caused a huge concern in the Malaysian aquaculture industry due to a lower aquaculture production affected by the outbreak. Thus, there is a need for a rapid and simple method to detect and monitor S. agalactiaein cultured fishponds. This study aims to investigate the characteristics of a local S. agalactiaestrain TP540K and its potential as a target bacterium to develop DNA aptamer through the whole-cell based ‘systematic evolution of ligands by exponential enrichment (SELEX)’ procedure. The morphology and biochemical characteristics of S. agalactiaestrain TP540K were studied. Furthermore, the preliminary stage of the SELEX process was carried out by incubating S. agalactiae strain TP540K with a single-stranded DNA library. In this study, S. agalactiae strain TP540K was confirmed to be spherical, gram-positive, catalase and oxidase-negative with beta-haemolytic characteristics. Additionally, the presence of bands with an estimated size of 80-90 base pairs on the agarose gel indicated the presence of DNA aptamer that could bind towards S. agalactiae strain TP540K during the SELEX process. The findings herein demonstrate that S. agalactiae strain TP540K has the potential to be utilised as a target for the production of DNA aptamers, and subsequent rounds of whole-cell based SELEX process can be performed to investigate the binding affinity and specificity for more accurate detection of S. agalactiae in a future application Hibiscus Publisher Enterprise 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116619/1/116619.pdf Gan, ZiXuen and Tan, Pei Qin and Halim, Murni and Yasid, Nur Adeela and Yusof, Mohd Termizi and Hashim, Amalia Mohd and Abdullah, Jaafar and Rahman, Nik Mohd Afizan Nik Abd and Yasin, Ina Salwany Md and Hamzah, Firdaus Mohamad and Shukor, Mohd Yunus and Wasoh, Helmi (2024) Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX. Journal of Environmental Microbiology and Toxicology, 12 (2). pp. 31-37. ISSN 2289-5906 https://journal.hibiscuspublisher.com/index.php/JEMAT/article/view/1010 10.54987/jemat.v12i2.1010 |
| spellingShingle | Gan, ZiXuen Tan, Pei Qin Halim, Murni Yasid, Nur Adeela Yusof, Mohd Termizi Hashim, Amalia Mohd Abdullah, Jaafar Rahman, Nik Mohd Afizan Nik Abd Yasin, Ina Salwany Md Hamzah, Firdaus Mohamad Shukor, Mohd Yunus Wasoh, Helmi Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title | Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title_full | Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title_fullStr | Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title_full_unstemmed | Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title_short | Characterization of Streptococcus agalactiaeStrain TP540K and its potential as a target for DNA aptamer development in the preliminary Stage of Whole-Cell Based SELEX |
| title_sort | characterization of streptococcus agalactiaestrain tp540k and its potential as a target for dna aptamer development in the preliminary stage of whole-cell based selex |
| url | http://psasir.upm.edu.my/id/eprint/116619/ http://psasir.upm.edu.my/id/eprint/116619/ http://psasir.upm.edu.my/id/eprint/116619/ http://psasir.upm.edu.my/id/eprint/116619/1/116619.pdf |