Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking
The Azolla pinnata fern protein was enzymatically hydrolysed with Alcalase, Flavourzyme, and Papain at varying degrees of hydrolysis (10, 20, and 30%) to generate multi-biologically active protein hydrolysates. The extensively hydrolysed (30%) Alcalase-generated hydrolysate (AFPH-AE) was most active...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116419/ http://psasir.upm.edu.my/id/eprint/116419/1/116419.pdf |
| _version_ | 1848867001292816384 |
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| author | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Shamsudin, Rosnah Chau, De Ming Saari, Nazamid |
| author_facet | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Shamsudin, Rosnah Chau, De Ming Saari, Nazamid |
| author_sort | Qoms, Mohammed S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The Azolla pinnata fern protein was enzymatically hydrolysed with Alcalase, Flavourzyme, and Papain at varying degrees of hydrolysis (10, 20, and 30%) to generate multi-biologically active protein hydrolysates. The extensively hydrolysed (30%) Alcalase-generated hydrolysate (AFPH-AE) was most active demonstrating antihypertensive (ACE inhibition), antidiabetic (DPP-IV, α-glucosidase, and α-amylase inhibition), and antioxidant (DPPH, ABTS, and FRAP) activities. AFPH-AE exhibited an uncompetitive inhibition mode against ACE and α-glucosidase, a mixed inhibition mode against α-amylase, and a noncompetitive inhibition mode against DPP-IV. This hydrolysate was highly stable under different food processing conditions including pH 5, 7, and 8, NaCl up to 150 mM, and a high temperature of 100 °C. The low molecular weight fraction (< 3 kDa) exhibited high biological activities, and a total of 15 low molecular weight bioactive peptides were identified. Molecular docking revealed that the peptide-enzyme interactions were mainly mediated via hydrogen bonds and hydrophobic interactions. Overall, these findings reveal the potential of AFPH-AE as a functional and/or nutraceutical ingredient with multifunctional antihypertensive, antidiabetic, and antioxidant effects. |
| first_indexed | 2025-11-15T14:29:33Z |
| format | Article |
| id | upm-116419 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:29:33Z |
| publishDate | 2024 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1164192025-04-09T04:01:55Z http://psasir.upm.edu.my/id/eprint/116419/ Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Shamsudin, Rosnah Chau, De Ming Saari, Nazamid The Azolla pinnata fern protein was enzymatically hydrolysed with Alcalase, Flavourzyme, and Papain at varying degrees of hydrolysis (10, 20, and 30%) to generate multi-biologically active protein hydrolysates. The extensively hydrolysed (30%) Alcalase-generated hydrolysate (AFPH-AE) was most active demonstrating antihypertensive (ACE inhibition), antidiabetic (DPP-IV, α-glucosidase, and α-amylase inhibition), and antioxidant (DPPH, ABTS, and FRAP) activities. AFPH-AE exhibited an uncompetitive inhibition mode against ACE and α-glucosidase, a mixed inhibition mode against α-amylase, and a noncompetitive inhibition mode against DPP-IV. This hydrolysate was highly stable under different food processing conditions including pH 5, 7, and 8, NaCl up to 150 mM, and a high temperature of 100 °C. The low molecular weight fraction (< 3 kDa) exhibited high biological activities, and a total of 15 low molecular weight bioactive peptides were identified. Molecular docking revealed that the peptide-enzyme interactions were mainly mediated via hydrogen bonds and hydrophobic interactions. Overall, these findings reveal the potential of AFPH-AE as a functional and/or nutraceutical ingredient with multifunctional antihypertensive, antidiabetic, and antioxidant effects. Springer 2024-04-27 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116419/1/116419.pdf Qoms, Mohammed S. and Arulrajah, Brisha and Wan Ibadullah, Wan Zunairah and Ramli, Nurul Shazini and Shamsudin, Rosnah and Chau, De Ming and Saari, Nazamid (2024) Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking. Food and Bioprocess Technology, 17 (12). pp. 4806-4823. ISSN 1935-5130; eISSN: 1935-5149 https://link.springer.com/article/10.1007/s11947-024-03412-1?error=cookies_not_supported&code=eb3e3255-970e-4b58-b8cc-34316ec66bc1 10.1007/s11947-024-03412-1 |
| spellingShingle | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Shamsudin, Rosnah Chau, De Ming Saari, Nazamid Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title | Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title_full | Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title_fullStr | Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title_full_unstemmed | Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title_short | Antihypertensive, antidiabetic, and antioxidant properties of novel Azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| title_sort | antihypertensive, antidiabetic, and antioxidant properties of novel azolla pinnata fern protein hydrolysates: inhibition mechanism, stability, profiling, and molecular docking |
| url | http://psasir.upm.edu.my/id/eprint/116419/ http://psasir.upm.edu.my/id/eprint/116419/ http://psasir.upm.edu.my/id/eprint/116419/ http://psasir.upm.edu.my/id/eprint/116419/1/116419.pdf |