Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus)
Streptococcosis outbreaks caused by Streptococcus agalactiae infection in tilapia aquaculture have been consistently reported and associated with high mortality and morbidity leading to significant economic losses. Existing vaccine candidates against Streptococcus spp. are designed for intraperitone...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Press
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116386/ http://psasir.upm.edu.my/id/eprint/116386/1/116386.pdf |
| _version_ | 1848866991616557056 |
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| author | Wong, Kuan Yee Megat Mazhar Khair, Megat Hamzah Song, Adelene Ai Lian Masarudin, Mas Jaffri Loh, Jiun Yan Chong, Chou Min Beardall, John Teo, Michelle Yee Mun In, Lionel Lian Aun |
| author_facet | Wong, Kuan Yee Megat Mazhar Khair, Megat Hamzah Song, Adelene Ai Lian Masarudin, Mas Jaffri Loh, Jiun Yan Chong, Chou Min Beardall, John Teo, Michelle Yee Mun In, Lionel Lian Aun |
| author_sort | Wong, Kuan Yee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Streptococcosis outbreaks caused by Streptococcus agalactiae infection in tilapia aquaculture have been consistently reported and associated with high mortality and morbidity leading to significant economic losses. Existing vaccine candidates against Streptococcus spp. are designed for intraperitoneal injections that are not practical and labor-intensive which have prompted farmers to protect aquatic animals with antibiotics, thus encouraging the emergence of multidrug resistant bacteria. In this study, a live recombinant L. lactis vaccine expressing a 1403 bp surface immunogenic protein (SIP) and a 1100 bp truncated SIP (tSIP) gene was developed and evaluated against S. agalactiae infection in tilapia. Both SIP and tSIP sequences were cloned and transformed into L. lactis. The recombinant L. lactis vaccine was orally administered to juvenile tilapia for a month. Detection of SIP-specific serum IgM in vaccinated groups compared to control groups indicated that recombinant proteins expressed from L. lactis could elicit immunogenic reactions in tilapia. Fish immunized with the tSIP vaccine also showed the highest level of protection compared to other test groups, and the mortality rate was significantly reduced compared to both control groups. The relative percentage of survival (RPS) against S. agalactiae for both SIP and tSIP-vaccinated groups was 50 % and 89 %, respectively, at 14 days post-challenge. Significant up-regulation of IgM, IL-1β, IL-10, TNF-α and IFN-γ were observed at day 34 between the vaccinated and control groups. These results indicated that the recombinant lactococcal tSIP vaccine can elicit both cell-mediated and humoral responses and is recommended as a potential oral vaccine against S. agalactiae infection. Future work will include further in vivo challenge assessments of this vaccine candidate fused with adjuvants to boost immunogenicity levels in tilapia. |
| first_indexed | 2025-11-15T14:29:24Z |
| format | Article |
| id | upm-116386 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:29:24Z |
| publishDate | 2024 |
| publisher | Academic Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1163862025-03-28T01:33:11Z http://psasir.upm.edu.my/id/eprint/116386/ Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) Wong, Kuan Yee Megat Mazhar Khair, Megat Hamzah Song, Adelene Ai Lian Masarudin, Mas Jaffri Loh, Jiun Yan Chong, Chou Min Beardall, John Teo, Michelle Yee Mun In, Lionel Lian Aun Streptococcosis outbreaks caused by Streptococcus agalactiae infection in tilapia aquaculture have been consistently reported and associated with high mortality and morbidity leading to significant economic losses. Existing vaccine candidates against Streptococcus spp. are designed for intraperitoneal injections that are not practical and labor-intensive which have prompted farmers to protect aquatic animals with antibiotics, thus encouraging the emergence of multidrug resistant bacteria. In this study, a live recombinant L. lactis vaccine expressing a 1403 bp surface immunogenic protein (SIP) and a 1100 bp truncated SIP (tSIP) gene was developed and evaluated against S. agalactiae infection in tilapia. Both SIP and tSIP sequences were cloned and transformed into L. lactis. The recombinant L. lactis vaccine was orally administered to juvenile tilapia for a month. Detection of SIP-specific serum IgM in vaccinated groups compared to control groups indicated that recombinant proteins expressed from L. lactis could elicit immunogenic reactions in tilapia. Fish immunized with the tSIP vaccine also showed the highest level of protection compared to other test groups, and the mortality rate was significantly reduced compared to both control groups. The relative percentage of survival (RPS) against S. agalactiae for both SIP and tSIP-vaccinated groups was 50 % and 89 %, respectively, at 14 days post-challenge. Significant up-regulation of IgM, IL-1β, IL-10, TNF-α and IFN-γ were observed at day 34 between the vaccinated and control groups. These results indicated that the recombinant lactococcal tSIP vaccine can elicit both cell-mediated and humoral responses and is recommended as a potential oral vaccine against S. agalactiae infection. Future work will include further in vivo challenge assessments of this vaccine candidate fused with adjuvants to boost immunogenicity levels in tilapia. Academic Press 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116386/1/116386.pdf Wong, Kuan Yee and Megat Mazhar Khair, Megat Hamzah and Song, Adelene Ai Lian and Masarudin, Mas Jaffri and Loh, Jiun Yan and Chong, Chou Min and Beardall, John and Teo, Michelle Yee Mun and In, Lionel Lian Aun (2024) Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus). Fish and Shellfish Immunology, 149. art. no. 109572. pp. 1-9. ISSN 1050-4648; eISSN: 1095-9947 https://linkinghub.elsevier.com/retrieve/pii/S1050464824002171 10.1016/j.fsi.2024.109572 |
| spellingShingle | Wong, Kuan Yee Megat Mazhar Khair, Megat Hamzah Song, Adelene Ai Lian Masarudin, Mas Jaffri Loh, Jiun Yan Chong, Chou Min Beardall, John Teo, Michelle Yee Mun In, Lionel Lian Aun Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title | Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title_full | Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title_fullStr | Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title_full_unstemmed | Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title_short | Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus) |
| title_sort | recombinant lactococcal-based oral vaccine for protection against streptococcus agalactiae infections in tilapia (oreochromis niloticus) |
| url | http://psasir.upm.edu.my/id/eprint/116386/ http://psasir.upm.edu.my/id/eprint/116386/ http://psasir.upm.edu.my/id/eprint/116386/ http://psasir.upm.edu.my/id/eprint/116386/1/116386.pdf |