A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects
Regular testing and systematic investigation play a vital role to ensure product safety. Until now, the existing food authentication techniques have been based on proteins, lipids, and nucleic acid-based assays. Among various deoxyribonucleic acid (DNA)-based methods, the recently developed Clustere...
| Main Authors: | , , , , |
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| Format: | Article |
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Taylor and Francis
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116213/ |
| _version_ | 1848866950536495104 |
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| author | Sultana, Sharmin Azlan, Azrina Mohd Desa, Mohd Nasir Mahyudin, Nor Ainy Anburaj, Amaladoss |
| author_facet | Sultana, Sharmin Azlan, Azrina Mohd Desa, Mohd Nasir Mahyudin, Nor Ainy Anburaj, Amaladoss |
| author_sort | Sultana, Sharmin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Regular testing and systematic investigation play a vital role to ensure product safety. Until now, the existing food authentication techniques have been based on proteins, lipids, and nucleic acid-based assays. Among various deoxyribonucleic acid (DNA)-based methods, the recently developed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) based bio-sensing is an innovative and fast-expanding technology. The CRISPR/Cas-9 is known as Clustered Regularly Interspaced Short Palindromic Repeats due to the flexibility and simplicity of the CRISPR/Cas9 site-specific editing tool has been applied in many biological research areas such as Gene therapy, cell line development, discovering mechanisms of disease, and drug discovery. Nowadays, the CRISPR-Cas system has also been introduced into food authentication via detecting DNA barcodes of poultry and livestock both in processed and unprocessed food samples. This review documents various DNA based approaches, in an accessible format. Future CRISPR technologies are forecast while challenges are outlined. |
| first_indexed | 2025-11-15T14:28:45Z |
| format | Article |
| id | upm-116213 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:28:45Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1162132025-04-21T03:17:24Z http://psasir.upm.edu.my/id/eprint/116213/ A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects Sultana, Sharmin Azlan, Azrina Mohd Desa, Mohd Nasir Mahyudin, Nor Ainy Anburaj, Amaladoss Regular testing and systematic investigation play a vital role to ensure product safety. Until now, the existing food authentication techniques have been based on proteins, lipids, and nucleic acid-based assays. Among various deoxyribonucleic acid (DNA)-based methods, the recently developed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) based bio-sensing is an innovative and fast-expanding technology. The CRISPR/Cas-9 is known as Clustered Regularly Interspaced Short Palindromic Repeats due to the flexibility and simplicity of the CRISPR/Cas9 site-specific editing tool has been applied in many biological research areas such as Gene therapy, cell line development, discovering mechanisms of disease, and drug discovery. Nowadays, the CRISPR-Cas system has also been introduced into food authentication via detecting DNA barcodes of poultry and livestock both in processed and unprocessed food samples. This review documents various DNA based approaches, in an accessible format. Future CRISPR technologies are forecast while challenges are outlined. Taylor and Francis 2024-01-29 Article PeerReviewed Sultana, Sharmin and Azlan, Azrina and Mohd Desa, Mohd Nasir and Mahyudin, Nor Ainy and Anburaj, Amaladoss (2024) A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects. Food Additives and Contaminants: Part A, 41 (3). pp. 213-227. ISSN 1944-0049; eISSN: 1944-0057 https://www.tandfonline.com/doi/full/10.1080/19440049.2024.2304577 10.1080/19440049.2024.2304577 |
| spellingShingle | Sultana, Sharmin Azlan, Azrina Mohd Desa, Mohd Nasir Mahyudin, Nor Ainy Anburaj, Amaladoss A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title | A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title_full | A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title_fullStr | A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title_full_unstemmed | A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title_short | A review of CRISPR-Cas and PCR-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| title_sort | review of crispr-cas and pcr-based methods for the detection of animal species in the food chain-current challenges and future prospects |
| url | http://psasir.upm.edu.my/id/eprint/116213/ http://psasir.upm.edu.my/id/eprint/116213/ http://psasir.upm.edu.my/id/eprint/116213/ |