Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice
Background: The prevalence of weedy rice infestation has been shown to cause a significant reduction in Malaysian rice yield. The introduction of the Clearfield© Production System has significantly reduce weedy rice infestation, but its mismanagement has led to an increase in imidazolinone-resistant...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Brazilian Weed Science Society
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116417/ http://psasir.upm.edu.my/id/eprint/116417/1/116417.pdf |
| _version_ | 1848867000654233600 |
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| author | Ahmad Hamdani, Muhammad Saiful Zakaria, Norazua Jamil, Mohammad Azizi |
| author_facet | Ahmad Hamdani, Muhammad Saiful Zakaria, Norazua Jamil, Mohammad Azizi |
| author_sort | Ahmad Hamdani, Muhammad Saiful |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: The prevalence of weedy rice infestation has been shown to cause a significant reduction in Malaysian rice yield. The introduction of the Clearfield© Production System has significantly reduce weedy rice infestation, but its mismanagement has led to an increase in imidazolinone-resistant weedy rice. Objective: This study aims to investigate the resistance levels and patterns of weedy rice and to identify possible causes of resistance. Methods: The putative imidazolinone-resistant and susceptible weedy rice were collected in two different rice fields in Kelantan, Malaysia. A petri dish seed bioassay and whole-plant dose-response studies were conducted using three imidazolinone herbicides at seven rates. Surviving plants underwent molecular DNA extraction, nucleotide sequencing, and in vitro acetohydroxy acid synthase (AHAS) inhibition assay studies. Results: The putative imidazolinone-resistant weedy rice was found to be resistant to all three imidazolinone herbicides at the recommended field rate. Further confirmation by DNA sequencing revealed a Ser-653-Asn mutation in all resistant populations. In vitro AHAS inhibition assays confirmed imidazolinone resistance at the enzymatic level. Conclusion: The weedy rice population on the eastern coast of Peninsular Malaysia is resistant to imidazolinone herbicides. The Ser-653-Asn mutation was detected and is known to be the primary cause of imidazolinone resistance in this population of weedy rice. |
| first_indexed | 2025-11-15T14:29:32Z |
| format | Article |
| id | upm-116417 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:29:32Z |
| publishDate | 2024 |
| publisher | Brazilian Weed Science Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1164172025-04-07T09:00:27Z http://psasir.upm.edu.my/id/eprint/116417/ Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice Ahmad Hamdani, Muhammad Saiful Zakaria, Norazua Jamil, Mohammad Azizi Background: The prevalence of weedy rice infestation has been shown to cause a significant reduction in Malaysian rice yield. The introduction of the Clearfield© Production System has significantly reduce weedy rice infestation, but its mismanagement has led to an increase in imidazolinone-resistant weedy rice. Objective: This study aims to investigate the resistance levels and patterns of weedy rice and to identify possible causes of resistance. Methods: The putative imidazolinone-resistant and susceptible weedy rice were collected in two different rice fields in Kelantan, Malaysia. A petri dish seed bioassay and whole-plant dose-response studies were conducted using three imidazolinone herbicides at seven rates. Surviving plants underwent molecular DNA extraction, nucleotide sequencing, and in vitro acetohydroxy acid synthase (AHAS) inhibition assay studies. Results: The putative imidazolinone-resistant weedy rice was found to be resistant to all three imidazolinone herbicides at the recommended field rate. Further confirmation by DNA sequencing revealed a Ser-653-Asn mutation in all resistant populations. In vitro AHAS inhibition assays confirmed imidazolinone resistance at the enzymatic level. Conclusion: The weedy rice population on the eastern coast of Peninsular Malaysia is resistant to imidazolinone herbicides. The Ser-653-Asn mutation was detected and is known to be the primary cause of imidazolinone resistance in this population of weedy rice. Brazilian Weed Science Society 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/116417/1/116417.pdf Ahmad Hamdani, Muhammad Saiful and Zakaria, Norazua and Jamil, Mohammad Azizi (2024) Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice. Advances in Weed Science, 42. pp. 1-10. ISSN 2675-9462 https://awsjournal.org/article/identification-of-ser-653-asn-mutation-conferring-resistance-to-imidazolinone-in-malaysian-weedy-rice/ 10.51694/AdvWeedSci/2024;42:00037 |
| spellingShingle | Ahmad Hamdani, Muhammad Saiful Zakaria, Norazua Jamil, Mohammad Azizi Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title | Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title_full | Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title_fullStr | Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title_full_unstemmed | Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title_short | Identification of SER-653-ASN mutation conferring resistance to imidazolinone in Malaysian weedy rice |
| title_sort | identification of ser-653-asn mutation conferring resistance to imidazolinone in malaysian weedy rice |
| url | http://psasir.upm.edu.my/id/eprint/116417/ http://psasir.upm.edu.my/id/eprint/116417/ http://psasir.upm.edu.my/id/eprint/116417/ http://psasir.upm.edu.my/id/eprint/116417/1/116417.pdf |