Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection
Leptospirosis is an acute bacterial febrile disease affecting humans and animals in many tropical and subtropical countries. This work presents an optimization of surface-enhanced Raman spectroscopy (SERS) substrates to probe vibrational spectroscopic detail from Leptospira deoxyribonucleic acid (DN...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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SAGE Publications
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116476/ |
| _version_ | 1848867012055400448 |
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| author | Abdul Razak, Anis Athirah Shatar, Liyana Ramli, Aima Kassim, Syara Mohd Ghazali, Mohd Sabri Chee, Hui Yee Zakaria, Rozalina Mahdi, Mohd Adzir Suhailin, Fariza Hanim |
| author_facet | Abdul Razak, Anis Athirah Shatar, Liyana Ramli, Aima Kassim, Syara Mohd Ghazali, Mohd Sabri Chee, Hui Yee Zakaria, Rozalina Mahdi, Mohd Adzir Suhailin, Fariza Hanim |
| author_sort | Abdul Razak, Anis Athirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Leptospirosis is an acute bacterial febrile disease affecting humans and animals in many tropical and subtropical countries. This work presents an optimization of surface-enhanced Raman spectroscopy (SERS) substrates to probe vibrational spectroscopic detail from Leptospira deoxyribonucleic acid (DNA). The pathogenic gene of LipL32 was used as a biomarker. The SERS substrates were based on a photonic crystal (PC) structure embedded with bi-metallic gold and silver nanoparticles (PC@AuAg NPs). The localized plasmonic resonance of AuAg NPs was coupled to the Raman modes of the target through SERS interaction. Prior to detection, the AuAg NPs were functionalized with chemical linkers to facilitate specific conjugation between metallic surfaces and DNA biomolecules. The immobilization and hybridization of probe DNA to their complementary target DNA (cDNA) created duplex formation for detection. The configuration was also tested with non-complementary DNA to verify detection specificity. Prominent SERS peaks were recorded, and the characteristic intensity decreased after cDNA hybridization due to less base interaction after complementary pairing. Distinct SERS behavior from the negative control test was also observed in non-complementary interaction. The configuration is highly attractive and can be potentially extended for sensitive and label-free detection of leptospiral DNA, paving the way for alternative diagnosis of leptospirosis. |
| first_indexed | 2025-11-15T14:29:43Z |
| format | Article |
| id | upm-116476 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:29:43Z |
| publishDate | 2024 |
| publisher | SAGE Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1164762025-07-07T09:11:36Z http://psasir.upm.edu.my/id/eprint/116476/ Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection Abdul Razak, Anis Athirah Shatar, Liyana Ramli, Aima Kassim, Syara Mohd Ghazali, Mohd Sabri Chee, Hui Yee Zakaria, Rozalina Mahdi, Mohd Adzir Suhailin, Fariza Hanim Leptospirosis is an acute bacterial febrile disease affecting humans and animals in many tropical and subtropical countries. This work presents an optimization of surface-enhanced Raman spectroscopy (SERS) substrates to probe vibrational spectroscopic detail from Leptospira deoxyribonucleic acid (DNA). The pathogenic gene of LipL32 was used as a biomarker. The SERS substrates were based on a photonic crystal (PC) structure embedded with bi-metallic gold and silver nanoparticles (PC@AuAg NPs). The localized plasmonic resonance of AuAg NPs was coupled to the Raman modes of the target through SERS interaction. Prior to detection, the AuAg NPs were functionalized with chemical linkers to facilitate specific conjugation between metallic surfaces and DNA biomolecules. The immobilization and hybridization of probe DNA to their complementary target DNA (cDNA) created duplex formation for detection. The configuration was also tested with non-complementary DNA to verify detection specificity. Prominent SERS peaks were recorded, and the characteristic intensity decreased after cDNA hybridization due to less base interaction after complementary pairing. Distinct SERS behavior from the negative control test was also observed in non-complementary interaction. The configuration is highly attractive and can be potentially extended for sensitive and label-free detection of leptospiral DNA, paving the way for alternative diagnosis of leptospirosis. SAGE Publications 2024-12-16 Article PeerReviewed Abdul Razak, Anis Athirah and Shatar, Liyana and Ramli, Aima and Kassim, Syara and Mohd Ghazali, Mohd Sabri and Chee, Hui Yee and Zakaria, Rozalina and Mahdi, Mohd Adzir and Suhailin, Fariza Hanim (2024) Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection. Applied Spectroscopy. ISSN 0003-7028; eISSN: 1943-3530 https://journals.sagepub.com/doi/10.1177/00037028241303780 10.1177/00037028241303780 |
| spellingShingle | Abdul Razak, Anis Athirah Shatar, Liyana Ramli, Aima Kassim, Syara Mohd Ghazali, Mohd Sabri Chee, Hui Yee Zakaria, Rozalina Mahdi, Mohd Adzir Suhailin, Fariza Hanim Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title | Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title_full | Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title_fullStr | Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title_full_unstemmed | Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title_short | Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection |
| title_sort | surface-enhanced raman spectroscopy (sers) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral dna detection |
| url | http://psasir.upm.edu.my/id/eprint/116476/ http://psasir.upm.edu.my/id/eprint/116476/ http://psasir.upm.edu.my/id/eprint/116476/ |