Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle

Aims: The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. Method and Results: By using batch fermentation, it was found that phytase production of M. jalalu...

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Main Authors: Lan, G.Q., Abdullah, N., Jalaludin, S., Ho, Y.W.
Format: Article
Language:English
Published: Blackwell Publishing 2002
Online Access:http://psasir.upm.edu.my/id/eprint/116570/
http://psasir.upm.edu.my/id/eprint/116570/1/116570.pdf
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author Lan, G.Q.
Abdullah, N.
Jalaludin, S.
Ho, Y.W.
author_facet Lan, G.Q.
Abdullah, N.
Jalaludin, S.
Ho, Y.W.
author_sort Lan, G.Q.
building UPM Institutional Repository
collection Online Access
description Aims: The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. Method and Results: By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by sodium phytate. Inclusion of glucose into rice bran-soybean milk (RB-SM) medium inhibited the phytase production. Supplementation of 0.05-0.5% of phosphate to RB-SM medium neither inhibited nor stimulated the phytase production. The optimum pH and temperature for the phytase production of M. jalaludinii were 7.02 and 39°C, respectively. Surfactants such as Tween-80 (0.5% and 1.0%) and Triton X-100 (1.0%) did not affect the phytase production. Conclusions, Significance and Impact of the Study: The study has provided important data on the culture conditions affecting phytase production of M. jalaludinii, which will facilitate future research on the optimization of fermentation processes for production of high phytase activity by M. jalaludinii.
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spelling upm-1165702025-07-03T07:22:37Z http://psasir.upm.edu.my/id/eprint/116570/ Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle Lan, G.Q. Abdullah, N. Jalaludin, S. Ho, Y.W. Aims: The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. Method and Results: By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by sodium phytate. Inclusion of glucose into rice bran-soybean milk (RB-SM) medium inhibited the phytase production. Supplementation of 0.05-0.5% of phosphate to RB-SM medium neither inhibited nor stimulated the phytase production. The optimum pH and temperature for the phytase production of M. jalaludinii were 7.02 and 39°C, respectively. Surfactants such as Tween-80 (0.5% and 1.0%) and Triton X-100 (1.0%) did not affect the phytase production. Conclusions, Significance and Impact of the Study: The study has provided important data on the culture conditions affecting phytase production of M. jalaludinii, which will facilitate future research on the optimization of fermentation processes for production of high phytase activity by M. jalaludinii. Blackwell Publishing 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116570/1/116570.pdf Lan, G.Q. and Abdullah, N. and Jalaludin, S. and Ho, Y.W. (2002) Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle. Journal of Applied Microbiology, 93 (4). pp. 668-674. ISSN 1364-5072 https://academic.oup.com/jambio/article/93/4/668/6847846 10.1046/j.1365-2672.2002.01727.x
spellingShingle Lan, G.Q.
Abdullah, N.
Jalaludin, S.
Ho, Y.W.
Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title_full Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title_fullStr Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title_full_unstemmed Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title_short Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
title_sort culture conditions influencing phytase production of mitsuokella jalaludinii, a new bacterial species from the rumen of cattle
url http://psasir.upm.edu.my/id/eprint/116570/
http://psasir.upm.edu.my/id/eprint/116570/
http://psasir.upm.edu.my/id/eprint/116570/
http://psasir.upm.edu.my/id/eprint/116570/1/116570.pdf