Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus
Depending on their chemical structure and geochemistry, clay minerals can display potent antibacterial properties against a range of bacterial pathogens. Malaysian Carey clay was evaluated for its antibacterial activity against food-borne Staphylococcus aureus ATCC 13565 strains. The minimum inhibit...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Taylor and Francis
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116192/ |
| _version_ | 1848866945643839488 |
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| author | Azmi, Nur Naqiyah Mahyudin, Nor Ainy Wan Omar, Wan Hasyera Abdullah, Abdul Halim Ismail, Roslan Ishak, Che Fauziah Sharples, Gary J. |
| author_facet | Azmi, Nur Naqiyah Mahyudin, Nor Ainy Wan Omar, Wan Hasyera Abdullah, Abdul Halim Ismail, Roslan Ishak, Che Fauziah Sharples, Gary J. |
| author_sort | Azmi, Nur Naqiyah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Depending on their chemical structure and geochemistry, clay minerals can display potent antibacterial properties against a range of bacterial pathogens. Malaysian Carey clay was evaluated for its antibacterial activity against food-borne Staphylococcus aureus ATCC 13565 strains. The minimum inhibitory concentration (MIC) and minimum bactericidal activity (MBC) of both Carey clay leachates and suspension were 125 mg/mL and 250 mg/mL, respectively. Time-kill assay revealed that 2x MIC and 4x MIC Carey clay in both leachate and suspension forms resulted in complete killing of S. aureus. Antibacterial mechanism was investigated through imaging of bacterial morphology using TEM and determination of reactive oxygen species (ROS) using NBT assay. Imaging of bacterial morphology using TEM showed abnormalities, including disrupted cell walls following exposure to Carey clay, and the antibacterial activity was associated with generation of ROS. Our study suggests that Carey clay displays promising functionality as a natural antibacterial agent in the food industry. |
| first_indexed | 2025-11-15T14:28:40Z |
| format | Article |
| id | upm-116192 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:28:40Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1161922025-04-21T03:16:17Z http://psasir.upm.edu.my/id/eprint/116192/ Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus Azmi, Nur Naqiyah Mahyudin, Nor Ainy Wan Omar, Wan Hasyera Abdullah, Abdul Halim Ismail, Roslan Ishak, Che Fauziah Sharples, Gary J. Depending on their chemical structure and geochemistry, clay minerals can display potent antibacterial properties against a range of bacterial pathogens. Malaysian Carey clay was evaluated for its antibacterial activity against food-borne Staphylococcus aureus ATCC 13565 strains. The minimum inhibitory concentration (MIC) and minimum bactericidal activity (MBC) of both Carey clay leachates and suspension were 125 mg/mL and 250 mg/mL, respectively. Time-kill assay revealed that 2x MIC and 4x MIC Carey clay in both leachate and suspension forms resulted in complete killing of S. aureus. Antibacterial mechanism was investigated through imaging of bacterial morphology using TEM and determination of reactive oxygen species (ROS) using NBT assay. Imaging of bacterial morphology using TEM showed abnormalities, including disrupted cell walls following exposure to Carey clay, and the antibacterial activity was associated with generation of ROS. Our study suggests that Carey clay displays promising functionality as a natural antibacterial agent in the food industry. Taylor and Francis 2024-05-20 Article PeerReviewed Azmi, Nur Naqiyah and Mahyudin, Nor Ainy and Wan Omar, Wan Hasyera and Abdullah, Abdul Halim and Ismail, Roslan and Ishak, Che Fauziah and Sharples, Gary J. (2024) Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus. Natural Product Research. pp. 1-5. ISSN 1478-6419; eISSN: 1478-6427 https://www.tandfonline.com/doi/full/10.1080/14786419.2024.2355583 10.1080/14786419.2024.2355583 |
| spellingShingle | Azmi, Nur Naqiyah Mahyudin, Nor Ainy Wan Omar, Wan Hasyera Abdullah, Abdul Halim Ismail, Roslan Ishak, Che Fauziah Sharples, Gary J. Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title | Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title_full | Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title_fullStr | Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title_full_unstemmed | Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title_short | Antibacterial mechanism of Malaysian Carey clay against food-borne Staphylococcus aureus |
| title_sort | antibacterial mechanism of malaysian carey clay against food-borne staphylococcus aureus |
| url | http://psasir.upm.edu.my/id/eprint/116192/ http://psasir.upm.edu.my/id/eprint/116192/ http://psasir.upm.edu.my/id/eprint/116192/ |