Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh)
Nisin is an effective biopreservative, however its wide application in food is restricted due to its low productivity. Therefore, the present work aimed to increase its yield by disrupting lactate dehydrogenase (ldh) and re-directing the carbon flow towards nisin production. The ldh-deficient Lactoc...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Taylor and Francis
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/116170/ |
| _version_ | 1848866940355870720 |
|---|---|
| author | Mohd Rasid, Nur Hidayah Abdul Halid, Nadrah Ai-Lian, Adelene Song Sabri, Suriana Abd Rahim, Muhamad Hafiz Saari, Nazamid Hasan, Hanan |
| author_facet | Mohd Rasid, Nur Hidayah Abdul Halid, Nadrah Ai-Lian, Adelene Song Sabri, Suriana Abd Rahim, Muhamad Hafiz Saari, Nazamid Hasan, Hanan |
| author_sort | Mohd Rasid, Nur Hidayah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nisin is an effective biopreservative, however its wide application in food is restricted due to its low productivity. Therefore, the present work aimed to increase its yield by disrupting lactate dehydrogenase (ldh) and re-directing the carbon flow towards nisin production. The ldh-deficient Lactococcus lactis ATCC 11454 (ldhΔ) strain was created by inserting ampicillin (amp) within the ldh to disrupt its function. Under controlled fermentation conditions (30°C, pH 6, 1.0% glucose), the ldhΔ strain exhibited negligible decrease in pH (2.5%) over 48 h, in contrast to the wild-type (WT) strain, which experienced a 23.6% decrease in pH (pH 4.2). Overall, the ldhΔ strain showed a significant (p < 0.05) increase in growth and nisin activity by 24.9% and 62% compared to the WT strain, respectively. This strategy could serve as a practical approach to further enhance the production of bacteriocin or other compounds by modulating their metabolic pathways. |
| first_indexed | 2025-11-15T14:28:35Z |
| format | Article |
| id | upm-116170 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:28:35Z |
| publishDate | 2024 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1161702025-04-21T03:14:33Z http://psasir.upm.edu.my/id/eprint/116170/ Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) Mohd Rasid, Nur Hidayah Abdul Halid, Nadrah Ai-Lian, Adelene Song Sabri, Suriana Abd Rahim, Muhamad Hafiz Saari, Nazamid Hasan, Hanan Nisin is an effective biopreservative, however its wide application in food is restricted due to its low productivity. Therefore, the present work aimed to increase its yield by disrupting lactate dehydrogenase (ldh) and re-directing the carbon flow towards nisin production. The ldh-deficient Lactococcus lactis ATCC 11454 (ldhΔ) strain was created by inserting ampicillin (amp) within the ldh to disrupt its function. Under controlled fermentation conditions (30°C, pH 6, 1.0% glucose), the ldhΔ strain exhibited negligible decrease in pH (2.5%) over 48 h, in contrast to the wild-type (WT) strain, which experienced a 23.6% decrease in pH (pH 4.2). Overall, the ldhΔ strain showed a significant (p < 0.05) increase in growth and nisin activity by 24.9% and 62% compared to the WT strain, respectively. This strategy could serve as a practical approach to further enhance the production of bacteriocin or other compounds by modulating their metabolic pathways. Taylor and Francis 2024-06-06 Article PeerReviewed Mohd Rasid, Nur Hidayah and Abdul Halid, Nadrah and Ai-Lian, Adelene Song and Sabri, Suriana and Abd Rahim, Muhamad Hafiz and Saari, Nazamid and Hasan, Hanan (2024) Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh). Food Biotechnology, 38 (2). pp. 180-194. ISSN 0890-5436; eISSN: 1532-4249 https://www.tandfonline.com/doi/full/10.1080/08905436.2024.2345772 10.1080/08905436.2024.2345772 |
| spellingShingle | Mohd Rasid, Nur Hidayah Abdul Halid, Nadrah Ai-Lian, Adelene Song Sabri, Suriana Abd Rahim, Muhamad Hafiz Saari, Nazamid Hasan, Hanan Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title | Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title_full | Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title_fullStr | Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title_full_unstemmed | Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title_short | Rerouting the metabolic pathway in Lactococcus lactis ATCC 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| title_sort | rerouting the metabolic pathway in lactococcus lactis atcc 11454 to improve nisin productivity by disrupting lactate dehydrogenase (ldh) |
| url | http://psasir.upm.edu.my/id/eprint/116170/ http://psasir.upm.edu.my/id/eprint/116170/ http://psasir.upm.edu.my/id/eprint/116170/ |