In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes
The aim of the study was to investigate the regulatory effects of mixed antioxidants, including tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes, on inflammatory response and epidermal integrity of normal human epidermal keratinocytes (NHEK) stimulated with cytokines to mimic ato...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116103/ http://psasir.upm.edu.my/id/eprint/116103/1/116103.pdf |
| _version_ | 1848866928780640256 |
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| author | Gan, Yee-Lin Tan, Chin-Ping Cheah, Yoke-Kqueen Ariffina, Hidayah Wasoh, Helmi Lai, Oi-Ming |
| author_facet | Gan, Yee-Lin Tan, Chin-Ping Cheah, Yoke-Kqueen Ariffina, Hidayah Wasoh, Helmi Lai, Oi-Ming |
| author_sort | Gan, Yee-Lin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of the study was to investigate the regulatory effects of mixed antioxidants, including tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes, on inflammatory response and epidermal integrity of normal human epidermal keratinocytes (NHEK) stimulated with cytokines to mimic atopic dermatitis (AD). Nano encapsulated mixed antioxidants (NMA) were prepared by encapsulating tocotrienol-rich fraction, ascorbyl tetraisopalmitate and carotenes into nanoemulsion. The interleukin (IL)-4 (50 ng/mL), interferon (IFN)-γ (50 ng/mL), and tumor necrosis factor (TNF)-α (20 ng/mL) individually stimulated NHEK cells were treated with NMA (2.1-2100 ng/mL). Then, cytokines, chemokines and structural proteins expressions were determined. Cell monolayer integrity was assessed using dispase-based keratinocyte dissociation and wound-healing assays.The key findings of the study showed that NMA treatment (2100 ng/mL) resulted in a complete abolishment of upmodulation thymic stromal lymphopoietin (TSLP) expression induced by IL-4, IFN-γ, and TNF-α. Upregulated expression of thymus and activation regulated chemokine (TARC) following IL-4 stimulation was attenuated by NMA (2.1-2100 ng/mL). The increment of interferon gamma-induced protein 10 (IP-10) and monocyte chemoattractant protein-1 (MCP-1) expressions resulting from IL-4, IFN-γ, and TNF-α stimulations was significantly suppressed by NMA treatment. The downregulated filaggrin expression provoked by IL-4 and TNF-α stimulations was abrogated with NMA treatment (2.1-2100 ng/mL). Treatment with NMA significantly improved the production of cornified envelope proteins, decreased the dissociation scores and accelerated wound healing of IL-4, IFN-γ, and TNF-α stimulated NHEK. |
| first_indexed | 2025-11-15T14:28:24Z |
| format | Article |
| id | upm-116103 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:28:24Z |
| publishDate | 2024 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1161032025-03-19T05:23:05Z http://psasir.upm.edu.my/id/eprint/116103/ In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes Gan, Yee-Lin Tan, Chin-Ping Cheah, Yoke-Kqueen Ariffina, Hidayah Wasoh, Helmi Lai, Oi-Ming The aim of the study was to investigate the regulatory effects of mixed antioxidants, including tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes, on inflammatory response and epidermal integrity of normal human epidermal keratinocytes (NHEK) stimulated with cytokines to mimic atopic dermatitis (AD). Nano encapsulated mixed antioxidants (NMA) were prepared by encapsulating tocotrienol-rich fraction, ascorbyl tetraisopalmitate and carotenes into nanoemulsion. The interleukin (IL)-4 (50 ng/mL), interferon (IFN)-γ (50 ng/mL), and tumor necrosis factor (TNF)-α (20 ng/mL) individually stimulated NHEK cells were treated with NMA (2.1-2100 ng/mL). Then, cytokines, chemokines and structural proteins expressions were determined. Cell monolayer integrity was assessed using dispase-based keratinocyte dissociation and wound-healing assays.The key findings of the study showed that NMA treatment (2100 ng/mL) resulted in a complete abolishment of upmodulation thymic stromal lymphopoietin (TSLP) expression induced by IL-4, IFN-γ, and TNF-α. Upregulated expression of thymus and activation regulated chemokine (TARC) following IL-4 stimulation was attenuated by NMA (2.1-2100 ng/mL). The increment of interferon gamma-induced protein 10 (IP-10) and monocyte chemoattractant protein-1 (MCP-1) expressions resulting from IL-4, IFN-γ, and TNF-α stimulations was significantly suppressed by NMA treatment. The downregulated filaggrin expression provoked by IL-4 and TNF-α stimulations was abrogated with NMA treatment (2.1-2100 ng/mL). Treatment with NMA significantly improved the production of cornified envelope proteins, decreased the dissociation scores and accelerated wound healing of IL-4, IFN-γ, and TNF-α stimulated NHEK. Malaysian Society for Molecular Biology and Biotechnology 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/116103/1/116103.pdf Gan, Yee-Lin and Tan, Chin-Ping and Cheah, Yoke-Kqueen and Ariffina, Hidayah and Wasoh, Helmi and Lai, Oi-Ming (2024) In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes. Asia Pacific Journal of Molecular Biology and Biotechnology, 32 (4). pp. 11-26. ISSN 2672-7277 https://www.msmbb.my/images/publication/volume_32/issue_4/special/02-Gan-et-al.pdf 10.35118/apjmbb.2024.032.4(special).02 |
| spellingShingle | Gan, Yee-Lin Tan, Chin-Ping Cheah, Yoke-Kqueen Ariffina, Hidayah Wasoh, Helmi Lai, Oi-Ming In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title | In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title_full | In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title_fullStr | In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title_full_unstemmed | In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title_short | In vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| title_sort | in vitro modulatory effect of nanoencapsulated tocotrienol-rich fraction, ascorbyl tetraisopalmitate, and carotenes on atopic dermatitis-like model of human epidermal keratinocytes |
| url | http://psasir.upm.edu.my/id/eprint/116103/ http://psasir.upm.edu.my/id/eprint/116103/ http://psasir.upm.edu.my/id/eprint/116103/ http://psasir.upm.edu.my/id/eprint/116103/1/116103.pdf |