Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)

Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correl...

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Main Authors: Mohd Jamaludin, Nina Farhana, Engliman, Nurul Sakinah, Abdul Manaf, Shareena Fairuz, Idrus, Syazwani, Abdullah, Luqman Chuah, Jamali, Nur Syakina
Format: Article
Language:English
Published: Elsevier 2024
Online Access:http://psasir.upm.edu.my/id/eprint/116600/
http://psasir.upm.edu.my/id/eprint/116600/1/116600.pdf
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author Mohd Jamaludin, Nina Farhana
Engliman, Nurul Sakinah
Abdul Manaf, Shareena Fairuz
Idrus, Syazwani
Abdullah, Luqman Chuah
Jamali, Nur Syakina
author_facet Mohd Jamaludin, Nina Farhana
Engliman, Nurul Sakinah
Abdul Manaf, Shareena Fairuz
Idrus, Syazwani
Abdullah, Luqman Chuah
Jamali, Nur Syakina
author_sort Mohd Jamaludin, Nina Farhana
building UPM Institutional Repository
collection Online Access
description Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correlating with Gompertz constant of Hm = 974.99 mL, Rm = 17.90 mL/h and λ = 1.70 h. At 60 °C, the highest HY was obtained at 2.75 ± 0.11 mol H2/mol sugar. These corresponded to the Gompertz constant of Hm = 934.58 mL, Rm = 18.78 mL/h and λ = 1.89 h. The optimal agitation obtained was at 120 rpm, attaining the highest HY of 3.44 ± 0.54 mol H2/mol sugar, corresponding to the Gompertz constant of Hm = 1054.86 mL, Rm = 24.37 mL/h and λ = 3.90 h. The rRNA sequencing result revealed that the predominant species in the study was Thermoanaerobacterium. This study provides critical insight into process control conditions on biohydrogen production.
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institution Universiti Putra Malaysia
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spelling upm-1166002025-07-07T08:26:38Z http://psasir.upm.edu.my/id/eprint/116600/ Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4) Mohd Jamaludin, Nina Farhana Engliman, Nurul Sakinah Abdul Manaf, Shareena Fairuz Idrus, Syazwani Abdullah, Luqman Chuah Jamali, Nur Syakina Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correlating with Gompertz constant of Hm = 974.99 mL, Rm = 17.90 mL/h and λ = 1.70 h. At 60 °C, the highest HY was obtained at 2.75 ± 0.11 mol H2/mol sugar. These corresponded to the Gompertz constant of Hm = 934.58 mL, Rm = 18.78 mL/h and λ = 1.89 h. The optimal agitation obtained was at 120 rpm, attaining the highest HY of 3.44 ± 0.54 mol H2/mol sugar, corresponding to the Gompertz constant of Hm = 1054.86 mL, Rm = 24.37 mL/h and λ = 3.90 h. The rRNA sequencing result revealed that the predominant species in the study was Thermoanaerobacterium. This study provides critical insight into process control conditions on biohydrogen production. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116600/1/116600.pdf Mohd Jamaludin, Nina Farhana and Engliman, Nurul Sakinah and Abdul Manaf, Shareena Fairuz and Idrus, Syazwani and Abdullah, Luqman Chuah and Jamali, Nur Syakina (2024) Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4). Chemical Engineering Journal, 485. art. no. 149980. pp. 1-11. ISSN 1385-8947; eISSN: 1385-8947 https://linkinghub.elsevier.com/retrieve/pii/S1385894724014669 10.1016/j.cej.2024.149980
spellingShingle Mohd Jamaludin, Nina Farhana
Engliman, Nurul Sakinah
Abdul Manaf, Shareena Fairuz
Idrus, Syazwani
Abdullah, Luqman Chuah
Jamali, Nur Syakina
Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title_full Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title_fullStr Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title_full_unstemmed Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title_short Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)
title_sort effect of ph, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (gac-nife3o4)
url http://psasir.upm.edu.my/id/eprint/116600/
http://psasir.upm.edu.my/id/eprint/116600/
http://psasir.upm.edu.my/id/eprint/116600/
http://psasir.upm.edu.my/id/eprint/116600/1/116600.pdf