Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages
Osteoporosis is a bone disorder caused by an imbalance in the bone remodelling process, specifically between osteoblastogenesis and osteoclastogenesis, and is associated with increased oxidative stress. This study aims to investigate the antioxidant activity of oil palm leaf extracts (OPLEs) and the...
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2024
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| Online Access: | http://psasir.upm.edu.my/id/eprint/116092/ http://psasir.upm.edu.my/id/eprint/116092/1/116092.pdf |
| _version_ | 1848866925762838528 |
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| author | Safa, Parastoo Ismail, Patimah Md Noor, Sabariah Abd Manan, Nizar |
| author_facet | Safa, Parastoo Ismail, Patimah Md Noor, Sabariah Abd Manan, Nizar |
| author_sort | Safa, Parastoo |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Osteoporosis is a bone disorder caused by an imbalance in the bone remodelling process, specifically between osteoblastogenesis and osteoclastogenesis, and is associated with increased oxidative stress. This study aims to investigate the antioxidant activity of oil palm leaf extracts (OPLEs) and their effects on osteoclastogenesis in murine macrophages (RAW 264.7), comparing the results with those of vitamin C (VC). Methanol extract of oil palm leaves (MEOPL) demonstrated the highest total phenolic content (TPC) at 284.26 mg GAE/g dry weight. The antioxidant activity, assessed via DPPH scavenging and FRAP assays, showed that MEOPL had a DPPH inhibition rate of 89.41% and a FRAP value of 105.67%. In cytotoxicity assays, MEOPL-treated cells exhibited significantly higher viability compared to VC-treated cells, with viability percentages exceeding 60% at concentrations up to 0.625 mg/mL. MEOPL also significantly reduced osteoclastogenesis, as indicated by a dose-dependent decrease in TRAP-positive multinucleated cells and a notable reduction in RANKL gene expression. These findings suggest that MEOPL possesses superior anti-osteoclastogenic properties compared to VC and holds promise as a potential therapeutic agent for the prevention and treatment of osteoporosis. However, as this study utilised an in vitro model, the direct translation of these results to clinical scenarios is limited, and further in vivo studies are necessary to confirm the clinical relevance of MEOPL. |
| first_indexed | 2025-11-15T14:28:21Z |
| format | Article |
| id | upm-116092 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T14:28:21Z |
| publishDate | 2024 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
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| spelling | upm-1160922025-03-19T04:05:25Z http://psasir.upm.edu.my/id/eprint/116092/ Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages Safa, Parastoo Ismail, Patimah Md Noor, Sabariah Abd Manan, Nizar Osteoporosis is a bone disorder caused by an imbalance in the bone remodelling process, specifically between osteoblastogenesis and osteoclastogenesis, and is associated with increased oxidative stress. This study aims to investigate the antioxidant activity of oil palm leaf extracts (OPLEs) and their effects on osteoclastogenesis in murine macrophages (RAW 264.7), comparing the results with those of vitamin C (VC). Methanol extract of oil palm leaves (MEOPL) demonstrated the highest total phenolic content (TPC) at 284.26 mg GAE/g dry weight. The antioxidant activity, assessed via DPPH scavenging and FRAP assays, showed that MEOPL had a DPPH inhibition rate of 89.41% and a FRAP value of 105.67%. In cytotoxicity assays, MEOPL-treated cells exhibited significantly higher viability compared to VC-treated cells, with viability percentages exceeding 60% at concentrations up to 0.625 mg/mL. MEOPL also significantly reduced osteoclastogenesis, as indicated by a dose-dependent decrease in TRAP-positive multinucleated cells and a notable reduction in RANKL gene expression. These findings suggest that MEOPL possesses superior anti-osteoclastogenic properties compared to VC and holds promise as a potential therapeutic agent for the prevention and treatment of osteoporosis. However, as this study utilised an in vitro model, the direct translation of these results to clinical scenarios is limited, and further in vivo studies are necessary to confirm the clinical relevance of MEOPL. Hibiscus Publisher 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/116092/1/116092.pdf Safa, Parastoo and Ismail, Patimah and Md Noor, Sabariah and Abd Manan, Nizar (2024) Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages. Journal of Biochemistry, Microbiology and Biotechnology, 12 (2). pp. 24-32. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/1014 10.54987/jobimb.v12i2.1014 |
| spellingShingle | Safa, Parastoo Ismail, Patimah Md Noor, Sabariah Abd Manan, Nizar Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title | Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title_full | Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title_fullStr | Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title_full_unstemmed | Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title_short | Inhibitory effects of oil palm leaf extract on osteoclastogenesis in RAW 264.7 macrophages |
| title_sort | inhibitory effects of oil palm leaf extract on osteoclastogenesis in raw 264.7 macrophages |
| url | http://psasir.upm.edu.my/id/eprint/116092/ http://psasir.upm.edu.my/id/eprint/116092/ http://psasir.upm.edu.my/id/eprint/116092/ http://psasir.upm.edu.my/id/eprint/116092/1/116092.pdf |