Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia
BACKGROUND: As climate change raises global temperatures, there remains a notable gap in understanding the body’s mechanisms of heat stress defense exhibited by Heat Shock Protein (HSP) within the populations. OBJECTIVE: This study aims to investigate the expression level of HSP70 in response to in...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2025
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116235/ http://psasir.upm.edu.my/id/eprint/116235/1/116235.pdf |
| _version_ | 1848866955657740288 |
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| author | Muhamad, Siti Nurfahirah Md Akim, Abdah Lim, Fang Lee Karuppiah, Karmegam Mohd Shabri, Nur Shabrina Azreen How, Vivien |
| author_facet | Muhamad, Siti Nurfahirah Md Akim, Abdah Lim, Fang Lee Karuppiah, Karmegam Mohd Shabri, Nur Shabrina Azreen How, Vivien |
| author_sort | Muhamad, Siti Nurfahirah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: As climate change raises global temperatures, there remains a notable gap in understanding the body’s mechanisms of heat stress defense exhibited by Heat Shock Protein (HSP) within the populations.
OBJECTIVE: This study aims to investigate the expression level of HSP70 in response to indoor heat exposure among vulnerable populations in both urban and rural settings.
METHODS: A comparative cross-sectional was conducted among 108 participants from urban and rural areas in Klang Valley, Malaysia. The study included face-to-face interviews, indoor heat exposure monitoring, and thermal stress classification using the Universal Thermal Climate Index (UTCI). HSP70 gene and protein expressions were analyzed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and HSP70 High Sensitivity Enzyme-linked Immunosorbent Assay (ELISA), respectively. RESULTS: Urban areas experienced signficantly higher UTCI heat exposure levels than rural areas (p < 0.001). In response to heat stress, vulnerable populations in urban areas exhibited higher HSP70 gene relative expression and HSP70 protein expression. A significant mean difference in the plasma HSP70 protein expression was observed between the two groups (p < 0.001). The linear mixed model (LMM) revealed a significant association between UTCI heat exposure levels and HSP70 gene and protein expression in both groups (p < 0.001). |
| first_indexed | 2025-11-15T14:28:50Z |
| format | Article |
| id | upm-116235 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:28:50Z |
| publishDate | 2025 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1162352025-03-24T03:15:05Z http://psasir.upm.edu.my/id/eprint/116235/ Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia Muhamad, Siti Nurfahirah Md Akim, Abdah Lim, Fang Lee Karuppiah, Karmegam Mohd Shabri, Nur Shabrina Azreen How, Vivien BACKGROUND: As climate change raises global temperatures, there remains a notable gap in understanding the body’s mechanisms of heat stress defense exhibited by Heat Shock Protein (HSP) within the populations. OBJECTIVE: This study aims to investigate the expression level of HSP70 in response to indoor heat exposure among vulnerable populations in both urban and rural settings. METHODS: A comparative cross-sectional was conducted among 108 participants from urban and rural areas in Klang Valley, Malaysia. The study included face-to-face interviews, indoor heat exposure monitoring, and thermal stress classification using the Universal Thermal Climate Index (UTCI). HSP70 gene and protein expressions were analyzed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and HSP70 High Sensitivity Enzyme-linked Immunosorbent Assay (ELISA), respectively. RESULTS: Urban areas experienced signficantly higher UTCI heat exposure levels than rural areas (p < 0.001). In response to heat stress, vulnerable populations in urban areas exhibited higher HSP70 gene relative expression and HSP70 protein expression. A significant mean difference in the plasma HSP70 protein expression was observed between the two groups (p < 0.001). The linear mixed model (LMM) revealed a significant association between UTCI heat exposure levels and HSP70 gene and protein expression in both groups (p < 0.001). Springer Nature 2025-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/116235/1/116235.pdf Muhamad, Siti Nurfahirah and Md Akim, Abdah and Lim, Fang Lee and Karuppiah, Karmegam and Mohd Shabri, Nur Shabrina Azreen and How, Vivien (2025) Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia. Journal of Exposure Science and Environmental Epidemiology. art. no. 4255. pp. 1-9. ISSN 1559-0631; eISSN: 1559-064X (In Press) https://www.nature.com/articles/s41370-025-00764-4 10.1038/s41370-025-00764-4 |
| spellingShingle | Muhamad, Siti Nurfahirah Md Akim, Abdah Lim, Fang Lee Karuppiah, Karmegam Mohd Shabri, Nur Shabrina Azreen How, Vivien Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title | Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title_full | Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title_fullStr | Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title_full_unstemmed | Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title_short | Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia |
| title_sort | heat stress-induced heat shock protein 70 (hsp70) expressions among vulnerable populations in urban and rural areas klang valley, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/116235/ http://psasir.upm.edu.my/id/eprint/116235/ http://psasir.upm.edu.my/id/eprint/116235/ http://psasir.upm.edu.my/id/eprint/116235/1/116235.pdf |