Modification of lipase by polyethylene glycol
Lipase was modified using polyethylene glycol activated by p-nitrochloroformate. The hydrolytic activity of the polyethylene glycol-derivatised lipase (PEG-lipase) was relatively low compared with that of the unmodified enzyme in aqueous system. The esterification activity, however, was enhanced fol...
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| Format: | Article |
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Informa Healthcare
1991
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116534/ |
| _version_ | 1848867027901480960 |
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| author | Basri, M. Salleh, A.B. Ampon, K. Yunus, W.M.Z. Razak, C.N.A. |
| author_facet | Basri, M. Salleh, A.B. Ampon, K. Yunus, W.M.Z. Razak, C.N.A. |
| author_sort | Basri, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Lipase was modified using polyethylene glycol activated by p-nitrochloroformate. The hydrolytic activity of the polyethylene glycol-derivatised lipase (PEG-lipase) was relatively low compared with that of the unmodified enzyme in aqueous system. The esterification activity, however, was enhanced following the modification. The rate of esterification of butyric acid was higher than that of oleic acid. Benzene was the best solvent for the esterification reaction. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| first_indexed | 2025-11-15T14:29:58Z |
| format | Article |
| id | upm-116534 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:29:58Z |
| publishDate | 1991 |
| publisher | Informa Healthcare |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1165342025-04-10T06:40:07Z http://psasir.upm.edu.my/id/eprint/116534/ Modification of lipase by polyethylene glycol Basri, M. Salleh, A.B. Ampon, K. Yunus, W.M.Z. Razak, C.N.A. Lipase was modified using polyethylene glycol activated by p-nitrochloroformate. The hydrolytic activity of the polyethylene glycol-derivatised lipase (PEG-lipase) was relatively low compared with that of the unmodified enzyme in aqueous system. The esterification activity, however, was enhanced following the modification. The rate of esterification of butyric acid was higher than that of oleic acid. Benzene was the best solvent for the esterification reaction. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. Informa Healthcare 1991 Article PeerReviewed Basri, M. and Salleh, A.B. and Ampon, K. and Yunus, W.M.Z. and Razak, C.N.A. (1991) Modification of lipase by polyethylene glycol. Biocatalysis and Biotransformation, 4 (4). pp. 313-317. ISSN 1024-2422; eISSN: 1024-2422 https://www.tandfonline.com/doi/full/10.3109/10242429109000695 10.3109/10242429109000695 |
| spellingShingle | Basri, M. Salleh, A.B. Ampon, K. Yunus, W.M.Z. Razak, C.N.A. Modification of lipase by polyethylene glycol |
| title | Modification of lipase by polyethylene glycol |
| title_full | Modification of lipase by polyethylene glycol |
| title_fullStr | Modification of lipase by polyethylene glycol |
| title_full_unstemmed | Modification of lipase by polyethylene glycol |
| title_short | Modification of lipase by polyethylene glycol |
| title_sort | modification of lipase by polyethylene glycol |
| url | http://psasir.upm.edu.my/id/eprint/116534/ http://psasir.upm.edu.my/id/eprint/116534/ http://psasir.upm.edu.my/id/eprint/116534/ |