Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells
This study investigated the dual potential of Azolla pinnata fern protein hydrolysates (AFPHs) as functional and nutraceutical ingredients in an oil/water emulsion system. The AFPH-stabilised emulsion (AFPH-E) displayed a small and uniform droplet distribution and was stable to aggregation and cream...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Royal Society of Chemistry
2024
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/116073/ |
| _version_ | 1848866921725820928 |
|---|---|
| author | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Chau, De-Ming Sarbini, Shahrul Razid Saari, Nazamid |
| author_facet | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Chau, De-Ming Sarbini, Shahrul Razid Saari, Nazamid |
| author_sort | Qoms, Mohammed S. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study investigated the dual potential of Azolla pinnata fern protein hydrolysates (AFPHs) as functional and nutraceutical ingredients in an oil/water emulsion system. The AFPH-stabilised emulsion (AFPH-E) displayed a small and uniform droplet distribution and was stable to aggregation and creaming over a wide range of pH (5-8), salt concentrations ≤ 100 mM, and heat treatment ≤ 70 °C. Besides, the AFPH-E possessed and maintained strong biological activities, including antihypertensive, antidiabetic, and antioxidant, under different food processing conditions (pH 5-8; NaCl: 50-150 mM, and heat treatment: 30-100 °C). Following in vitro gastrointestinal digestion, the antihypertensive and antioxidant activities were unchanged, while a notable increase of 8% was observed for DPPH. However, the antidiabetic activities were partially reduced in the range of 5-11%. Notably, AFPH-E modulated the gut microbiota and short-chain fatty acids (SCFAs), promoting the growth of beneficial bacteria, particularly Bifidobacteria and Lactobacilli, along with increased SCFA acetate, propionate, and butyrate. Also, AFPH-E up to 10 mg mL−1 did not affect the proliferation of the normal colon cells. In the current work, AFPH demonstrated dual functionality as a plant-based emulsifier with strong biological activities in an oil/water emulsion system and promoted healthy changes in the human gut microbiota. |
| first_indexed | 2025-11-15T14:28:17Z |
| format | Article |
| id | upm-116073 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T14:28:17Z |
| publishDate | 2024 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-1160732025-04-21T03:08:08Z http://psasir.upm.edu.my/id/eprint/116073/ Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Chau, De-Ming Sarbini, Shahrul Razid Saari, Nazamid This study investigated the dual potential of Azolla pinnata fern protein hydrolysates (AFPHs) as functional and nutraceutical ingredients in an oil/water emulsion system. The AFPH-stabilised emulsion (AFPH-E) displayed a small and uniform droplet distribution and was stable to aggregation and creaming over a wide range of pH (5-8), salt concentrations ≤ 100 mM, and heat treatment ≤ 70 °C. Besides, the AFPH-E possessed and maintained strong biological activities, including antihypertensive, antidiabetic, and antioxidant, under different food processing conditions (pH 5-8; NaCl: 50-150 mM, and heat treatment: 30-100 °C). Following in vitro gastrointestinal digestion, the antihypertensive and antioxidant activities were unchanged, while a notable increase of 8% was observed for DPPH. However, the antidiabetic activities were partially reduced in the range of 5-11%. Notably, AFPH-E modulated the gut microbiota and short-chain fatty acids (SCFAs), promoting the growth of beneficial bacteria, particularly Bifidobacteria and Lactobacilli, along with increased SCFA acetate, propionate, and butyrate. Also, AFPH-E up to 10 mg mL−1 did not affect the proliferation of the normal colon cells. In the current work, AFPH demonstrated dual functionality as a plant-based emulsifier with strong biological activities in an oil/water emulsion system and promoted healthy changes in the human gut microbiota. Royal Society of Chemistry 2024-05-27 Article PeerReviewed Qoms, Mohammed S. and Arulrajah, Brisha and Wan Ibadullah, Wan Zunairah and Ramli, Nurul Shazini and Chau, De-Ming and Sarbini, Shahrul Razid and Saari, Nazamid (2024) Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells. Food and Function, 15 (12). pp. 6578-6596. ISSN 2042-6496; eISSN: 2042-650X https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo00377b 10.1039/d4fo00377b |
| spellingShingle | Qoms, Mohammed S. Arulrajah, Brisha Wan Ibadullah, Wan Zunairah Ramli, Nurul Shazini Chau, De-Ming Sarbini, Shahrul Razid Saari, Nazamid Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title | Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title_full | Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title_fullStr | Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title_full_unstemmed | Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title_short | Performance of Azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an O/W emulsion system and their effect on human gut microbiota and mammalian cells |
| title_sort | performance of azolla pinnata fern protein hydrolysates as an emulsifier and nutraceutical ingredient in an o/w emulsion system and their effect on human gut microbiota and mammalian cells |
| url | http://psasir.upm.edu.my/id/eprint/116073/ http://psasir.upm.edu.my/id/eprint/116073/ http://psasir.upm.edu.my/id/eprint/116073/ |