Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum
An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19...
| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116287/ |
| _version_ | 1848866969012404224 |
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| author | A.R., Raha Ross, E. Yusoff, K. Manap, M.Y. Ideris, A. |
| author_facet | A.R., Raha Ross, E. Yusoff, K. Manap, M.Y. Ideris, A. |
| author_sort | A.R., Raha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19 to form pAJ02, which can replicate in Escherichia coli XLI-Blue as well as L. lactis MG1363. The plasmid was stably maintained in Lactococcus for more than 100 generations. |
| first_indexed | 2025-11-15T14:29:02Z |
| format | Article |
| id | upm-116287 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:29:02Z |
| publishDate | 2002 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1162872025-03-25T02:12:43Z http://psasir.upm.edu.my/id/eprint/116287/ Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum A.R., Raha Ross, E. Yusoff, K. Manap, M.Y. Ideris, A. An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19 to form pAJ02, which can replicate in Escherichia coli XLI-Blue as well as L. lactis MG1363. The plasmid was stably maintained in Lactococcus for more than 100 generations. 2002 Article PeerReviewed A.R., Raha and Ross, E. and Yusoff, K. and Manap, M.Y. and Ideris, A. (2002) Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum. Journal of Biochemistry, Molecular Biology and Biophysics, 6 (1). pp. 7-11. ISSN 1025-8140; eISSN: 1025-8140 https://taylorandfrancis.com/ 10.1080/10258140290010151 |
| spellingShingle | A.R., Raha Ross, E. Yusoff, K. Manap, M.Y. Ideris, A. Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title | Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title_full | Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title_fullStr | Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title_full_unstemmed | Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title_short | Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum |
| title_sort | characterisation and molecular cloning of an erythromycin resistance plasmid of lactococcus lactis isolated from chicken cecum |
| url | http://psasir.upm.edu.my/id/eprint/116287/ http://psasir.upm.edu.my/id/eprint/116287/ http://psasir.upm.edu.my/id/eprint/116287/ |