Anti-hyperlipidemic potential of a combination of Zingiber officinale Roscoe, Allium sativum L., Citrus lemon (L.) Osbeck, Honey and Malus domestica Borkh. Cider Vinegar
Intervention of herbal combination is widely used to decrease the risk of metabolic disorders such as cardiovascular diseases, hyperlipidemia, diabetes, and obesity. Hyperlipidemia is known as an abnormal lipid metabolism presents in the bloodstream which associated with co-morbidities and mortal...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/116540/ http://psasir.upm.edu.my/id/eprint/116540/1/116540.pdf |
| Summary: | Intervention of herbal combination is widely used to decrease the risk of
metabolic disorders such as cardiovascular diseases, hyperlipidemia, diabetes,
and obesity. Hyperlipidemia is known as an abnormal lipid metabolism presents
in the bloodstream which associated with co-morbidities and mortalities that
grossly rising worldwide. The study aims to investigate the anti-hyperlipidemia
activities of a combination of ZACAH mixture (Zingiber officinale Roscoe
(ginger), Allium sativum L. (garlic), Citrus lemon (L.) Osbeck, Honey, and Malus
domestica Borkh. Cider Vinegar in hyperlipidemic rats. High-performance liquid
chromatography (HPLC) was performed to identify the phytochemical
component that presents in the ZACAH mixture. 36 Sprague dawley (SD) rats
were divided into 6 groups. ZACAH mixture (1ml/kg, 3 ml/kg, 5 ml/kg) was
administered along with a high cholesterol diet (HCD) via oral gavage, daily for
18 weeks. Simvastatin 10 mg/kg was used as a standard drug. At end of week
18, the rats were sacrificed and blood and organs were collected for further
experiments. The blood collection was performed for lipid profile (TC, TG, LDL),
blood toxicity (Creatinine, AST, ALT), and enzymatic activity (HMG-CoA
reductase, LCAT, ACAT2). Besides, liver, kidney and adipose tissue were
performed for histological examination by using hematoxylin & eosin (H&E) stain.
ZACAH mixture had ORAC value 2000 μmol TE/100 mL, TPC (7537 ±
54.5mg/100g GAE), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical
scavenging activity (27.34 ± 2.71%) , Elastase inhibitory assay (29.29 ± 1.65%)
and Lipoxygenase inhibitory assay (98.58 ± 1.42%). The phytochemical
component found in the ZACAH mixture is hesperidin. Bodyweight, total
cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL) reduced and an
increase in high density lipoprotein (HDL) was observed in ZACAH mixture
treated groups compared to the positive control (PC). ZACAH mixture treated
groups reduced HMG-CoA reductase and ACAT2 activity led to a reduction of
TC, TG, LDL and an increase in HDL. Histological examinations revealed that
lipid accumulation was reduced in the liver tissue and the size of adipocytes was
suppressed in the ZACAH mixture treated group as compared to the PC group.
The findings demonstrate that the ZACAH mixture at 5ml/kg BW significantly
(p<0.05) has a strong hyperlipidemia activity that can be an alternative approach
to combat hyperlipidemia treatment. |
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